ID   HFA4C_ARATH             Reviewed;         345 AA.
AC   Q9FK72;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 153.
DE   RecName: Full=Heat stress transcription factor A-4c;
DE            Short=AtHsfA4c;
DE   AltName: Full=AtHsf-20;
DE   AltName: Full=Protein ROOT HANDEDNESS 1;
GN   Name=HSFA4C; Synonyms=HSF20, RHA1; OrderedLocusNames=At5g45710;
GN   ORFNames=MRA19.11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Wassilewskija;
RA   Piconese S., Faggiano M., Rosi C., Migliaccio F.;
RT   "The AtRHA1 gene of Arabidopsis: structure and possible functions.";
RL   (In) Proceedings of the 13th international conference on Arabidopsis
RL   research, abstract#4-31, Seville (2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX   PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA   Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT   "Arabidopsis and the heat stress transcription factor world: how many heat
RT   stress transcription factors do we need?";
RL   Cell Stress Chaperones 6:177-189(2001).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18381353; DOI=10.1093/jxb/ern047;
RA   Fortunati A., Piconese S., Tassone P., Ferrari S., Migliaccio F.;
RT   "A new mutant of Arabidopsis disturbed in its roots, right-handed slanting,
RT   and gravitropism defines a gene that encodes a heat-shock factor.";
RL   J. Exp. Bot. 59:1363-1374(2008).
RN   [8]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA   Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT   "Genome-wide analysis of heat shock transcription factor families in rice
RT   and Arabidopsis.";
RL   J. Genet. Genomics 35:105-118(2008).
CC   -!- FUNCTION: Transcriptional activator that specifically binds DNA
CC       sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC       May be involved in general response to auxin.
CC       {ECO:0000269|PubMed:18381353}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9FK72-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Expressed in roots, seedlings and at lower levels
CC       in leaves. {ECO:0000269|PubMed:18381353}.
CC   -!- INDUCTION: By heat and cold stresses and the herbicide 2,4-
CC       dichlorophenoxyacetic acid (2,4-D, auxin analog).
CC       {ECO:0000269|PubMed:18381353}.
CC   -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC       oligomerization domain. AHA motifs are transcriptional activator
CC       elements. {ECO:0000269|PubMed:11599559}.
CC   -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Plants display minimal right-handed slanting in
CC       roots, reduced gravitropic response, reduced number of lateral roots,
CC       reduced size of shoot and root in the seedlings and increased
CC       resistance to 2,4-D. {ECO:0000269|PubMed:18381353}.
CC   -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY350739; AAQ54332.1; -; Genomic_DNA.
DR   EMBL; AB012245; BAB09213.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95287.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM70169.1; -; Genomic_DNA.
DR   EMBL; BT015049; AAT71921.1; -; mRNA.
DR   EMBL; BT015843; AAU94406.1; -; mRNA.
DR   EMBL; AK229058; BAF00940.1; -; mRNA.
DR   RefSeq; NP_001331800.1; NM_001344637.1. [Q9FK72-1]
DR   RefSeq; NP_199383.1; NM_123938.4. [Q9FK72-1]
DR   AlphaFoldDB; Q9FK72; -.
DR   SMR; Q9FK72; -.
DR   BioGRID; 19859; 9.
DR   IntAct; Q9FK72; 9.
DR   STRING; 3702.AT5G45710.1; -.
DR   PaxDb; Q9FK72; -.
DR   PRIDE; Q9FK72; -.
DR   ProteomicsDB; 230274; -. [Q9FK72-1]
DR   EnsemblPlants; AT5G45710.1; AT5G45710.1; AT5G45710. [Q9FK72-1]
DR   EnsemblPlants; AT5G45710.3; AT5G45710.3; AT5G45710. [Q9FK72-1]
DR   GeneID; 834610; -.
DR   Gramene; AT5G45710.1; AT5G45710.1; AT5G45710. [Q9FK72-1]
DR   Gramene; AT5G45710.3; AT5G45710.3; AT5G45710. [Q9FK72-1]
DR   KEGG; ath:AT5G45710; -.
DR   Araport; AT5G45710; -.
DR   TAIR; locus:2172003; AT5G45710.
DR   eggNOG; KOG0627; Eukaryota.
DR   HOGENOM; CLU_030308_0_0_1; -.
DR   InParanoid; Q9FK72; -.
DR   OMA; MDENNGG; -.
DR   PhylomeDB; Q9FK72; -.
DR   PRO; PR:Q9FK72; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FK72; baseline and differential.
DR   Genevisible; Q9FK72; AT.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0048530; P:fruit morphogenesis; IMP:TAIR.
DR   GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR   GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR   GO; GO:0009408; P:response to heat; IEP:TAIR.
DR   GO; GO:0048364; P:root development; IMP:TAIR.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000232; HSF_DNA-bd.
DR   InterPro; IPR027725; HSF_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10015; PTHR10015; 1.
DR   Pfam; PF00447; HSF_DNA-bind; 1.
DR   PRINTS; PR00056; HSFDOMAIN.
DR   SMART; SM00415; HSF; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00434; HSF_DOMAIN; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW   Reference proteome; Repeat; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..345
FT                   /note="Heat stress transcription factor A-4c"
FT                   /id="PRO_0000270805"
FT   DNA_BIND        11..105
FT                   /evidence="ECO:0000250"
FT   REGION          119..185
FT                   /note="Hydrophobic repeat HR-A/B"
FT   REGION          240..329
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           199..203
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           226..235
FT                   /note="AHA1"
FT   MOTIF           289..298
FT                   /note="AHA2"
FT   COMPBIAS        240..273
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   345 AA;  39648 MW;  DFB628DDEF77F902 CRC64;
     MDENNGGSSS LPPFLTKTYE MVDDSSSDSV VAWSENNKSF IVKNPAEFSR DLLPRFFKHK
     NFSSFIRQLN TYGFRKVDPE KWEFLNDDFV RGRPYLMKNI HRRKPVHSHS LVNLQAQNPL
     TESERRSMED QIERLKNEKE GLLAELQNQE QERKEFELQV TTLKDRLQHM EQHQKSIVAY
     VSQVLGKPGL SLNLENHERR KRRFQENSLP PSSSHIEQVE KLESSLTFWE NLVSESCEKS
     GLQSSSMDHD AAESSLSIGD TRPKSSKIDM NSEPPVTVTA PAPKTGVNDD FWEQCLTENP
     GSTEQQEVQS ERRDVGNDNN GNKIGNQRTY WWNSGNVNNI TEKAS