HES3_MOUSE
ID HES3_MOUSE Reviewed; 175 AA.
AC Q61657; O09083; O09150; Q8BTI2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Transcription factor HES-3;
DE AltName: Full=Hairy and enhancer of split 3;
GN Name=Hes3; Synonyms=Hes-3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=8074710; DOI=10.1006/bbrc.1994.2224;
RA Sakagami T., Sakurada K., Sakai Y., Watanabe T., Nakanishi S., Kageyama R.;
RT "Structure and chromosomal locus of the mouse gene encoding a cerebellar
RT Purkinje cell-specific helix-loop-helix factor Hes-3.";
RL Biochem. Biophys. Res. Commun. 203:594-601(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RA Kageyama R.;
RL Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; D32200; BAA06899.1; -; Genomic_DNA.
DR EMBL; D85169; BAA19799.1; -; Genomic_DNA.
DR EMBL; AK090218; BAC41140.1; -; mRNA.
DR EMBL; BC116444; AAI16445.1; -; mRNA.
DR CCDS; CCDS18997.1; -.
DR RefSeq; NP_032263.2; NM_008237.4.
DR RefSeq; XP_011248495.1; XM_011250193.2.
DR AlphaFoldDB; Q61657; -.
DR SMR; Q61657; -.
DR ELM; Q61657; -.
DR STRING; 10090.ENSMUSP00000092006; -.
DR PaxDb; Q61657; -.
DR PRIDE; Q61657; -.
DR Antibodypedia; 62181; 53 antibodies from 14 providers.
DR DNASU; 15207; -.
DR Ensembl; ENSMUST00000094438; ENSMUSP00000092006; ENSMUSG00000028946.
DR GeneID; 15207; -.
DR KEGG; mmu:15207; -.
DR UCSC; uc008wac.1; mouse.
DR CTD; 390992; -.
DR MGI; MGI:104877; Hes3.
DR VEuPathDB; HostDB:ENSMUSG00000028946; -.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00730000111482; -.
DR HOGENOM; CLU_068550_4_0_1; -.
DR InParanoid; Q61657; -.
DR OrthoDB; 1095795at2759; -.
DR PhylomeDB; Q61657; -.
DR TreeFam; TF351373; -.
DR BioGRID-ORCS; 15207; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q61657; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q61657; protein.
DR Bgee; ENSMUSG00000028946; Expressed in spinal cord neural plate and 100 other tissues.
DR ExpressionAtlas; Q61657; baseline and differential.
DR Genevisible; Q61657; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR GO; GO:0071820; F:N-box binding; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:NTNU_SB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0021575; P:hindbrain morphogenesis; IGI:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IGI:MGI.
DR GO; GO:0030901; P:midbrain development; IGI:MGI.
DR GO; GO:0021555; P:midbrain-hindbrain boundary morphogenesis; IGI:MGI.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0021915; P:neural tube development; IGI:MGI.
DR GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR GO; GO:0021557; P:oculomotor nerve development; IGI:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0050767; P:regulation of neurogenesis; IGI:MGI.
DR GO; GO:0060164; P:regulation of timing of neuron differentiation; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0021558; P:trochlear nerve development; IGI:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..175
FT /note="Transcription factor HES-3"
FT /id="PRO_0000127209"
FT DOMAIN 1..49
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 65..98
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 126..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 172..175
FT /note="WRPW motif"
FT CONFLICT 70
FT /note="Q -> H (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="H -> Q (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 175 AA; 19294 MW; CB5FEAD4A64A7F62 CRC64;
MEKKRRARIN VSLEQLRSLL ERHYSHQIRK RKLEKADILE LSVKYMRSLQ NSLQGLWPVP
SGVDYPSGFQ GGLRGVSQRL RPGEGDSGLR CPLLLQRREG STTDSANPQA TSVLNPCLPA
IWAPSRAAGG SHSPQSPLPL PGGLLESSTD VVAPHPASNC QAESTRPGFR VWRPW
