HES3_RAT
ID HES3_RAT Reviewed; 175 AA.
AC Q04667;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Transcription factor HES-3;
DE AltName: Full=Hairy and enhancer of split 3;
GN Name=Hes3; Synonyms=Hes-3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=1340473; DOI=10.1101/gad.6.12b.2620;
RA Sasai Y., Kageyama R., Tagawa Y., Shigemoto R., Nakanishi S.;
RT "Two mammalian helix-loop-helix factors structurally related to Drosophila
RT hairy and Enhancer of split.";
RL Genes Dev. 6:2620-2634(1992).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in Purkinje cells.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; D13418; BAA02683.1; -; mRNA.
DR PIR; S36749; S36749.
DR RefSeq; NP_073178.1; NM_022687.1.
DR RefSeq; XP_008762603.1; XM_008764381.2.
DR RefSeq; XP_008762604.1; XM_008764382.2.
DR RefSeq; XP_008762605.1; XM_008764383.1.
DR RefSeq; XP_017449101.1; XM_017593612.1.
DR RefSeq; XP_017449102.1; XM_017593613.1.
DR RefSeq; XP_017449103.1; XM_017593614.1.
DR AlphaFoldDB; Q04667; -.
DR SMR; Q04667; -.
DR ELM; Q04667; -.
DR STRING; 10116.ENSRNOP00000014535; -.
DR PaxDb; Q04667; -.
DR Ensembl; ENSRNOT00000014536; ENSRNOP00000014535; ENSRNOG00000010893.
DR GeneID; 64628; -.
DR KEGG; rno:64628; -.
DR CTD; 390992; -.
DR RGD; 621339; Hes3.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00730000111482; -.
DR HOGENOM; CLU_068550_4_0_1; -.
DR InParanoid; Q04667; -.
DR OMA; VWRPWSP; -.
DR OrthoDB; 1095795at2759; -.
DR PhylomeDB; Q04667; -.
DR TreeFam; TF351373; -.
DR PRO; PR:Q04667; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000010893; Expressed in cerebellum.
DR Genevisible; Q04667; RN.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR GO; GO:0071820; F:N-box binding; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0021575; P:hindbrain morphogenesis; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0030901; P:midbrain development; ISO:RGD.
DR GO; GO:0021555; P:midbrain-hindbrain boundary morphogenesis; ISO:RGD.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:RGD.
DR GO; GO:0021915; P:neural tube development; ISO:RGD.
DR GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR GO; GO:0021557; P:oculomotor nerve development; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0050767; P:regulation of neurogenesis; ISO:RGD.
DR GO; GO:0060164; P:regulation of timing of neuron differentiation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0021558; P:trochlear nerve development; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..175
FT /note="Transcription factor HES-3"
FT /id="PRO_0000127210"
FT DOMAIN 1..49
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 65..98
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 124..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 172..175
FT /note="WRPW motif"
SQ SEQUENCE 175 AA; 19137 MW; D490663869155CB3 CRC64;
MEKKRRARIN LSLEQLRSLL ERHYSHQIRK RKLEKADILE LSVKYVRSLQ NSLQGLWLVP
SGVDYPSGFR GGLPGSSQRL RPGEDDSGLR CPLLLQRRAG STTDSANPQT ASVLSPCLPA
IWAPGPPAGG SQSPQSPFPP LGGLLESSTG ILAPPPASNC QAENPRPGFR VWRPW
