HESX1_MOUSE
ID HESX1_MOUSE Reviewed; 185 AA.
AC Q61658; Q61047; Q8C477;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Homeobox expressed in ES cells 1;
DE AltName: Full=Anterior-restricted homeobox protein;
DE AltName: Full=Homeobox protein ANF;
DE AltName: Full=Rathke pouch homeo box;
GN Name=Hesx1; Synonyms=Hes-1, Rpx;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/Sv; TISSUE=Embryonic stem cell;
RX PubMed=7876132; DOI=10.1074/jbc.270.8.3869;
RA Thomas P.Q., Johnson B.V., Rathjen J., Rathjen P.D.;
RT "Sequence, genomic organization, and expression of the novel homeobox gene
RT Hesx1.";
RL J. Biol. Chem. 270:3869-3875(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=8565852; DOI=10.1242/dev.122.1.41;
RA Hermesz E., Mackem S., Mahon K.A.;
RT "Rpx: a novel anterior-restricted homeobox gene progressively activated in
RT the prechordal plate, anterior neural plate and Rathke's pouch of the mouse
RT embryo.";
RL Development 122:41-52(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=1360650; DOI=10.1093/nar/20.21.5840;
RA Thomas P.Q., Rathjen P.D.;
RT "HES-1, a novel homeobox gene expressed by murine embryonic stem cells,
RT identifies a new class of homeobox genes.";
RL Nucleic Acids Res. 20:5840-5840(1992).
RN [7]
RP FUNCTION.
RX PubMed=9620767; DOI=10.1038/477;
RA Dattani M.T., Martinez-Barbera J.-P., Thomas P.Q., Brickman J.M., Gupta R.,
RA Maartensson I.-L., Toresson H., Fox M., Wales J.K.H., Hindmarsh P.C.,
RA Krauss S., Beddington R.S.P., Robinson I.C.A.F.;
RT "Mutations in the homeobox gene HESX1/Hesx1 associated with septo-optic
RT dysplasia in human and mouse.";
RL Nat. Genet. 19:125-133(1998).
CC -!- FUNCTION: Required for the normal development of the forebrain, eyes
CC and other anterior structures such as the olfactory placodes and
CC pituitary gland. Possible transcriptional repressor. Binds to the
CC palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1
CC and PROP1 bind as heterodimers on this palindromic site, and, in vitro,
CC HESX1 can antagonize PROP1 activation. {ECO:0000269|PubMed:9620767}.
CC -!- SUBUNIT: Can form heterodimers with PROP1 in binding to DNA Interacts
CC with TLE1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: High levels found in the embryonic liver, lower
CC level expression seen in the viscera, amnion and yolk sac.
CC -!- DEVELOPMENTAL STAGE: Early expression seen in the anterior midline
CC endoderm and prechordal plate precursor, subsequently activated in the
CC overlying ectoderm of the cephalic neural plate. Later disappears in
CC the mesendoderm while remaining in the prospective prosencephalic
CC region of the neural plate ectoderm, ultimately expression is
CC restricted to Rathke pouch, the primordium of the pituitary. Expressed
CC in embryonic stem cells. {ECO:0000269|PubMed:1360650}.
CC -!- INDUCTION: Down-regulated during embryonic stem (ES) cell
CC differentiation.
CC -!- SIMILARITY: Belongs to the ANF homeobox family. {ECO:0000305}.
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DR EMBL; X80040; CAA56344.1; -; mRNA.
DR EMBL; U40720; AAA97869.1; -; mRNA.
DR EMBL; AK082831; BAC38643.1; -; mRNA.
DR EMBL; AC124603; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC132051; AAI32052.1; -; mRNA.
DR EMBL; BC132053; AAI32054.1; -; mRNA.
DR EMBL; BC145262; AAI45263.1; -; mRNA.
DR CCDS; CCDS26884.1; -.
DR PIR; A55882; A55882.
DR RefSeq; NP_034550.2; NM_010420.2.
DR RefSeq; XP_006518633.1; XM_006518570.3.
DR AlphaFoldDB; Q61658; -.
DR SMR; Q61658; -.
DR BioGRID; 200279; 5.
DR STRING; 10090.ENSMUSP00000041999; -.
DR PhosphoSitePlus; Q61658; -.
DR PaxDb; Q61658; -.
DR PRIDE; Q61658; -.
DR Antibodypedia; 14999; 103 antibodies from 21 providers.
DR DNASU; 15209; -.
DR Ensembl; ENSMUST00000035433; ENSMUSP00000041999; ENSMUSG00000040726.
DR Ensembl; ENSMUST00000224331; ENSMUSP00000152952; ENSMUSG00000040726.
DR GeneID; 15209; -.
DR KEGG; mmu:15209; -.
DR UCSC; uc007stj.1; mouse.
DR CTD; 8820; -.
DR MGI; MGI:96071; Hesx1.
DR VEuPathDB; HostDB:ENSMUSG00000040726; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000156780; -.
DR HOGENOM; CLU_125528_0_0_1; -.
DR InParanoid; Q61658; -.
DR OMA; MDACTNL; -.
DR OrthoDB; 1270742at2759; -.
DR PhylomeDB; Q61658; -.
DR TreeFam; TF335506; -.
DR BioGRID-ORCS; 15209; 0 hits in 76 CRISPR screens.
DR PRO; PR:Q61658; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q61658; protein.
DR Bgee; ENSMUSG00000040726; Expressed in remnant of Rathke's pouch and 45 other tissues.
DR Genevisible; Q61658; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; ISO:MGI.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:MGI.
DR GO; GO:0047485; F:protein N-terminus binding; IPI:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IMP:MGI.
DR GO; GO:0007420; P:brain development; IMP:MGI.
DR GO; GO:0043010; P:camera-type eye development; IMP:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IMP:MGI.
DR GO; GO:0071276; P:cellular response to cadmium ion; IDA:MGI.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; IMP:MGI.
DR GO; GO:0030900; P:forebrain development; IDA:MGI.
DR GO; GO:0048853; P:forebrain morphogenesis; IMP:MGI.
DR GO; GO:0010467; P:gene expression; IDA:MGI.
DR GO; GO:0008406; P:gonad development; IGI:MGI.
DR GO; GO:0048861; P:leukemia inhibitory factor signaling pathway; IMP:MGI.
DR GO; GO:0035264; P:multicellular organism growth; IGI:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0043584; P:nose development; IMP:MGI.
DR GO; GO:0030916; P:otic vesicle formation; IMP:MGI.
DR GO; GO:0021983; P:pituitary gland development; IMP:MGI.
DR GO; GO:0045995; P:regulation of embryonic development; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048863; P:stem cell differentiation; IMP:MGI.
DR GO; GO:0019827; P:stem cell population maintenance; IMP:MGI.
DR GO; GO:0030878; P:thyroid gland development; IGI:MGI.
DR GO; GO:0016055; P:Wnt signaling pathway; IDA:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR043402; Hesx1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR PANTHER; PTHR46966; PTHR46966; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..185
FT /note="Homeobox expressed in ES cells 1"
FT /id="PRO_0000048923"
FT DNA_BIND 108..167
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 32..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 51
FT /note="D -> N (in Ref. 1; CAA56344 and 2; AAA97869)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="G -> D (in Ref. 1; CAA56344 and 2; AAA97869)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="K -> R (in Ref. 1; CAA56344)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 185 AA; 21504 MW; 5C18A524BF281246 CRC64;
MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG DSEKGGNPPL
HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK RELSWYRGRR PRTAFTQNQV
EVLENVFRVN CYPGIDIRED LAQKLNLEED RIQIWFQNRR AKMKRSRRES QFLMAKKPFN
PDLLK
