ID   HEXR_PSEPK              Reviewed;         287 AA.
AC   Q88P32;
DT   26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=HTH-type transcriptional regulator HexR {ECO:0000305};
GN   Name=hexR {ECO:0000312|EMBL:AAN66646.1};
GN   OrderedLocusNames=PP_1021 {ECO:0000312|EMBL:AAN66646.1};
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2]
RP   FUNCTION AS A REPRESSOR.
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=18245293; DOI=10.1128/jb.01726-07;
RA   del Castillo T., Duque E., Ramos J.L.;
RT   "A set of activators and repressors control peripheral glucose pathways in
RT   Pseudomonas putida to yield a common central intermediate.";
RL   J. Bacteriol. 190:2331-2339(2008).
RN   [3]
RP   FUNCTION, DNA-BINDING, ACTIVITY REGULATION, AND SUBUNIT.
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=19506074; DOI=10.1074/jbc.m109.014555;
RA   Daddaoua A., Krell T., Ramos J.L.;
RT   "Regulation of glucose metabolism in Pseudomonas: the phosphorylative
RT   branch and Entner-Doudoroff enzymes are regulated by a repressor containing
RT   a sugar isomerase domain.";
RL   J. Biol. Chem. 284:21360-21368(2009).
CC   -!- FUNCTION: Involved in regulation of glucose metabolism. Transcriptional
CC       repressor of the gap-1 gene and of the edd-glk-gltR-2 and zwf-pgl-eda
CC       operons. Acts by binding directly to an inverted pseudopalindromic
CC       sequence in the promoter region. {ECO:0000269|PubMed:18245293,
CC       ECO:0000269|PubMed:19506074}.
CC   -!- ACTIVITY REGULATION: Binding of 2-keto-3-deoxy-6-phosphogluconate
CC       (KDPG) to HexR releases the repressor from its target sequences.
CC       {ECO:0000269|PubMed:19506074}.
CC   -!- SUBUNIT: Monomer in solution. Binds DNA as a dimer.
CC       {ECO:0000269|PubMed:19506074}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE015451; AAN66646.1; -; Genomic_DNA.
DR   RefSeq; NP_743182.3; NC_002947.4.
DR   RefSeq; WP_061405576.1; NC_002947.4.
DR   AlphaFoldDB; Q88P32; -.
DR   SMR; Q88P32; -.
DR   STRING; 160488.PP_1021; -.
DR   EnsemblBacteria; AAN66646; AAN66646; PP_1021.
DR   KEGG; ppu:PP_1021; -.
DR   PATRIC; fig|160488.4.peg.1084; -.
DR   eggNOG; COG1737; Bacteria.
DR   HOGENOM; CLU_055769_0_0_6; -.
DR   OMA; VPFIHRS; -.
DR   PhylomeDB; Q88P32; -.
DR   BioCyc; PPUT160488:G1G01-1094-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   CollecTF; EXPREG_000011f0; -.
DR   GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR   GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR   GO; GO:0001217; F:DNA-binding transcription repressor activity; IPI:CollecTF.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:CollecTF.
DR   GO; GO:1901135; P:carbohydrate derivative metabolic process; IEA:InterPro.
DR   CDD; cd05013; SIS_RpiR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR000281; HTH_RpiR.
DR   InterPro; IPR035472; RpiR-like_SIS.
DR   InterPro; IPR001347; SIS_dom.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF01418; HTH_6; 1.
DR   Pfam; PF01380; SIS; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53697; SSF53697; 1.
DR   PROSITE; PS51071; HTH_RPIR; 1.
DR   PROSITE; PS51464; SIS; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..287
FT                   /note="HTH-type transcriptional regulator HexR"
FT                   /id="PRO_0000431321"
FT   DOMAIN          2..78
FT                   /note="HTH rpiR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00390"
FT   DOMAIN          122..261
FT                   /note="SIS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00797"
FT   DNA_BIND        38..57
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00390"
SQ   SEQUENCE   287 AA;  31322 MW;  60AA0CC94A769586 CRC64;
     MRNLLEQIQG RLDELNKAER KVAEVILLNP QQATRFSIAA LAQAAKVSEP TVNRFCRSFG
     VSGYPELKLQ LAQSLASGAA YVSRAVEADD DPAAYTQKIF ASAIASLDSA CQQLDPQQVS
     RAVDMMIQAR QIHFFGLGAS APVALDAQHK FFRFNLAVSA HADVLMQRML ASVAHTGDLF
     VIISYTGRTR ELVEVARLAR ENGASVLGLT AAGSPLANAC SLSLHIPLPE DTDIYMPMTS
     RIIQLTVLDV LATGMTLRRG VDFQPHLRKI KESLNASRYP IEDDDLN