HFA7A_ARATH
ID HFA7A_ARATH Reviewed; 272 AA.
AC Q9SV12; C0Z2D2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Heat stress transcription factor A-7a;
DE Short=AtHsfA7a;
DE AltName: Full=AtHsf-09;
GN Name=HSFA7A; Synonyms=HSF09; OrderedLocusNames=At3g51910;
GN ORFNames=F4F15.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT "Arabidopsis and the heat stress transcription factor world: how many heat
RT stress transcription factors do we need?";
RL Cell Stress Chaperones 6:177-189(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
CC -!- FUNCTION: Transcriptional activator that specifically binds DNA
CC sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9SV12; Q9LV52: HSFC1; NbExp=4; IntAct=EBI-15200882, EBI-4457746;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SV12-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SV12-2; Sequence=VSP_037988, VSP_037989;
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motif is a transcriptional activator
CC element. {ECO:0000269|PubMed:11599559}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL049711; CAB41311.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78861.1; -; Genomic_DNA.
DR EMBL; AK318746; BAH56861.1; -; mRNA.
DR PIR; T49070; T49070.
DR RefSeq; NP_190759.1; NM_115050.2. [Q9SV12-1]
DR AlphaFoldDB; Q9SV12; -.
DR SMR; Q9SV12; -.
DR BioGRID; 9672; 6.
DR IntAct; Q9SV12; 6.
DR STRING; 3702.AT3G51910.1; -.
DR PaxDb; Q9SV12; -.
DR PRIDE; Q9SV12; -.
DR ProteomicsDB; 230275; -. [Q9SV12-1]
DR EnsemblPlants; AT3G51910.1; AT3G51910.1; AT3G51910. [Q9SV12-1]
DR GeneID; 824354; -.
DR Gramene; AT3G51910.1; AT3G51910.1; AT3G51910. [Q9SV12-1]
DR KEGG; ath:AT3G51910; -.
DR Araport; AT3G51910; -.
DR TAIR; locus:2083690; AT3G51910.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_1_0_1; -.
DR InParanoid; Q9SV12; -.
DR OMA; QSTKANM; -.
DR OrthoDB; 1154048at2759; -.
DR PhylomeDB; Q9SV12; -.
DR PRO; PR:Q9SV12; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SV12; baseline and differential.
DR Genevisible; Q9SV12; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0032268; P:regulation of cellular protein metabolic process; IEP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IEP:TAIR.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..272
FT /note="Heat stress transcription factor A-7a"
FT /id="PRO_0000270809"
FT DNA_BIND 27..121
FT /evidence="ECO:0000250"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..186
FT /note="Hydrophobic repeat HR-A/B"
FT REGION 203..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 201..216
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 256..265
FT /note="AHA"
FT COMPBIAS 7..23
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..82
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_037988"
FT VAR_SEQ 83..88
FT /note="RQLNTY -> MLKKLQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_037989"
FT CONFLICT 184
FT /note="R -> G (in Ref. 3; BAH56861)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 272 AA; 31776 MW; D17413E6447DF27F CRC64;
MMNPFLPEGC DPPPPPQPME GLHENAPPPF LTKTFEMVDD PNTDHIVSWN RGGTSFVVWD
LHSFSTILLP RHFKHSNFSS FIRQLNTYGF RKIEAERWEF ANEEFLLGQR QLLKNIKRRN
PFTPSSSPSH DACNELRREK QVLMMEIVSL RQQQQTTKSY IKAMEQRIEG TERKQRQMMS
FLARAMQSPS FLHQLLKQRD KKIKELEDNE SAKRKRGSSS MSELEVLALE MQGHGKQRNM
LEEEDHQLVV ERELDDGFWE ELLSDESLAS TS
