HES5_MOUSE
ID HES5_MOUSE Reviewed; 167 AA.
AC P70120; Q8BVI1;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Transcription factor HES-5;
DE AltName: Full=Hairy and enhancer of split 5;
GN Name=Hes5; Synonyms=Hes-5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv; TISSUE=Liver;
RX PubMed=7836401; DOI=10.1074/jbc.270.3.1342;
RA Takebayashi K., Akazawa C., Nakanishi S., Kageyama R.;
RT "Structure and promoter analysis of the gene encoding the mouse helix-loop-
RT helix factor HES-5. Identification of the neural precursor cell-specific
RT promoter element.";
RL J. Biol. Chem. 270:1342-1349(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye, and Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION AS NEUROGENESIS REPRESSOR.
RX PubMed=23160044; DOI=10.1038/nn.3264;
RA Tiberi L., van den Ameele J., Dimidschstein J., Piccirilli J., Gall D.,
RA Herpoel A., Bilheu A., Bonnefont J., Iacovino M., Kyba M., Bouschet T.,
RA Vanderhaeghen P.;
RT "BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression
RT of selective Notch targets.";
RL Nat. Neurosci. 15:1627-1635(2012).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription. Plays an important role as
CC neurogenesis negative regulator. {ECO:0000269|PubMed:23160044}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; D32132; BAA06858.1; -; Genomic_DNA.
DR EMBL; AK078170; BAC37159.1; -; mRNA.
DR EMBL; AK087508; BAC39904.1; -; mRNA.
DR CCDS; CCDS19018.1; -.
DR PIR; A55438; A55438.
DR RefSeq; NP_034549.1; NM_010419.4.
DR AlphaFoldDB; P70120; -.
DR SMR; P70120; -.
DR BioGRID; 200278; 2.
DR ELM; P70120; -.
DR STRING; 10090.ENSMUSP00000051118; -.
DR iPTMnet; P70120; -.
DR PhosphoSitePlus; P70120; -.
DR PaxDb; P70120; -.
DR PeptideAtlas; P70120; -.
DR PRIDE; P70120; -.
DR Antibodypedia; 26785; 223 antibodies from 35 providers.
DR DNASU; 15208; -.
DR Ensembl; ENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
DR GeneID; 15208; -.
DR KEGG; mmu:15208; -.
DR UCSC; uc008wck.1; mouse.
DR CTD; 388585; -.
DR MGI; MGI:104876; Hes5.
DR VEuPathDB; HostDB:ENSMUSG00000048001; -.
DR eggNOG; ENOG502S6S1; Eukaryota.
DR GeneTree; ENSGT00940000162836; -.
DR HOGENOM; CLU_068550_3_1_1; -.
DR InParanoid; P70120; -.
DR OMA; LSHCEVQ; -.
DR OrthoDB; 1378299at2759; -.
DR PhylomeDB; P70120; -.
DR TreeFam; TF351373; -.
DR BioGRID-ORCS; 15208; 1 hit in 74 CRISPR screens.
DR PRO; PR:P70120; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P70120; protein.
DR Bgee; ENSMUSG00000048001; Expressed in medial ganglionic eminence and 158 other tissues.
DR ExpressionAtlas; P70120; baseline and differential.
DR Genevisible; P70120; MM.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0003682; F:chromatin binding; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0003690; F:double-stranded DNA binding; ISO:MGI.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0048708; P:astrocyte differentiation; IDA:UniProtKB.
DR GO; GO:0030509; P:BMP signaling pathway; IGI:MGI.
DR GO; GO:0007420; P:brain development; IMP:UniProtKB.
DR GO; GO:0043010; P:camera-type eye development; IMP:UniProtKB.
DR GO; GO:0051216; P:cartilage development; IMP:UniProtKB.
DR GO; GO:0007155; P:cell adhesion; IGI:MGI.
DR GO; GO:0048469; P:cell maturation; IGI:MGI.
DR GO; GO:0022010; P:central nervous system myelination; IMP:MGI.
DR GO; GO:0072049; P:comma-shaped body morphogenesis; IEP:UniProtKB.
DR GO; GO:0090162; P:establishment of epithelial cell polarity; IGI:MGI.
DR GO; GO:0021861; P:forebrain radial glial cell differentiation; ISO:MGI.
DR GO; GO:0021781; P:glial cell fate commitment; IMP:MGI.
DR GO; GO:0042491; P:inner ear auditory receptor cell differentiation; IGI:MGI.
DR GO; GO:0060122; P:inner ear receptor cell stereocilium organization; IGI:MGI.
DR GO; GO:0072282; P:metanephric nephron tubule morphogenesis; IEP:UniProtKB.
DR GO; GO:0048712; P:negative regulation of astrocyte differentiation; IDA:UniProtKB.
DR GO; GO:2000978; P:negative regulation of forebrain neuron differentiation; ISO:MGI.
DR GO; GO:0045608; P:negative regulation of inner ear auditory receptor cell differentiation; IMP:MGI.
DR GO; GO:2000981; P:negative regulation of inner ear receptor cell differentiation; IMP:UniProtKB.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IMP:MGI.
DR GO; GO:0048715; P:negative regulation of oligodendrocyte differentiation; IDA:UniProtKB.
DR GO; GO:2000974; P:negative regulation of pro-B cell differentiation; ISS:UniProtKB.
DR GO; GO:2000737; P:negative regulation of stem cell differentiation; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0021915; P:neural tube development; IGI:MGI.
DR GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR GO; GO:0097150; P:neuronal stem cell population maintenance; IMP:UniProtKB.
DR GO; GO:0007219; P:Notch signaling pathway; IDA:MGI.
DR GO; GO:0014003; P:oligodendrocyte development; IMP:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:MGI.
DR GO; GO:0030513; P:positive regulation of BMP signaling pathway; IGI:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:UniProtKB.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IDA:UniProtKB.
DR GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IDA:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; IDA:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:MGI.
DR GO; GO:0050678; P:regulation of epithelial cell proliferation; IGI:MGI.
DR GO; GO:0031641; P:regulation of myelination; IMP:MGI.
DR GO; GO:0050767; P:regulation of neurogenesis; IGI:MGI.
DR GO; GO:0045664; P:regulation of neuron differentiation; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0072050; P:S-shaped body morphogenesis; IEP:UniProtKB.
DR GO; GO:0007224; P:smoothened signaling pathway; IDA:MGI.
DR GO; GO:0072086; P:specification of loop of Henle identity; IEP:UniProtKB.
DR GO; GO:0021537; P:telencephalon development; IGI:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..167
FT /note="Transcription factor HES-5"
FT /id="PRO_0000127212"
FT DOMAIN 16..72
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 88..119
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 124..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 164..167
FT /note="WRPW motif"
FT COMPBIAS 125..139
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 22
FT /note="P -> S (in Ref. 2; BAC37159)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="W -> L (in Ref. 2; BAC37159)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 167 AA; 18425 MW; CA0E697094F4E760 CRC64;
MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH QPNSKLEKAD
ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV QFLTLHAASD TQMKLLYHFQ
RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA AAVSTSRQPA CGLWRPW
