ID   HFA4D_ORYSJ             Reviewed;         459 AA.
AC   Q93VB5; A0PIP5; A0SH76; B7EQE5; Q93WD1;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Heat stress transcription factor A-4d;
DE   AltName: Full=Heat stress transcription factor 10;
DE            Short=rHsf10;
DE   AltName: Full=Heat stress transcription factor 15;
DE            Short=OsHsf-15;
DE   AltName: Full=Protein SPOTTED LEAF 7;
GN   Name=HSFA4D; Synonyms=HSF10, HSF15, SP17, SPL7;
GN   OrderedLocusNames=Os05g0530400, LOC_Os05g45410;
GN   ORFNames=OJ1131_E09.10, OsJ_018517,
GN   OsJ_19305 {ECO:0000312|EMBL:EEE64456.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE, INDUCTION,
RP   AND MUTAGENESIS OF TRP-40.
RC   STRAIN=cv. Nipponbare, and cv. Nohrin 8; TISSUE=Leaf;
RX   PubMed=12032317; DOI=10.1073/pnas.112209199;
RA   Yamanouchi U., Yano M., Lin H., Ashikari M., Yamada K.;
RT   "A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor
RT   protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:7530-7535(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Yao Q.-H., Peng R.-H., Xiong A.-S.;
RT   "Isolation rice heat shock factor by modified yeast one-hybrid system
RT   method.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-415 AND 109-415.
RX   PubMed=17112320; DOI=10.1371/journal.pgen.0020199;
RA   Tang T., Lu J., Huang J., He J., McCouch S.R., Shen Y., Kai Z.,
RA   Purugganan M.D., Shi S., Wu C.-I.;
RT   "Genomic variation in rice: genesis of highly polymorphic linkage blocks
RT   during domestication.";
RL   PLoS Genet. 2:E199-E199(2006).
RN   [10]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15625403; DOI=10.1007/bf02712120;
RA   Baniwal S.K., Bharti K., Chan K.Y., Fauth M., Ganguli A., Kotak S.,
RA   Mishra S.K., Nover L., Port M., Scharf K.-D., Tripp J., Weber C.,
RA   Zielinski D., von Koskull-Doering P.;
RT   "Heat stress response in plants: a complex game with chaperones and more
RT   than twenty heat stress transcription factors.";
RL   J. Biosci. 29:471-487(2004).
RN   [11]
RP   GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX   PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA   Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT   "Genome-wide analysis of heat shock transcription factor families in rice
RT   and Arabidopsis.";
RL   J. Genet. Genomics 35:105-118(2008).
CC   -!- FUNCTION: Transcriptional regulator that specifically binds DNA of heat
CC       shock promoter elements (HSE). {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Hardly expressed in young leaves at 14 days after
CC       sowing (DAS). Expression detected in adult leaves at 28 DAS and
CC       increases with growing stage. {ECO:0000269|PubMed:12032317}.
CC   -!- INDUCTION: By heat stress. {ECO:0000269|PubMed:12032317}.
CC   -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC       oligomerization domain. AHA motifs are transcriptional activator
CC       elements. {ECO:0000269|PubMed:18407058}.
CC   -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB050095; BAB71737.1; -; Genomic_DNA.
DR   EMBL; AB050096; BAB71738.1; -; mRNA.
DR   EMBL; AB050097; BAB71739.1; -; Genomic_DNA.
DR   EMBL; AB050098; BAB71740.1; -; mRNA.
DR   EMBL; AY344492; AAQ23064.1; -; mRNA.
DR   EMBL; AC111015; AAS72351.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF18041.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS95027.1; -; Genomic_DNA.
DR   EMBL; CM000142; EAZ35034.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE64456.1; -; Genomic_DNA.
DR   EMBL; AK100412; BAG94592.1; -; mRNA.
DR   EMBL; AY885953; AAY33578.1; -; Genomic_DNA.
DR   EMBL; AY885954; AAY33579.1; -; Genomic_DNA.
DR   EMBL; AY885955; AAY33580.1; -; Genomic_DNA.
DR   EMBL; AY885956; AAY33581.1; -; Genomic_DNA.
DR   EMBL; AY885957; AAY33582.1; -; Genomic_DNA.
DR   EMBL; AY885958; AAY33583.1; -; Genomic_DNA.
DR   EMBL; AY885959; AAY33584.1; -; Genomic_DNA.
DR   EMBL; DQ374867; ABD15811.1; -; Genomic_DNA.
DR   EMBL; DQ374868; ABD15812.1; -; Genomic_DNA.
DR   EMBL; DQ374869; ABD15813.1; -; Genomic_DNA.
DR   EMBL; DQ374870; ABD15814.1; -; Genomic_DNA.
DR   RefSeq; XP_015639851.1; XM_015784365.1.
DR   AlphaFoldDB; Q93VB5; -.
DR   SMR; Q93VB5; -.
DR   STRING; 4530.OS05T0530400-01; -.
DR   PaxDb; Q93VB5; -.
DR   PRIDE; Q93VB5; -.
DR   EnsemblPlants; Os05t0530400-01; Os05t0530400-01; Os05g0530400.
DR   GeneID; 4339409; -.
DR   Gramene; Os05t0530400-01; Os05t0530400-01; Os05g0530400.
DR   KEGG; osa:4339409; -.
DR   eggNOG; KOG0627; Eukaryota.
DR   HOGENOM; CLU_030308_0_0_1; -.
DR   InParanoid; Q93VB5; -.
DR   OMA; QRAGNYA; -.
DR   OrthoDB; 1154048at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   Genevisible; Q93VB5; OS.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000232; HSF_DNA-bd.
DR   InterPro; IPR027725; HSF_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10015; PTHR10015; 1.
DR   Pfam; PF00447; HSF_DNA-bind; 1.
DR   PRINTS; PR00056; HSFDOMAIN.
DR   SMART; SM00415; HSF; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00434; HSF_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW   Stress response; Transcription; Transcription regulation.
FT   CHAIN           1..459
FT                   /note="Heat stress transcription factor A-4d"
FT                   /id="PRO_0000350828"
FT   REGION          141..191
FT                   /note="Hydrophobic repeat HR-A/B"
FT   REGION          364..398
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          127..189
FT                   /evidence="ECO:0000255"
FT   MOTIF           209..213
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           397..406
FT                   /note="AHA"
FT   COMPBIAS        365..393
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         40
FT                   /note="W->C: In spl7; lesion mimic phenotype."
FT                   /evidence="ECO:0000269|PubMed:12032317"
SQ   SEQUENCE   459 AA;  51164 MW;  D7B8F294DB6FFB13 CRC64;
     MESSNLGGGG GGGGGGGPPP FLIKTYEMVE DAATNHVVSW GPGGASFVVW NPLDFSRDLL
     PKYFKHNNFS SFIRQLNTYG FRKIDPERWE FANEDFIRGH THLLKNIHRR KPVHSHSLQN
     QINGPLAESE RRELEEEINR LKYEKSILVA DLQRQNQQQY VINWQMQAME GRLVAMEQRQ
     KNIVASLCEM LQRRGGAVSS SLLESDHFSK KRRVPKMDLF VDDCAAGEEQ KVFQFQGIGT
     DAPAMPPVLP VTNGEAFDRV ELSLVSLEKL FQRANDACTA AEEMYSHGHG GTEPSTAICP
     EEMNTAPMET GIDLQLPASL HPSSPNTGNA HLHLSTELTE SPGFVQSPEL PMAEIREDIH
     VTRYPTQADV NSEIASSTDT SQDGTSETEA SHGPTNDVFW ERFLTETPRS CLDESERQES
     PKDDVKAELG CNGFHHREKV DQITEQMGHL ASAEQTLHT