ID   HESX1_XENTR             Reviewed;         186 AA.
AC   Q28J15;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Homeobox expressed in ES cells 1;
DE   AltName: Full=Homeobox protein ANF-1;
GN   Name=hesx1 {ECO:0000312|EMBL:CAJ82725.1}; ORFNames=TNeu069m07.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1] {ECO:0000312|EMBL:CAJ82725.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula {ECO:0000312|EMBL:CAJ82725.1};
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:CAJ82725.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula {ECO:0000312|EMBL:AAI59021.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulates the earliest stages of development of the anterior
CC       neural plate. Plays a role in forebrain development by inhibiting the
CC       expression of otx2 and pax6 in the rostral region of the anterior
CC       neural plate. Necessary for both neural differentiation and neural
CC       patterning. Controls Spemann organizer development. May act as a
CC       transcriptional repressor (By similarity).
CC       {ECO:0000250|UniProtKB:Q91898}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with zyx. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q91898,
CC       ECO:0000255|PROSITE-ProRule:PRU00108}. Note=Enters the cytoplasm in the
CC       presence of zyx. {ECO:0000250|UniProtKB:Q91898}.
CC   -!- SIMILARITY: Belongs to the ANF homeobox family. {ECO:0000255}.
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DR   EMBL; CR760108; CAJ82725.1; -; mRNA.
DR   EMBL; BC159020; AAI59021.1; -; mRNA.
DR   RefSeq; NP_001016712.1; NM_001016712.2.
DR   AlphaFoldDB; Q28J15; -.
DR   SMR; Q28J15; -.
DR   STRING; 8364.ENSXETP00000019070; -.
DR   PaxDb; Q28J15; -.
DR   GeneID; 549466; -.
DR   KEGG; xtr:549466; -.
DR   CTD; 8820; -.
DR   Xenbase; XB-GENE-483193; hesx1.
DR   eggNOG; KOG0490; Eukaryota.
DR   HOGENOM; CLU_125528_0_0_1; -.
DR   InParanoid; Q28J15; -.
DR   OrthoDB; 1270742at2759; -.
DR   PhylomeDB; Q28J15; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000008710; Expressed in immature eye and 8 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030900; P:forebrain development; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0021983; P:pituitary gland development; IBA:GO_Central.
DR   GO; GO:0045664; P:regulation of neuron differentiation; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0060061; P:Spemann organizer formation; ISS:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR043402; Hesx1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   PANTHER; PTHR46966; PTHR46966; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..186
FT                   /note="Homeobox expressed in ES cells 1"
FT                   /id="PRO_0000331486"
FT   DNA_BIND        109..168
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
SQ   SEQUENCE   186 AA;  21688 MW;  C3E38E7EAAF3CCBB CRC64;
     MSPGLQKGSR LMENRSPPSS FSIEHILGLD KKTEVASSPI IKHHRPWIQC NSEGVDGTFW
     HIPVISCDLP VQVHALRRSM GEETQVRLEK CCGEEDRLTY KRELSWYRGR RPRTAFTRSQ
     IEILENVFRV NSYPGIDIRE ELAGKLALDE DRIQIWFQNR RAKLKRSHRE SQFLMVKDSL
     SSKIQE