ID   HETR_TRIEI              Reviewed;         302 AA.
AC   Q93CE9; Q113Z8;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 2.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=DNA-binding transcriptional activator HetR {ECO:0000255|HAMAP-Rule:MF_00781};
DE            EC=3.4.21.- {ECO:0000255|HAMAP-Rule:MF_00781};
GN   Name=hetR {ECO:0000255|HAMAP-Rule:MF_00781}; OrderedLocusNames=Tery_1921;
OS   Trichodesmium erythraeum (strain IMS101).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales;
OC   Microcoleaceae; Trichodesmium.
OX   NCBI_TaxID=203124;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IMS101;
RX   PubMed=12151106; DOI=10.1016/s0167-4781(02)00399-8;
RA   Schiefer W., Schuetz K., Hachtel W., Happe T.;
RT   "Molecular cloning and characterization of hetR genes from filamentous
RT   cyanobacteria.";
RL   Biochim. Biophys. Acta 1577:139-143(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IMS101;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Kiss H., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Richardson P.;
RT   "Complete sequence of Trichodesmium erythraeum IMS101.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   DOES NOT FORM HETEROCYSTS.
RX   PubMed=11711677; DOI=10.1126/science.1064082;
RA   Berman-Frank I., Lundgren P., Chen Y.B., Kuepper H., Kolber Z., Bergman B.,
RA   Falkowski P.;
RT   "Segregation of nitrogen fixation and oxygenic photosynthesis in the marine
RT   cyanobacterium Trichodesmium.";
RL   Science 294:1534-1537(2001).
CC   -!- FUNCTION: Might be involved in temporal and/or spatial regulation of
CC       nitrogen fixation. Dimerization is required for DNA-binding. Has both a
CC       protease and a DNA-binding activity. {ECO:0000255|HAMAP-Rule:MF_00781}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000255|HAMAP-
CC       Rule:MF_00781}.
CC   -!- MISCELLANEOUS: This filamentous cyanobacterium does not make
CC       heterocysts, instead a combined temporal and spatial segregation of
CC       nitrogen fixation and oxygenic photosynthesis permits the cells to fix
CC       nitrogen for only a few hours during the photoperiod.
CC       {ECO:0000269|PubMed:11711677}.
CC   -!- SIMILARITY: Belongs to the peptidase S48 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00781}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABG51176.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF410432; AAL05045.1; -; Genomic_DNA.
DR   EMBL; CP000393; ABG51176.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q93CE9; -.
DR   SMR; Q93CE9; -.
DR   STRING; 203124.Tery_1921; -.
DR   MEROPS; S48.001; -.
DR   PRIDE; Q93CE9; -.
DR   EnsemblBacteria; ABG51176; ABG51176; Tery_1921.
DR   KEGG; ter:Tery_1921; -.
DR   eggNOG; ENOG502Z96Q; Bacteria.
DR   HOGENOM; CLU_926376_0_0_3; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043158; P:heterocyst differentiation; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1670; -; 1.
DR   Gene3D; 1.10.10.1680; -; 1.
DR   HAMAP; MF_00781; HetR; 1.
DR   InterPro; IPR040949; HetR_C.
DR   InterPro; IPR041936; HetR_DNA-bd_N.
DR   InterPro; IPR041935; HetR_flap.
DR   InterPro; IPR005319; Pept_S48_HetR.
DR   Pfam; PF18460; HetR_C; 1.
DR   Pfam; PF03574; Peptidase_S48; 1.
PE   3: Inferred from homology;
KW   Activator; Disulfide bond; DNA-binding; Hydrolase; Protease;
KW   Serine protease; Transcription; Transcription regulation.
FT   CHAIN           1..302
FT                   /note="DNA-binding transcriptional activator HetR"
FT                   /id="PRO_0000208483"
FT   ACT_SITE        153
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00781"
FT   DISULFID        49
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00781"
FT   CONFLICT        37
FT                   /note="G -> R (in Ref. 1; AAL05045)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   302 AA;  35339 MW;  784B94BFACB74AD8 CRC64;
     MMKTDTDLIN SLSPSAMDQI MLYLAFSAMR TSGHRHGAFL DAAATAAKCA IYMTYIEQGQ
     NLRMTGHLHH IEPKRVKVIV QEVEEALTKG KLLKMLGSQE PRYLIQFPYV WLEQYPWTPS
     RSRLPGNNLT TEEKRYIEGK LPSNMPDARL INSFQFMELI EFLHRRSQED FPPERRMPLS
     EALAEHIKRR LIYSGTVTKI DSPWGMPFYA LTRSSYSPEG QEERTYIMVE DTARYFRLMK
     DWAENNNTNK VMRILEEFDI SPDRFEQAKE DLDEIIRHWA DRYHESGGKQ MVVQMVFGLK
     DD