HES3_HUMAN
ID HES3_HUMAN Reviewed; 186 AA.
AC Q5TGS1; Q5TGS0;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Transcription factor HES-3;
DE AltName: Full=Class B basic helix-loop-helix protein 43;
DE Short=bHLHb43;
DE AltName: Full=Hairy and enhancer of split 3;
GN Name=HES3; Synonyms=BHLHB43;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [2]
RP IDENTIFICATION.
RX PubMed=15254753;
RA Katoh M., Katoh M.;
RT "Identification and characterization of human HES2, HES3, and HES5 genes in
RT silico.";
RL Int. J. Oncol. 25:529-534(2004).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription. {ECO:0000250}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; AL031847; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS41238.1; -.
DR RefSeq; NP_001019769.1; NM_001024598.3.
DR AlphaFoldDB; Q5TGS1; -.
DR SMR; Q5TGS1; -.
DR BioGRID; 133754; 6.
DR STRING; 9606.ENSP00000367130; -.
DR BioMuta; HES3; -.
DR DMDM; 74746514; -.
DR MassIVE; Q5TGS1; -.
DR PaxDb; Q5TGS1; -.
DR PRIDE; Q5TGS1; -.
DR Antibodypedia; 62181; 53 antibodies from 14 providers.
DR DNASU; 390992; -.
DR Ensembl; ENST00000377898.4; ENSP00000367130.3; ENSG00000173673.8.
DR GeneID; 390992; -.
DR KEGG; hsa:390992; -.
DR MANE-Select; ENST00000377898.4; ENSP00000367130.3; NM_001024598.4; NP_001019769.1.
DR UCSC; uc009vly.2; human.
DR CTD; 390992; -.
DR DisGeNET; 390992; -.
DR GeneCards; HES3; -.
DR HGNC; HGNC:26226; HES3.
DR HPA; ENSG00000173673; Not detected.
DR MIM; 609971; gene.
DR neXtProt; NX_Q5TGS1; -.
DR OpenTargets; ENSG00000173673; -.
DR PharmGKB; PA142671692; -.
DR VEuPathDB; HostDB:ENSG00000173673; -.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00730000111482; -.
DR HOGENOM; CLU_068550_4_0_1; -.
DR InParanoid; Q5TGS1; -.
DR OMA; VWRPWSP; -.
DR OrthoDB; 1095795at2759; -.
DR PhylomeDB; Q5TGS1; -.
DR TreeFam; TF351373; -.
DR PathwayCommons; Q5TGS1; -.
DR BioGRID-ORCS; 390992; 13 hits in 1089 CRISPR screens.
DR GenomeRNAi; 390992; -.
DR Pharos; Q5TGS1; Tbio.
DR PRO; PR:Q5TGS1; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q5TGS1; protein.
DR Bgee; ENSG00000173673; Expressed in olfactory segment of nasal mucosa.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR GO; GO:0071820; F:N-box binding; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 3: Inferred from homology;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..186
FT /note="Transcription factor HES-3"
FT /id="PRO_0000269174"
FT DOMAIN 1..49
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 65..99
FT /note="Orange"
FT REGION 128..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 175..178
FT /note="WRPW motif"
FT COMPBIAS 131..155
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 186 AA; 19968 MW; F64A0949CBB0654D CRC64;
MEKKRRARIN VSLEQLKSLL EKHYSHQIRK RKLEKADILE LSVKYMRSLQ NSLQGLWPVP
RGAEQPSGFR SCLPGVSQLL RRGDEVGSGL RCPLVPESAA GSTMDSAGLG QEAPALFRPC
TPAVWAPAPA AGGPRSPPPL LLLPESLPGS SASVPPPQPA SSRCAESPGL GLRVWRPWGS
PGDDLN
