HETR_NOSP7
ID HETR_NOSP7 Reviewed; 299 AA.
AC Q9AH77; B2J1N0;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=DNA-binding transcriptional activator HetR {ECO:0000255|HAMAP-Rule:MF_00781};
DE EC=3.4.21.- {ECO:0000255|HAMAP-Rule:MF_00781};
DE AltName: Full=Heterocyst differentiation control protein {ECO:0000255|HAMAP-Rule:MF_00781, ECO:0000303|PubMed:11274126};
GN Name=hetR {ECO:0000255|HAMAP-Rule:MF_00781, ECO:0000303|PubMed:11274126};
GN OrderedLocusNames=Npun_R1722;
OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=63737;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION
RP BY NITROGEN LIMITATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 29133 / PCC 73102;
RX PubMed=11274126; DOI=10.1128/jb.183.8.2654-2661.2001;
RA Wong F.C.Y., Meeks J.C.;
RT "The hetF gene product is essential to heterocyst differentiation and
RT affects hetR function in the cyanobacterium Nostoc punctiforme.";
RL J. Bacteriol. 183:2654-2661(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Controls heterocyst differentiation. Dimerization is required
CC for DNA-binding. Has both a protease and a DNA-binding activity (By
CC similarity). Increased expression leads to more heterocysts than usual
CC (PubMed:11274126). {ECO:0000255|HAMAP-Rule:MF_00781,
CC ECO:0000269|PubMed:11274126}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000255|HAMAP-
CC Rule:MF_00781}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in heterocysts (at protein level).
CC {ECO:0000269|PubMed:11274126}.
CC -!- INDUCTION: By nitrogen deficiency. Transcription increases 3-6 hours
CC after nitrogen reduction, peaks at 12 hours and declines slowly after.
CC Under control of both nctA and hetF. {ECO:0000269|PubMed:11274126}.
CC -!- DISRUPTION PHENOTYPE: Unable to differentiate heterocysts.
CC {ECO:0000269|PubMed:11274126}.
CC -!- SIMILARITY: Belongs to the peptidase S48 family. {ECO:0000255|HAMAP-
CC Rule:MF_00781}.
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DR EMBL; AF318069; AAG53680.1; -; Genomic_DNA.
DR EMBL; CP001037; ACC80391.1; -; Genomic_DNA.
DR RefSeq; WP_012408409.1; NC_010628.1.
DR AlphaFoldDB; Q9AH77; -.
DR SMR; Q9AH77; -.
DR STRING; 63737.Npun_R1722; -.
DR MEROPS; S48.001; -.
DR DNASU; 6251161; -.
DR EnsemblBacteria; ACC80391; ACC80391; Npun_R1722.
DR KEGG; npu:Npun_R1722; -.
DR eggNOG; ENOG502Z96Q; Bacteria.
DR HOGENOM; CLU_926376_0_0_3; -.
DR OMA; HHIEPKR; -.
DR OrthoDB; 590352at2; -.
DR PhylomeDB; Q9AH77; -.
DR Proteomes; UP000001191; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043158; P:heterocyst differentiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1670; -; 1.
DR Gene3D; 1.10.10.1680; -; 1.
DR HAMAP; MF_00781; HetR; 1.
DR InterPro; IPR040949; HetR_C.
DR InterPro; IPR041936; HetR_DNA-bd_N.
DR InterPro; IPR041935; HetR_flap.
DR InterPro; IPR005319; Pept_S48_HetR.
DR Pfam; PF18460; HetR_C; 1.
DR Pfam; PF03574; Peptidase_S48; 1.
PE 1: Evidence at protein level;
KW Activator; Disulfide bond; DNA-binding; Heterocyst; Hydrolase; Protease;
KW Reference proteome; Serine protease; Transcription;
KW Transcription regulation.
FT CHAIN 1..299
FT /note="DNA-binding transcriptional activator HetR"
FT /id="PRO_0000208480"
FT ACT_SITE 152
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00781"
FT DISULFID 48
FT /note="Interchain"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00781"
SQ SEQUENCE 299 AA; 34843 MW; AA6D2C6C9FAE94E6 CRC64;
MSNDIDLIKR LDPSAMDQIM LYLAFSAMRT SGHRHGAFLD AAATAAKCAI YMTYLEQGQN
LRMTGHLHHL EPKRVKIIVE EVRQALTEGK LLKMLGSQEP RYLIQLPYVW LEKYPWQPGR
SRVPGTSLTS EEKRQIEQKL PSNLPDAQLV SSFEFLDLIE FLHRRSQEDL PTEHQMPLSE
ALGEHIKRRL LYSGTVTRID SPWGMPFYAL TRPFYAPADD QERTYIMVED TARYFRMMKN
WAERRRNAMR LLEELDILPE KMEQAMEELD EIIRAWADKY HQDGGIAVVL QTVFGEKED
