HEY1_XENTR
ID HEY1_XENTR Reviewed; 300 AA.
AC Q66KK8;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Hairy/enhancer-of-split related with YRPW motif protein 1;
GN Name=hey1; ORFNames=TEgg037i08.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Egg;
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tail bud;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Downstream effector of Notch signaling. Transcriptional
CC repressor which binds preferentially to the canonical E box sequence
CC 5'-CACGTG-3'. Acts as a suppressor of neurogenesis by antagonizing
CC proneural gene function. Functions during floorplate development. Plays
CC a role in pronephros formation in the inhibition of distal tubule and
CC duct cell fates and the promotion of glomus and proximal tubule
CC formation (By similarity). {ECO:0000250|UniProtKB:Q9I8A3,
CC ECO:0000250|UniProtKB:Q9WV93}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Binds DNA in the form of homodimer or more strongly as a
CC heterodimer with hes1/hairy1 or hes4/hairy2b. Also weakly interacts
CC with the bHLH proteins hes2, neurod1 and neurod4/ath3. Interacts (via
CC Orange domain) with ccdc89/boip (via C-terminus) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DOMAIN: The Orange domain, downstream sequence and the bHLH are
CC required for efficient heterodimerization with hes/hairy proteins, and
CC for transcriptional repressor activity. {ECO:0000250}.
CC -!- DOMAIN: The C-terminal YRPW motif is not required for transcriptional
CC repressor activity and is unable to recruit groucho. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HEY family. {ECO:0000305}.
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DR EMBL; CR760684; CAJ83693.1; -; mRNA.
DR EMBL; BC080349; AAH80349.1; -; mRNA.
DR RefSeq; NP_001007911.1; NM_001007910.2.
DR AlphaFoldDB; Q66KK8; -.
DR SMR; Q66KK8; -.
DR PaxDb; Q66KK8; -.
DR DNASU; 493293; -.
DR GeneID; 493293; -.
DR KEGG; xtr:493293; -.
DR CTD; 23462; -.
DR Xenbase; XB-GENE-486694; hey1.
DR eggNOG; KOG4304; Eukaryota.
DR HOGENOM; CLU_048294_2_0_1; -.
DR InParanoid; Q66KK8; -.
DR OrthoDB; 1201152at2759; -.
DR PhylomeDB; Q66KK8; -.
DR Proteomes; UP000008143; Chromosome 6.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000007864; Expressed in neurula embryo and 34 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0072359; P:circulatory system development; IBA:GO_Central.
DR GO; GO:0033504; P:floor plate development; ISS:UniProtKB.
DR GO; GO:0072013; P:glomus development; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007219; P:Notch signaling pathway; ISS:UniProtKB.
DR GO; GO:0048793; P:pronephros development; ISS:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0072082; P:specification of proximal tubule identity; ISS:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..300
FT /note="Hairy/enhancer-of-split related with YRPW motif
FT protein 1"
FT /id="PRO_0000286427"
FT DOMAIN 48..103
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 121..157
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 290..293
FT /note="YRPW motif"
FT COMPBIAS 27..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 300 AA; 32565 MW; 013406A12136DF11 CRC64;
MKRGHDYSSS DSELDENIEV EKESADENGN LSSAAGSMSP STSSQILARK RRRGIIEKRR
RDRINNSLSE LRRLVPSAFE KQGSAKLEKA EILQMTVDHL KMLHTAGGKG YFDAHALAMD
YRSLGFRECL AEVARYLSII EGMDTTDPLR VRLVSHLNNY ASQREAANTA HTGIGHIPWG
GTFAHHPHLS HPLLLAQTAH TSANSTSSST EAHHQNRLPG SPHAETSSLR VPPNGNIASV
LPVVASSKLS PPLLSSMASL SAFPFSFGSF HLLSPNSLSP TTPTPSGKPY RPWGTEIGAF
