ID   HES2_RAT                Reviewed;         157 AA.
AC   P35429;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Transcription factor HES-2;
DE   AltName: Full=Hairy and enhancer of split 2;
GN   Name=Hes2; Synonyms=Hes-2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Embryonic brain;
RX   PubMed=8354270; DOI=10.1111/j.1432-1033.1993.tb18075.x;
RA   Ishibashi M., Sasai Y., Nakanishi S., Kageyama R.;
RT   "Molecular characterization of HES-2, a mammalian helix-loop-helix factor
RT   structurally related to Drosophila hairy and Enhancer of split.";
RL   Eur. J. Biochem. 215:645-652(1993).
CC   -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC       protein for their transcription.
CC   -!- SUBUNIT: Transcription repression requires formation of a complex with
CC       a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC       interrupting proline) that binds to the N-box (CACNAG), rather than the
CC       canonical E-box (CANNTG).
CC   -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC       domain necessary for the interaction with Groucho/TLE family members,
CC       transcriptional corepressors recruited to specific target DNA by Hairy-
CC       related proteins. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D14029; BAA03118.1; -; mRNA.
DR   PIR; S35037; S35037.
DR   RefSeq; NP_062109.1; NM_019236.1.
DR   AlphaFoldDB; P35429; -.
DR   SMR; P35429; -.
DR   ELM; P35429; -.
DR   STRING; 10116.ENSRNOP00000014035; -.
DR   PaxDb; P35429; -.
DR   Ensembl; ENSRNOT00000014035; ENSRNOP00000014035; ENSRNOG00000010490.
DR   GeneID; 29567; -.
DR   KEGG; rno:29567; -.
DR   UCSC; RGD:62082; rat.
DR   CTD; 54626; -.
DR   RGD; 62082; Hes2.
DR   eggNOG; KOG4304; Eukaryota.
DR   GeneTree; ENSGT00940000161602; -.
DR   HOGENOM; CLU_068550_5_0_1; -.
DR   InParanoid; P35429; -.
DR   OMA; THCANEV; -.
DR   OrthoDB; 1427802at2759; -.
DR   PhylomeDB; P35429; -.
DR   TreeFam; TF351373; -.
DR   PRO; PR:P35429; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Bgee; ENSRNOG00000010490; Expressed in heart and 12 other tissues.
DR   Genevisible; P35429; RN.
DR   GO; GO:0005634; C:nucleus; TAS:RGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003690; F:double-stranded DNA binding; IDA:RGD.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:NTNU_SB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:RGD.
DR   GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:RGD.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR003650; Orange_dom.
DR   Pfam; PF07527; Hairy_orange; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SMART; SM00511; ORANGE; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   PROSITE; PS51054; ORANGE; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..157
FT                   /note="Transcription factor HES-2"
FT                   /id="PRO_0000127208"
FT   DOMAIN          13..70
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   DOMAIN          86..119
FT                   /note="Orange"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT   REGION          124..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           154..157
FT                   /note="WRPW motif"
FT   COMPBIAS        134..157
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   157 AA;  17028 MW;  B5D621E814AE0369 CRC64;
     MRLPRGVGDA AELRKSLKPL LEKRRRARIN ESLSQLKGLV LPLLGAETSR YSKLEKADIL
     EMTVRFLREQ PASVCSTEAP GSLDSYLEGY RACLARLARV LPACSVLEPA VSARLLEHLR
     QRTVSGGPPS LTPASASAPA PSPPVPPPSS LGLWRPW