HFB2A_ARATH
ID HFB2A_ARATH Reviewed; 299 AA.
AC Q9SCW4; Q0WMX0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 156.
DE RecName: Full=Heat stress transcription factor B-2a;
DE Short=AtHsfB2a;
DE AltName: Full=AtHsf-22;
DE AltName: Full=Heat shock factor protein 6;
DE Short=HSF 6;
DE AltName: Full=Heat shock transcription factor 6;
DE Short=HSTF 6;
GN Name=HSFB2A; Synonyms=HSF22, HSF6; OrderedLocusNames=At5g62020;
GN ORFNames=MTG10.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Green siliques;
RA Schoeffl F., Praendl R.;
RT "De-repression of heat shock protein synthesis in transgenic plants.";
RL (In) Smallwood M.F., Calvert C.M., Bowles D.J. (eds.);
RL Plant responses to environmental stress, pp.65-73, BIOS Scientific
RL Publishers, Oxford (1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT "Arabidopsis and the heat stress transcription factor world: how many heat
RT stress transcription factors do we need?";
RL Cell Stress Chaperones 6:177-189(2001).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
CC -!- FUNCTION: Transcriptional regulator that specifically binds DNA
CC sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class B subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ251867; CAB63802.1; -; mRNA.
DR EMBL; AB016880; BAB10163.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97552.1; -; Genomic_DNA.
DR EMBL; BT008542; AAP40369.1; -; mRNA.
DR EMBL; BT008658; AAP40470.1; -; mRNA.
DR EMBL; AK229690; BAF01530.1; -; mRNA.
DR RefSeq; NP_201008.2; NM_125595.4.
DR AlphaFoldDB; Q9SCW4; -.
DR SMR; Q9SCW4; -.
DR STRING; 3702.AT5G62020.1; -.
DR iPTMnet; Q9SCW4; -.
DR PaxDb; Q9SCW4; -.
DR PRIDE; Q9SCW4; -.
DR ProteomicsDB; 230186; -.
DR EnsemblPlants; AT5G62020.1; AT5G62020.1; AT5G62020.
DR GeneID; 836322; -.
DR Gramene; AT5G62020.1; AT5G62020.1; AT5G62020.
DR KEGG; ath:AT5G62020; -.
DR Araport; AT5G62020; -.
DR TAIR; locus:2174043; AT5G62020.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_3_2_1; -.
DR InParanoid; Q9SCW4; -.
DR OMA; STSHDAM; -.
DR OrthoDB; 1154048at2759; -.
DR PhylomeDB; Q9SCW4; -.
DR PRO; PR:Q9SCW4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SCW4; baseline and differential.
DR Genevisible; Q9SCW4; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..299
FT /note="Heat stress transcription factor B-2a"
FT /id="PRO_0000124587"
FT DNA_BIND 21..115
FT /evidence="ECO:0000250"
FT REGION 119..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..211
FT /note="Hydrophobic repeat HR-A/B"
FT MOTIF 261..264
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 34051 MW; 04D86398693B1997 CRC64;
MNSPPVDAMI TGESSSQRSI PTPFLTKTFN LVEDSSIDDV ISWNEDGSSF IVWNPTDFAK
DLLPKHFKHN NFSSFVRQLN TYGFKKVVPD RWEFSNDFFK RGEKRLLREI QRRKITTTHQ
TVVAPSSEQR NQTMVVSPSN SGEDNNNNQV MSSSPSSWYC HQTKTTGNGG LSVELLEENE
KLRSQNIQLN RELTQMKSIC DNIYSLMSNY VGSQPTDRSY SPGGSSSQPM EFLPAKRFSE
MEIEEEEEAS PRLFGVPIGL KRTRSEGVQV KTTAVVGENS DEETPWLRHY NRTNQRVCN
