HESXB_XENLA
ID HESXB_XENLA Reviewed; 187 AA.
AC Q91898; Q91899;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Homeobox expressed in ES cells 1-B;
DE AltName: Full=Homeobox protein ANF-1;
DE Short=XANF-1;
DE Short=Xanf1;
GN Name=hesx1-b; Synonyms=anf-1, anf1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Ectoderm, and Gastrula;
RX PubMed=1353734; DOI=10.1016/0012-1606(92)90144-6;
RA Zaraisky A.G., Lukyanov S.A., Vasiliev O.L., Smirnov Y.V., Belyavsky A.V.,
RA Kazanskaya O.V.;
RT "A novel homeobox gene expressed in the anterior neural plate of the
RT Xenopus embryo.";
RL Dev. Biol. 152:373-382(1992).
RN [2]
RP SEQUENCE REVISION.
RA Zaraisky A.G.;
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7909469;
RA Samokhvalov I.M., Semenova N.A., Nikolaev A.I., Beliavskii A.V.;
RT "Genomic structure of the homeo box-containing gene XANF1.";
RL Dokl. Akad. Nauk 334:522-525(1994).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=8582293; DOI=10.1242/dev.121.11.3839;
RA Zaraisky A.G., Ecochard V., Kazanskaya O.V., Lukyanov S.A., Fesenko I.V.,
RA Duprat A.-M.;
RT "The homeobox-containing gene XANF-1 may control development of the Spemann
RT organizer.";
RL Development 121:3839-3847(1995).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9373136; DOI=10.1016/s0378-1119(97)00326-0;
RA Kazanskaya O.V., Severtzova E.A., Barth K.A., Ermakova G.V., Lukyanov S.A.,
RA Benyumov A.O., Pannese M., Boncinelli E., Wilson S.W., Zaraisky A.G.;
RT "Anf: a novel class of vertebrate homeobox genes expressed at the anterior
RT end of the main embryonic axis.";
RL Gene 200:25-34(1997).
RN [6]
RP FUNCTION.
RX PubMed=10498686; DOI=10.1242/dev.126.20.4513;
RA Ermakova G.V., Alexandrova E.M., Kazanskaya O.V., Vasiliev O.L.,
RA Smith M.W., Zaraisky A.G.;
RT "The homeobox gene, Xanf-1, can control both neural differentiation and
RT patterning in the presumptive anterior neurectoderm of the Xenopus laevis
RT embryo.";
RL Development 126:4513-4523(1999).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=12039055; DOI=10.1016/s0378-1119(02)00393-1;
RA Eroshkin F., Kazanskaya O., Martynova N., Zaraisky A.;
RT "Characterization of cis-regulatory elements of the homeobox gene Xanf-1.";
RL Gene 285:279-286(2002).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=15128667; DOI=10.1242/dev.01133;
RA Martynova N., Eroshkin F., Ermakova G., Bayramov A., Gray J., Grainger R.,
RA Zaraisky A.;
RT "Patterning the forebrain: FoxA4a/Pintallavis and Xvent2 determine the
RT posterior limit of Xanf1 expression in the neural plate.";
RL Development 131:2329-2338(2004).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=15511636; DOI=10.1016/j.mod.2004.08.002;
RA Bayramov A.V., Martynova N.Y., Eroshkin F.M., Ermakova G.V., Zaraisky A.G.;
RT "The homeodomain-containing transcription factor X-nkx-5.1 inhibits
RT expression of the homeobox gene Xanf-1 during the Xenopus laevis forebrain
RT development.";
RL Mech. Dev. 121:1425-1441(2004).
RN [10]
RP FUNCTION.
RX PubMed=17511981; DOI=10.1016/j.ydbio.2007.03.524;
RA Ermakova G.V., Solovieva E.A., Martynova N.Y., Zaraisky A.G.;
RT "The homeodomain factor Xanf represses expression of genes in the
RT presumptive rostral forebrain that specify more caudal brain regions.";
RL Dev. Biol. 307:483-497(2007).
RN [11]
RP INTERACTION WITH ZYX, AND SUBCELLULAR LOCATION.
RX PubMed=18297730; DOI=10.1002/dvdy.21471;
RA Martynova N.Y., Eroshkin F.M., Ermolina L.V., Ermakova G.V.,
RA Korotaeva A.L., Smurova K.M., Gyoeva F.K., Zaraisky A.G.;
RT "The LIM-domain protein zyxin binds the homeodomain factor Xanf1/Hesx1 and
RT modulates its activity in the anterior neural plate of Xenopus laevis
RT embryo.";
RL Dev. Dyn. 237:736-749(2008).
CC -!- FUNCTION: Regulates the earliest stages of development of the anterior
CC neural plate. Plays a role in forebrain development by inhibiting the
CC expression of otx2 and pax6 in the rostral region of the anterior
CC neural plate. Necessary for both neural differentiation and neural
CC patterning. Controls Spemann organizer development. May act as a
CC transcriptional repressor. {ECO:0000269|PubMed:10498686,
CC ECO:0000269|PubMed:1353734, ECO:0000269|PubMed:17511981,
CC ECO:0000269|PubMed:8582293}.
CC -!- SUBUNIT: The N-terminus interacts with the LIM 2 domain of zyx.
CC {ECO:0000269|PubMed:18297730}.
CC -!- INTERACTION:
CC Q91898; A5H447: zyx; NbExp=5; IntAct=EBI-5651634, EBI-5651620;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:18297730}. Note=Enters the cytoplasm in the presence
CC of zyx.
CC -!- TISSUE SPECIFICITY: First expressed at a low level in the late blastula
CC stage (stage 9) in most cells of the animal half of the embryo.
CC Following this, predominantly expressed in two zones; the dorsal
CC blastopore lip (Spemann organizer) at the beginning of gastrulation,
CC and subsequently in the anterior part of the neural anlage (the region
CC of future forebrain). {ECO:0000269|PubMed:12039055,
CC ECO:0000269|PubMed:1353734, ECO:0000269|PubMed:15128667,
CC ECO:0000269|PubMed:15511636, ECO:0000269|PubMed:8582293,
CC ECO:0000269|PubMed:9373136}.
CC -!- SIMILARITY: Belongs to the ANF homeobox family. {ECO:0000305}.
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DR EMBL; X60099; CAA42695.2; -; mRNA.
DR EMBL; X70282; CAA49768.1; -; Genomic_DNA.
DR PIR; S31859; S31859.
DR RefSeq; NP_001156042.1; NM_001162570.1.
DR AlphaFoldDB; Q91898; -.
DR SMR; Q91898; -.
DR IntAct; Q91898; 4.
DR GeneID; 397950; -.
DR KEGG; xla:397950; -.
DR CTD; 397950; -.
DR Xenbase; XB-GENE-864952; hesx1.L.
DR OrthoDB; 1270742at2759; -.
DR Proteomes; UP000186698; Chromosome 4L.
DR Bgee; 397950; Expressed in neurula embryo and 2 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0030274; F:LIM domain binding; IDA:UniProtKB.
DR GO; GO:0001221; F:transcription coregulator binding; IPI:UniProtKB.
DR GO; GO:0030900; P:forebrain development; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0045664; P:regulation of neuron differentiation; IMP:UniProtKB.
DR GO; GO:0060061; P:Spemann organizer formation; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR043402; Hesx1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR PANTHER; PTHR46966; PTHR46966; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..187
FT /note="Homeobox expressed in ES cells 1-B"
FT /id="PRO_0000048926"
FT DNA_BIND 110..169
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT CONFLICT 57
FT /note="N -> D (in Ref. 1; CAA42695)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="C -> F (in Ref. 1; CAA42695)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 21714 MW; 0E9BF0988E1AEFB5 CRC64;
MSPALQKGSS LMENRSPPSS FSIEHILGLD KKTDVASSPI IKHHRPWIEC SSKGVVNGTC
WQIPVIACDL PIQVHAVHRS EEEETKIRLE KCFGDEDRLT YKRELSWYRG RRPRTAFTRS
QIEILENVFR VNSYPGIDVR EELASKLALD EDRIQIWFQN RRAKLKRSHR ESQFLIVKDS
LSSKIQE
