ID   HEXB_PSEO7              Reviewed;         773 AA.
AC   P49007;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Beta-hexosaminidase B;
DE            EC=3.2.1.52;
DE   AltName: Full=Beta-GlcNAcase;
DE   AltName: Full=Beta-N-acetylhexosaminidase;
DE            Short=Beta-NAHase;
DE   AltName: Full=N-acetyl-beta-glucosaminidase;
DE   Flags: Precursor;
GN   Name=nag096;
OS   Pseudoalteromonas piscicida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=43662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O-7;
RX   PubMed=7574618; DOI=10.1128/aem.61.2.804-806.1995;
RA   Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Kimura Y., Imada C.,
RA   Okami Y., Inamori Y.;
RT   "Molecular cloning of the gene which encodes beta-N-acetylglucosaminidase
RT   from a marine bacterium, Alteromonas sp. strain O-7.";
RL   Appl. Environ. Microbiol. 61:804-806(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC         residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family. {ECO:0000305}.
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DR   EMBL; D29665; BAA06136.1; -; Genomic_DNA.
DR   AlphaFoldDB; P49007; -.
DR   SMR; P49007; -.
DR   STRING; 43662.TW75_19265; -.
DR   CAZy; GH20; Glycoside Hydrolase Family 20.
DR   PRIDE; P49007; -.
DR   GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030247; F:polysaccharide binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.40.290; -; 1.
DR   Gene3D; 3.30.379.10; -; 1.
DR   InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR   InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR   InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR   InterPro; IPR004866; CHB/HEX_N_dom.
DR   InterPro; IPR015883; Glyco_hydro_20_cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR029018; Hex-like_dom2.
DR   InterPro; IPR015882; HEX_bac_N.
DR   PANTHER; PTHR22600; PTHR22600; 1.
DR   Pfam; PF03173; CHB_HEX; 1.
DR   Pfam; PF00728; Glyco_hydro_20; 1.
DR   Pfam; PF02838; Glyco_hydro_20b; 1.
DR   PRINTS; PR00738; GLHYDRLASE20.
DR   SMART; SM01081; CHB_HEX; 1.
DR   SUPFAM; SSF49384; SSF49384; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF55545; SSF55545; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..773
FT                   /note="Beta-hexosaminidase B"
FT                   /id="PRO_0000012015"
FT   ACT_SITE        531
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        389..397
FT                   /evidence="ECO:0000250"
FT   DISULFID        496..542
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   773 AA;  86760 MW;  DBB379885E004642 CRC64;
     MKFNRLMALL FGVSSPLYAL DQTAVNWLGQ NLDVKYTLLD TKPTTCPKAQ QKCYYSELSF
     SVRKENTKAN NDFAIFFSQL MPIYHVEGDN FAITHINGDI HKITPAAGFS GFSSAPTTVR
     FYTKDSQVTR SEVYAKLCCE RTRSLKLTPQ VIKSTQTQRD NDTGLDCNLN LTPFVTLNQL
     QTSSKDDTPW MGSEYLYQHQ VKPTLDAAIG LIPKPKQLTV LSDKRLNLAA GINLQLSGIS
     ADAIAMAQQR LNTLGVKSTK EGLVVNVAVK PNKQSSPHYQ LTVAENNISI QGNNSAAAFY
     ALQSLAGLLD INDLRIPMVD IIDTPRYDFR GLHVDVARNF RSKAFILQTI EQMAAYKLNK
     LHLHLADDEG WRLAIDGLDE LTSVGAYRCF DLTETRCLLP QLGAGNDKNA QVNGFYSAED
     YIEILRYAKA HHIEVLPSLD MPGHSRAAII AMEARYKKLM AQGKPEDAQK YRLVETADKT
     RYSSIQHYND NTLNVCIANT YTFIDKVLSE VKVLHDRAGV PLNTYHIGAD ETAVLWLESP
     ACKKLQASVK DFTNFNGYFI ERVAKLLDKK GIQVAGWSDG LGDVRAANMP ANIQSNGLGD
     IKRKRAPVAH RFANQGWQVV LSSPDVTYFD FPYQSHPEER GNHWASRAIE SKKMFEFMPD
     NLPAHAEIWK NTNNHAYIAN DSDSSLNKGV QFAGLQGHLW SEMLRSDAQA EYMLYPRLLA
     LAERAWHHAE WELPYQAGRI YSQSSGYFTA KLQAQREADW QRFVAILGNS RTT