ID   HFA2B_ORYSJ             Reviewed;         372 AA.
AC   Q6VBB2; A0A0P0X300; Q7XBH0;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Heat stress transcription factor A-2b;
DE   AltName: Full=Heat stress transcription factor 18;
DE            Short=OsHsf-18;
DE   AltName: Full=Heat stress transcription factor 5;
DE            Short=rHsf5;
GN   Name=HSFA2B; Synonyms=HS1, HSF18, HSF5;
GN   OrderedLocusNames=Os07g0178600, LOC_Os07g08140;
GN   ORFNames=OJ1361_E02.115, OsJ_022374;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Quanhong Y., Rihe P., Aisheng X.;
RT   "Isolation a putative heat stress transcription factor of Oryza sativa.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Yao Q.-H., Peng R.-H., Xiong A.-S.;
RT   "Isolation rice heat shock factor by modified yeast one-hybrid system
RT   method.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15625403; DOI=10.1007/bf02712120;
RA   Baniwal S.K., Bharti K., Chan K.Y., Fauth M., Ganguli A., Kotak S.,
RA   Mishra S.K., Nover L., Port M., Scharf K.-D., Tripp J., Weber C.,
RA   Zielinski D., von Koskull-Doering P.;
RT   "Heat stress response in plants: a complex game with chaperones and more
RT   than twenty heat stress transcription factors.";
RL   J. Biosci. 29:471-487(2004).
RN   [8]
RP   GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX   PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA   Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT   "Genome-wide analysis of heat shock transcription factor families in rice
RT   and Arabidopsis.";
RL   J. Genet. Genomics 35:105-118(2008).
RN   [9]
RP   INDUCTION.
RX   PubMed=18064488; DOI=10.1007/s00425-007-0670-4;
RA   Yokotani N., Ichikawa T., Kondou Y., Matsui M., Hirochika H., Iwabuchi M.,
RA   Oda K.;
RT   "Expression of rice heat stress transcription factor OsHsfA2e enhances
RT   tolerance to environmental stresses in transgenic Arabidopsis.";
RL   Planta 227:957-967(2008).
CC   -!- FUNCTION: Transcriptional regulator that specifically binds DNA of heat
CC       shock promoter elements (HSE). {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC   -!- INDUCTION: By heat stress. {ECO:0000269|PubMed:18064488}.
CC   -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC       oligomerization domain. AHA motifs are transcriptional activator
CC       elements. {ECO:0000269|PubMed:18407058}.
CC   -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY323488; AAP92754.1; -; mRNA.
DR   EMBL; AY344487; AAQ23059.1; -; mRNA.
DR   EMBL; AP003826; BAD30327.1; -; Genomic_DNA.
DR   EMBL; AP008213; BAF20942.1; -; Genomic_DNA.
DR   EMBL; AP014963; BAT00300.1; -; Genomic_DNA.
DR   EMBL; CM000144; EAZ38891.1; -; Genomic_DNA.
DR   RefSeq; XP_015645272.1; XM_015789786.1.
DR   AlphaFoldDB; Q6VBB2; -.
DR   SMR; Q6VBB2; -.
DR   STRING; 4530.OS07T0178600-01; -.
DR   PaxDb; Q6VBB2; -.
DR   PRIDE; Q6VBB2; -.
DR   EnsemblPlants; Os07t0178600-01; Os07t0178600-01; Os07g0178600.
DR   GeneID; 4342550; -.
DR   Gramene; Os07t0178600-01; Os07t0178600-01; Os07g0178600.
DR   KEGG; osa:4342550; -.
DR   eggNOG; KOG0627; Eukaryota.
DR   HOGENOM; CLU_030308_1_0_1; -.
DR   InParanoid; Q6VBB2; -.
DR   OMA; NFWEQLL; -.
DR   OrthoDB; 1154048at2759; -.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000007752; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   Genevisible; Q6VBB2; OS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000232; HSF_DNA-bd.
DR   InterPro; IPR027725; HSF_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10015; PTHR10015; 1.
DR   Pfam; PF00447; HSF_DNA-bind; 1.
DR   PRINTS; PR00056; HSFDOMAIN.
DR   SMART; SM00415; HSF; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00434; HSF_DOMAIN; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein;
KW   Reference proteome; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..372
FT                   /note="Heat stress transcription factor A-2b"
FT                   /id="PRO_0000350822"
FT   REGION          173..223
FT                   /note="Hydrophobic repeat HR-A/B"
FT   REGION          247..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          165..252
FT                   /evidence="ECO:0000255"
FT   MOTIF           238..253
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           328..337
FT                   /note="AHA"
FT   MOTIF           356..363
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        37
FT                   /note="V -> L (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        40
FT                   /note="R -> G (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        136
FT                   /note="L -> F (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        155
FT                   /note="L -> F (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        213
FT                   /note="Q -> K (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        216
FT                   /note="M -> I (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="E -> Q (in Ref. 1; AAP92754)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   372 AA;  41524 MW;  1E3E75A957FD098D CRC64;
     MDDPMLNAVK EEESHGDGGG LEVVAGEDGA AAVAAGVAPR PMEGLHDAGP PPFLTKTYDM
     VDDAGTDAAV SWSATSNSFV VWDPHAFATV LLPRFFKHNN FSSFVRQLNT YGFRKVDPDR
     WEFANENFLR GQRHLLKNIK RRKPPSHTAS NQQSLGPYLE VGHFGYDAEI DRLKRDKQLL
     MAEVVKLRQE QQNTKANLKA MEDRLQGTEQ RQQQMMAFLA RVMKNPEFLK QLMSQNEMRK
     ELQDAISKKR RRRIDQGPEV DDVGTSSSIE QESPALFDPQ ESVEFLIDGI PSDLENSAMD
     AGGLVEPQDF DVGASEQQQI GPQGELNDNF WEELLNEGLV GEENDNPVVE DDMNVLSEKM
     GYLNSNGPTA GE