HFA1E_ARATH
ID HFA1E_ARATH Reviewed; 468 AA.
AC Q9SCW5; Q0V855; Q9M8U0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Heat stress transcription factor A-1e;
DE Short=AtHsfA1e;
DE AltName: Full=AtHsf-06;
DE AltName: Full=Heat shock factor protein 2;
DE Short=HSF 2;
DE AltName: Full=Heat shock transcription factor 2;
DE Short=HSTF 2;
GN Name=HSFA1E; Synonyms=HSF06, HSF2; OrderedLocusNames=At3g02990;
GN ORFNames=F13E7.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Green siliques;
RA Schoeffl F., Praendl R.;
RT "De-repression of heat shock protein synthesis in transgenic plants.";
RL (In) Smallwood M.F., Calvert C.M., Bowles D.J. (eds.);
RL Plant responses to environmental stress, pp.65-73, BIOS Scientific
RL Publishers, Oxford (1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT "Arabidopsis and the heat stress transcription factor world: how many heat
RT stress transcription factors do we need?";
RL Cell Stress Chaperones 6:177-189(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
CC -!- FUNCTION: Transcriptional activator that specifically binds DNA
CC sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motif is a transcriptional activator
CC element. {ECO:0000269|PubMed:11599559}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ251865; CAB63800.1; -; mRNA.
DR EMBL; AC018363; AAF26960.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73888.1; -; Genomic_DNA.
DR EMBL; BT026365; ABH04472.1; -; mRNA.
DR RefSeq; NP_186949.1; NM_111169.5.
DR AlphaFoldDB; Q9SCW5; -.
DR SMR; Q9SCW5; -.
DR BioGRID; 6495; 10.
DR IntAct; Q9SCW5; 9.
DR STRING; 3702.AT3G02990.1; -.
DR iPTMnet; Q9SCW5; -.
DR PaxDb; Q9SCW5; -.
DR PRIDE; Q9SCW5; -.
DR ProteomicsDB; 230238; -.
DR EnsemblPlants; AT3G02990.1; AT3G02990.1; AT3G02990.
DR GeneID; 821162; -.
DR Gramene; AT3G02990.1; AT3G02990.1; AT3G02990.
DR KEGG; ath:AT3G02990; -.
DR Araport; AT3G02990; -.
DR TAIR; locus:2075447; AT3G02990.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_0_2_1; -.
DR InParanoid; Q9SCW5; -.
DR OMA; HNMSNPQ; -.
DR OrthoDB; 1154048at2759; -.
DR PhylomeDB; Q9SCW5; -.
DR PRO; PR:Q9SCW5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SCW5; baseline and differential.
DR Genevisible; Q9SCW5; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..468
FT /note="Heat stress transcription factor A-1e"
FT /id="PRO_0000124583"
FT DNA_BIND 21..115
FT /evidence="ECO:0000250"
FT REGION 133..199
FT /note="Hydrophobic repeat HR-A/B"
FT REGION 211..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 223..227
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 402..411
FT /note="AHA"
FT MOTIF 454..461
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT CONFLICT 68
FT /note="K -> N (in Ref. 1; CAB63800)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 468 AA; 51963 MW; 7ED5431F4F3ADDB5 CRC64;
MGTVCESVAT AKSSTAVMSS IPPFLSKTYD MVDDPLTDDV VSWSSGNNSF VVWNVPEFAK
QFLPKYFKHN NFSSFVRQLN TYGFRKVDPD RWEFANEGFL RGQKQILKSI VRRKPAQVQP
PQQPQVQHSS VGACVEVGKF GLEEEVERLQ RDKNVLMQEL VRLRQQQQVT EHHLQNVGQK
VHVMEQRQQQ MMSFLAKAVQ SPGFLNQFSQ QSNEANQHIS ESNKKRRLPV EDQMNSGSHG
VNGLSRQIVR YQSSMNDATN TMLQQIQQMS NAPSHESLSS NNGSFLLGDV PNSNISDNGS
SSNGSPEVTL ADVSSIPAGF YPAMKYHEPC ETNQVMETNL PFSQGDLLPP TQGAAASGSS
SSDLVGCETD NGECLDPIMA VLDGALELEA DTLNELLPEV QDSFWEQFIG ESPVIGETDE
LISGSVENEL ILEQLELQST LSNVWSKNQQ MNHLTEQMGL LTSDALRK
