HETQ1_PODAN
ID HETQ1_PODAN Reviewed; 278 AA.
AC B2AXJ5;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Gasdermin-like protein het-Q1 {ECO:0000305};
DE AltName: Full=Heterokaryon incompatibility protein Q1 {ECO:0000303|PubMed:35135876};
DE Contains:
DE RecName: Full=Gasdermin-like protein het-Q1, N-terminal {ECO:0000305};
GN Name=het-Q1 {ECO:0000303|PubMed:35135876};
GN ORFNames=PODANS_7_10775 {ECO:0000312|EMBL:CAP69119.1};
OS Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS (Pleurage anserina).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC Podospora anserina.
OX NCBI_TaxID=515849;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383;
RX PubMed=18460219; DOI=10.1186/gb-2008-9-5-r77;
RA Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M.,
RA Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V.,
RA Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M.,
RA Contamine V., Arnaise S., Bourdais A., Berteaux-Lecellier V., Gautheret D.,
RA de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B.,
RA El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H.,
RA Debuchy R., Wincker P., Weissenbach J., Silar P.;
RT "The genome sequence of the model ascomycete fungus Podospora anserina.";
RL Genome Biol. 9:R77.1-R77.22(2008).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383;
RX PubMed=24558260; DOI=10.1534/genetics.113.159988;
RA Grognet P., Bidard F., Kuchly C., Tong L.C.H., Coppin E., Benkhali J.A.,
RA Couloux A., Wincker P., Debuchy R., Silar P.;
RT "Maintaining two mating types: Structure of the mating type locus and its
RT role in heterokaryosis in Podospora anserina.";
RL Genetics 197:421-432(2014).
RN [3]
RP FUNCTION, PROTEOLYTIC CLEAVAGE, AND MUTAGENESIS OF GLY-234; LYS-235;
RP VAL-236; LEU-237; PHE-238; GLY-240 AND GLY-241.
RX PubMed=35135876; DOI=10.1073/pnas.2109418119;
RA Clave C., Dyrka W., Turcotte E.A., Granger-Farbos A., Ibarlosa L.,
RA Pinson B., Vance R.E., Saupe S.J., Daskalov A.;
RT "Fungal gasdermin-like proteins are controlled by proteolytic cleavage.";
RL Proc. Natl. Acad. Sci. U.S.A. 119:0-0(2022).
CC -!- FUNCTION: [Gasdermin-like protein het-Q1]: Gasdermin-like protein
CC involved in heterokaryon incompatibility, a process that ensures that
CC during spontaneous vegetative cell fusion, only compatible cells from
CC the same colony survive (non-self-recognition) (PubMed:35135876). In
CC P.anserina, the het-q locus exists as 2 incompatible alleles, het-Q1
CC (this entry) and het-Q2 (AC P0DW09) (PubMed:35135876). This form
CC constitutes the precursor of the pore-forming protein: during the
CC allorecognition process, it is cleaved by het-Q2, releasing the N-
CC terminal moiety (Gasdermin-like protein het-Q1, N-terminal) that binds
CC to membranes and forms pores, triggering cell death (PubMed:35135876).
CC {ECO:0000269|PubMed:35135876}.
CC -!- FUNCTION: [Gasdermin-like protein het-Q1, N-terminal]: Pore-forming
CC protein that causes membrane permeabilization and cell death
CC (PubMed:35135876). Released upon cleavage and maturation by het-Q2 and
CC binds to membrane inner leaflet lipids (PubMed:35135876).
CC Homooligomerizes within the membrane and forms pores of 10-15
CC nanometers (nm) of inner diameter, triggering cell death (By
CC similarity). {ECO:0000250|UniProtKB:P57764,
CC ECO:0000269|PubMed:35135876}.
CC -!- SUBUNIT: [Gasdermin-like protein het-Q1, N-terminal]: Homooligomer;
CC forms a homooligomeric ring-shaped pore complex when inserted in the
CC membrane. {ECO:0000250|UniProtKB:P57764}.
CC -!- SUBCELLULAR LOCATION: [Gasdermin-like protein het-Q1, N-terminal]: Cell
CC membrane {ECO:0000250|UniProtKB:P57764}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P57764}.
CC -!- PTM: [Gasdermin-like protein het-Q1]: The precursor form is cleaved by
CC het-Q2, generating the pore-forming protein (Gasdermin-like protein
CC het-Q1, N-terminal). {ECO:0000269|PubMed:35135876}.
CC -!- SIMILARITY: Belongs to the gasdermin family. {ECO:0000305}.
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DR EMBL; CU633900; CAP69119.1; -; Genomic_DNA.
DR EMBL; FO904942; CDP32598.1; -; Genomic_DNA.
DR RefSeq; XP_001908446.1; XM_001908411.1.
DR EnsemblFungi; CAP69119; CAP69119; PODANS_7_10775.
DR GeneID; 6192626; -.
DR KEGG; pan:PODANSg5481; -.
DR VEuPathDB; FungiDB:PODANS_7_10775; -.
DR eggNOG; ENOG502T8QF; Eukaryota.
DR HOGENOM; CLU_1001576_0_0_1; -.
DR OrthoDB; 1535722at2759; -.
DR Proteomes; UP000001197; Chromosome 7.
DR GO; GO:0022829; F:wide pore channel activity; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Necrosis; Reference proteome; Transmembrane;
KW Transmembrane beta strand.
FT CHAIN 1..278
FT /note="Gasdermin-like protein het-Q1"
FT /id="PRO_0000455983"
FT CHAIN 1..237
FT /note="Gasdermin-like protein het-Q1, N-terminal"
FT /evidence="ECO:0000305"
FT /id="PRO_0000455984"
FT SITE 237..238
FT /note="Cleavage; by het-Q2"
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 234
FT /note="G->A: Does not affect ability to trigger cell death
FT during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 235
FT /note="K->A: Does not affect ability to trigger cell death
FT during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 236
FT /note="V->A: Does not affect ability to trigger cell death
FT during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 237
FT /note="L->A: Abolished cleavage by het-Q2 and ability to
FT trigger cell death during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 238
FT /note="F->A: Abolished cleavage by het-Q2 and ability to
FT trigger cell death during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 240
FT /note="G->A: Does not affect ability to trigger cell death
FT during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
FT MUTAGEN 241
FT /note="G->A: Does not affect ability to trigger cell death
FT during the allorecognition process."
FT /evidence="ECO:0000269|PubMed:35135876"
SQ SEQUENCE 278 AA; 29695 MW; FA3AF64A213952A9 CRC64;
MPTKTSQHAF AGSERWVVPR YSSKPGTLIR LGSVLTDPED LESSLNLDSI PPIPPHLLRD
ATPEVRMSVQ TELSKSDSTL AKAAPALEGI LTLGGGVEAS RSQGVSSSLN ISGTVKATVF
RADKSYMDVL LKDKNVISYA KRGLGKPMFV VVGVATAGRV EMKETRHVTR KAGVSGKVGV
EVIGEGEVGL ERERSDKSCN EVRGEGGLDF AYRVREFGYS RVRGTVKDKG DWTGKVLFAG
GKGPVVEKGG EVVPVFKEFK EGEVKLRATG SFDVAAKA
