ID   HESXA_XENLA             Reviewed;         182 AA.
AC   Q91617; B7ZQL4;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Homeobox expressed in ES cells 1-A;
DE   AltName: Full=Homeobox protein ANF-2;
DE            Short=xANF-2;
GN   Name=hesx1-a; Synonyms=anf2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND INDUCTION.
RC   TISSUE=Pituitary anterior lobe;
RX   PubMed=7556943; DOI=10.1006/dbio.1995.1311;
RA   Mathers P.H., Miller A., Doniach T., Dirksen M.-L., Jamrich M.;
RT   "Initiation of anterior head-specific gene expression in uncommitted
RT   ectoderm of Xenopus laevis by ammonium chloride.";
RL   Dev. Biol. 171:641-654(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Appears to be involved in the regional specification of the
CC       anterior head of Xenopus embryos. {ECO:0000269|PubMed:7556943}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Initially expressed in the anterior dorsal region
CC       of early embryos and later exclusively in the primordium of the
CC       anterior pituitary gland. {ECO:0000269|PubMed:7556943}.
CC   -!- DEVELOPMENTAL STAGE: Activated during the early gastrula stage of
CC       embryogenesis. Expression is weak between stages 10 and 11 then
CC       increases sharply between stages 11 and 12, peaking at stage 13 and
CC       then sharply declining. Undetectable after stage 35.
CC       {ECO:0000269|PubMed:7556943}.
CC   -!- INDUCTION: By Ammonium chloride. {ECO:0000269|PubMed:7556943}.
CC   -!- SIMILARITY: Belongs to the ANF homeobox family. {ECO:0000305}.
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DR   EMBL; U28370; AAA85270.1; -; mRNA.
DR   EMBL; BC169850; AAI69850.1; -; mRNA.
DR   RefSeq; NP_001079264.1; NM_001085795.1.
DR   AlphaFoldDB; Q91617; -.
DR   SMR; Q91617; -.
DR   GeneID; 378542; -.
DR   KEGG; xla:378542; -.
DR   CTD; 378542; -.
DR   Xenbase; XB-GENE-6252654; hesx1.S.
DR   OrthoDB; 1270742at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 378542; Expressed in neurula embryo and 1 other tissue.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0030900; P:forebrain development; IEA:InterPro.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR043402; Hesx1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   PANTHER; PTHR46966; PTHR46966; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..182
FT                   /note="Homeobox expressed in ES cells 1-A"
FT                   /id="PRO_0000048927"
FT   DNA_BIND        103..163
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   CONFLICT        59
FT                   /note="R -> W (in Ref. 1; AAA85270)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        64
FT                   /note="I -> IS (in Ref. 1; AAA85270)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        92
FT                   /note="E -> D (in Ref. 1; AAA85270)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   182 AA;  21399 MW;  5D3AA16C628DA18D CRC64;
     MSTGLQKGSR LMENRSPPSS FSIEHILGLD KKMDVASSPI IKHHRPWIEC SSKVDGTFRQ
     IPLIYDLPVQ VDAMRRSAEE ETKIRLDRGE EERLTYKREQ SWYRGRRPRT AFTRGQIEIL
     ENVFRVNSYP GIDVREELAS KLALDEDRIQ IWFQNRRAKL KRSHRESQFL IVKDSLSSKI
     EE