HEY1_CANLF
ID HEY1_CANLF Reviewed; 304 AA.
AC Q9TSZ2;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Hairy/enhancer-of-split related with YRPW motif protein 1;
DE AltName: Full=Hairy and enhancer of split-related protein 1;
DE Short=HESR-1;
GN Name=HEY1; Synonyms=HESR1; ORFNames=BC8;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND SUBCELLULAR LOCATION.
RC TISSUE=Thyroid;
RX PubMed=10964405; DOI=10.1006/abio.2000.4674;
RA Pichon B., Mercan D., Pouillon V., Christophe-Hobertus C., Christophe D.;
RT "A method for the large-scale cloning of nuclear proteins and nuclear
RT targeting sequences on a functional basis.";
RL Anal. Biochem. 284:231-239(2000).
CC -!- FUNCTION: Transcriptional repressor which binds preferentially to the
CC canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch
CC signaling required for cardiovascular development. Specifically
CC required for the Notch-induced endocardial epithelial to mesenchymal
CC transition, which is itself criticial for cardiac valve and septum
CC development. May be required in conjunction with HEY2 to specify
CC arterial cell fate or identity. Promotes maintenance of neuronal
CC precursor cells and glial versus neuronal fate specification. Represses
CC transcription by the cardiac transcriptional activators GATA4 and GATA6
CC and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.
CC {ECO:0000250|UniProtKB:Q9WV93, ECO:0000250|UniProtKB:Q9Y5J3}.
CC -!- SUBUNIT: Self-associates. Interacts with HES1 and HEYL. Interacts with
CC HDAC1, NCOR1 and SIN3A. Interacts with GATA4 and GATA6. Interacts with
CC CCDC89/BOIP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380,
CC ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:10964405}.
CC -!- SIMILARITY: Belongs to the HEY family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ388551; CAB65543.1; -; mRNA.
DR RefSeq; NP_001002953.1; NM_001002953.1.
DR AlphaFoldDB; Q9TSZ2; -.
DR SMR; Q9TSZ2; -.
DR ELM; Q9TSZ2; -.
DR STRING; 9615.ENSCAFP00000012336; -.
DR PaxDb; Q9TSZ2; -.
DR Ensembl; ENSCAFT00000013332; ENSCAFP00000012336; ENSCAFG00000008391.
DR Ensembl; ENSCAFT00030022614; ENSCAFP00030019729; ENSCAFG00030012192.
DR Ensembl; ENSCAFT00845052586; ENSCAFP00845041270; ENSCAFG00845029695.
DR GeneID; 403420; -.
DR KEGG; cfa:403420; -.
DR CTD; 23462; -.
DR VEuPathDB; HostDB:ENSCAFG00845029695; -.
DR VGNC; VGNC:54114; HEY1.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00940000157068; -.
DR InParanoid; Q9TSZ2; -.
DR OrthoDB; 1201152at2759; -.
DR Proteomes; UP000002254; Chromosome 29.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:Ensembl.
DR GO; GO:0001525; P:angiogenesis; IEA:Ensembl.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0072359; P:circulatory system development; IBA:GO_Central.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IEA:Ensembl.
DR GO; GO:2000820; P:negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation; IEA:Ensembl.
DR GO; GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IEA:Ensembl.
DR GO; GO:0007219; P:Notch signaling pathway; ISS:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..304
FT /note="Hairy/enhancer-of-split related with YRPW motif
FT protein 1"
FT /id="PRO_0000127216"
FT DOMAIN 49..104
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 122..158
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..117
FT /note="Transcriptional repression and interaction with
FT NCOR1 and SIN3A"
FT /evidence="ECO:0000250"
FT REGION 191..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 304 AA; 32472 MW; 191D5BD42970F412 CRC64;
MKRAHPDYSS SDSELDETVE VEKESADENG NLSSALGSMS PTTSSQILAR KRRRGIIEKR
RRDRINNSLS ELRRLVPSAF EKQGSAKLEK AEILQMTVDH LKMLHTAGGK GYFDAHALAM
DYRSLGFREC LAEVARYLSI IEGLDASDPL RVRLVSHLNN YASQREAASG AHAGLGHLPW
GSAFGHHPHV AHPLLLPQSG HGNTGTSASP TDPHHQGRLA AAHPEAPALR APPSGGLGPV
LPVVTSASKL SPPLLSSVAS LSAFPFSFGS FHLLSPNALS PSAPTQAANL GKPYRPWGTE
IGAF
