HFA7B_ARATH
ID HFA7B_ARATH Reviewed; 282 AA.
AC Q9M1V5; A0MF41;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Heat stress transcription factor A-7b;
DE Short=AtHsfA7b;
DE AltName: Full=AtHsf-10;
GN Name=HSFA7B; Synonyms=HSF10; OrderedLocusNames=At3g63350;
GN ORFNames=F16M2.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT "Arabidopsis and the heat stress transcription factor world: how many heat
RT stress transcription factors do we need?";
RL Cell Stress Chaperones 6:177-189(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
CC -!- FUNCTION: Transcriptional activator that specifically binds DNA
CC sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motif is a transcriptional activator
CC element. {ECO:0000269|PubMed:11599559}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28614.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL138648; CAB86436.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80470.1; -; Genomic_DNA.
DR EMBL; DQ446784; ABE66034.1; -; mRNA.
DR EMBL; DQ653166; ABK28614.1; ALT_SEQ; mRNA.
DR PIR; T48124; T48124.
DR RefSeq; NP_191894.1; NM_116200.2.
DR AlphaFoldDB; Q9M1V5; -.
DR SMR; Q9M1V5; -.
DR STRING; 3702.AT3G63350.1; -.
DR PaxDb; Q9M1V5; -.
DR PRIDE; Q9M1V5; -.
DR EnsemblPlants; AT3G63350.1; AT3G63350.1; AT3G63350.
DR GeneID; 825510; -.
DR Gramene; AT3G63350.1; AT3G63350.1; AT3G63350.
DR KEGG; ath:AT3G63350; -.
DR Araport; AT3G63350; -.
DR TAIR; locus:2077249; AT3G63350.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_1_0_1; -.
DR InParanoid; Q9M1V5; -.
DR OMA; YCERGFR; -.
DR OrthoDB; 1154048at2759; -.
DR PhylomeDB; Q9M1V5; -.
DR PRO; PR:Q9M1V5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1V5; baseline and differential.
DR Genevisible; Q9M1V5; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IEP:TAIR.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..282
FT /note="Heat stress transcription factor A-7b"
FT /id="PRO_0000270810"
FT DNA_BIND 26..120
FT /evidence="ECO:0000250"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 117..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 137..196
FT /note="Hydrophobic repeat HR-A/B"
FT MOTIF 208..212
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 232..240
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT MOTIF 259..268
FT /note="AHA"
FT COMPBIAS 117..135
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 282 AA; 32624 MW; 5F1775E3DC78ECFC CRC64;
MDPSSSSRAR SMPPPVPMEG LQEAGPSPFL TKTFEMVGDP NTNHIVSWNR GGISFVVWDP
HSFSATILPL YFKHNNFSSF VRQLNTYGFR KIEAERWEFM NEGFLMGQRD LLKSIKRRTS
SSSPPSLNYS QSQPEAHDPG VELPQLREER HVLMMEISTL RQEEQRARGY VQAMEQRING
AEKKQRHMMS FLRRAVENPS LLQQIFEQKR DREEAAMIDQ AGLIKMEEVE HLSELEALAL
EMQGYGRQRT DGVERELDDG FWEELLMNNE NSDEEEANVK QD
