HFA2D_ORYSJ
ID HFA2D_ORYSJ Reviewed; 359 AA.
AC Q8H7Y6;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Heat stress transcription factor A-2d;
DE AltName: Full=Heat stress transcription factor 7;
DE Short=rHsf7;
DE AltName: Full=Heat stress transcription factor 8;
DE Short=OsHsf-08;
GN Name=HSFA2D; Synonyms=HSF08, HSF7;
GN OrderedLocusNames=Os03g0161900, LOC_Os03g06630;
GN ORFNames=OJ1607A12.12, OsJ_009160;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Yao Q.-H., Peng R.-H., Xiong A.-S.;
RT "Isolation rice heat shock factor by modified yeast one-hybrid system
RT method.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15625403; DOI=10.1007/bf02712120;
RA Baniwal S.K., Bharti K., Chan K.Y., Fauth M., Ganguli A., Kotak S.,
RA Mishra S.K., Nover L., Port M., Scharf K.-D., Tripp J., Weber C.,
RA Zielinski D., von Koskull-Doering P.;
RT "Heat stress response in plants: a complex game with chaperones and more
RT than twenty heat stress transcription factors.";
RL J. Biosci. 29:471-487(2004).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
RN [9]
RP INDUCTION.
RX PubMed=18064488; DOI=10.1007/s00425-007-0670-4;
RA Yokotani N., Ichikawa T., Kondou Y., Matsui M., Hirochika H., Iwabuchi M.,
RA Oda K.;
RT "Expression of rice heat stress transcription factor OsHsfA2e enhances
RT tolerance to environmental stresses in transgenic Arabidopsis.";
RL Planta 227:957-967(2008).
CC -!- FUNCTION: Transcriptional regulator that specifically binds DNA of heat
CC shock promoter elements (HSE). {ECO:0000250}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8H7Y6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8H7Y6-2; Sequence=VSP_035445;
CC -!- INDUCTION: By heat stress. {ECO:0000269|PubMed:18064488}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motifs are transcriptional activator
CC elements. {ECO:0000269|PubMed:18407058}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
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DR EMBL; AY344489; AAQ23061.1; -; mRNA.
DR EMBL; AC105729; AAN06862.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94118.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10961.1; -; Genomic_DNA.
DR EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000140; EAZ25677.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8H7Y6; -.
DR SMR; Q8H7Y6; -.
DR IntAct; Q8H7Y6; 1.
DR MINT; Q8H7Y6; -.
DR STRING; 39947.Q8H7Y6; -.
DR PRIDE; Q8H7Y6; -.
DR EnsemblPlants; Os03t0161900-02; Os03t0161900-02; Os03g0161900. [Q8H7Y6-2]
DR Gramene; Os03t0161900-02; Os03t0161900-02; Os03g0161900. [Q8H7Y6-2]
DR InParanoid; Q8H7Y6; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..359
FT /note="Heat stress transcription factor A-2d"
FT /id="PRO_0000350824"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..208
FT /note="Hydrophobic repeat HR-A/B"
FT REGION 290..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 150..202
FT /evidence="ECO:0000255"
FT MOTIF 233..237
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 279..286
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT MOTIF 315..324
FT /note="AHA"
FT COMPBIAS 292..312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 96
FT /note="Y -> YFLVRTNYLNKRSHFYSLRFQ (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_035445"
SQ SEQUENCE 359 AA; 41135 MW; CCB81A4DA9981EE6 CRC64;
MEKMMPGMVK EEWPPSSPEE GEAPRPMEGL HEVGPPPFLT KTFDLVADPA TDGVVSWGRA
GSSFVVWDPH VFAAVFLPRF FKHNNFSSFV RQLNTYGFRK IDPDRWEFAN DGFLRGQRHL
LKMIKRRRPL SYLPGSQQAL GTCLEVGQFG LDEEIDRLKR DKNILLAEVV KLRHKQQSTK
ANMRAMEERL QHAEQKQVQM MGFLARAMQN PDFFHQLIHQ QDKMKGLEDT FSKKRTRSID
IVPFLNPGEV SQGDQLESTL LFDPRPFAEL NDEPAKSELE NLALNIQGLG KGKQDVNRTR
NQPRNQASNE TELTDDFWEE LLNEGARDDA GIPGMERRRP RYVDALAQKL GYLSNSSQK
