HFLD_ECOLI
ID HFLD_ECOLI Reviewed; 213 AA.
AC P25746;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=High frequency lysogenization protein HflD;
GN Name=hflD; Synonyms=ycfC; OrderedLocusNames=b1132, JW5165;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8969519; DOI=10.1099/13500872-142-11-3219;
RA Green S.M., Malik T., Giles I.G., Drabble W.T.;
RT "The purB gene of Escherichia coli K-12 is located in an operon.";
RL Microbiology 142:3219-3230(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1729205; DOI=10.1128/jb.174.1.130-136.1992;
RA He B., Smith J.M., Zalkin H.;
RT "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation
RT by purR.";
RL J. Bacteriol. 174:130-136(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, SUBUNIT, INTERACTION WITH CII, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=11278968; DOI=10.1074/jbc.m011699200;
RA Kihara A., Akiyama Y., Ito K.;
RT "Revisiting the lysogenization control of bacteriophage lambda.
RT Identification and characterization of a new host component, HflD.";
RL J. Biol. Chem. 276:13695-13700(2001).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-213.
RG Midwest center for structural genomics (MCSG);
RT "Structure of a hypothetical protein ycfC coded by Escherichia coli
RT genome.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Negative regulator of phage lambda lysogenization.
CC Contributes to the degradation of the phage regulatory protein CII.
CC Acts probably by holding CII on the membrane surface, away from the
CC target promoters, but close to the FtsH protease.
CC {ECO:0000269|PubMed:11278968}.
CC -!- SUBUNIT: Interacts with CII protein from phage lambda.
CC {ECO:0000269|PubMed:11278968}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11278968}. Cell
CC inner membrane {ECO:0000269|PubMed:11278968}; Peripheral membrane
CC protein {ECO:0000269|PubMed:11278968}; Cytoplasmic side
CC {ECO:0000269|PubMed:11278968}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene show an increased
CC lysogenization frequency. {ECO:0000269|PubMed:11278968}.
CC -!- SIMILARITY: Belongs to the HflD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=M74924; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X59307; CAA41995.1; -; Genomic_DNA.
DR EMBL; M74924; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC74216.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35954.1; -; Genomic_DNA.
DR PIR; S19211; S19211.
DR RefSeq; NP_415650.1; NC_000913.3.
DR RefSeq; WP_001297479.1; NZ_STEB01000016.1.
DR PDB; 1QZ4; X-ray; 2.00 A; A=2-213.
DR PDB; 1SDI; X-ray; 1.65 A; A=2-213.
DR PDBsum; 1QZ4; -.
DR PDBsum; 1SDI; -.
DR AlphaFoldDB; P25746; -.
DR SMR; P25746; -.
DR BioGRID; 4263130; 20.
DR STRING; 511145.b1132; -.
DR jPOST; P25746; -.
DR PaxDb; P25746; -.
DR PRIDE; P25746; -.
DR EnsemblBacteria; AAC74216; AAC74216; b1132.
DR EnsemblBacteria; BAA35954; BAA35954; BAA35954.
DR GeneID; 67414325; -.
DR GeneID; 945693; -.
DR KEGG; ecj:JW5165; -.
DR KEGG; eco:b1132; -.
DR PATRIC; fig|1411691.4.peg.1134; -.
DR EchoBASE; EB1321; -.
DR eggNOG; COG2915; Bacteria.
DR HOGENOM; CLU_098920_0_0_6; -.
DR InParanoid; P25746; -.
DR OMA; RYIISLM; -.
DR PhylomeDB; P25746; -.
DR BioCyc; EcoCyc:EG11345-MON; -.
DR EvolutionaryTrace; P25746; -.
DR PRO; PR:P25746; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009408; P:response to heat; IEP:EcoliWiki.
DR Gene3D; 1.10.3890.10; -; 1.
DR HAMAP; MF_00695; HflD_protein; 1.
DR InterPro; IPR007451; HflD.
DR InterPro; IPR035932; HflD-like_sf.
DR PANTHER; PTHR38100; PTHR38100; 1.
DR Pfam; PF04356; DUF489; 1.
DR SUPFAM; SSF101322; SSF101322; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Coiled coil; Cytoplasm;
KW Membrane; Reference proteome.
FT CHAIN 1..213
FT /note="High frequency lysogenization protein HflD"
FT /id="PRO_0000071576"
FT COILED 79..126
FT /evidence="ECO:0000255"
FT HELIX 5..28
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 33..44
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 51..54
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 63..74
FT /evidence="ECO:0007829|PDB:1SDI"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 81..101
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 105..114
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 117..120
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 128..141
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 155..158
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 161..183
FT /evidence="ECO:0007829|PDB:1SDI"
FT HELIX 188..208
FT /evidence="ECO:0007829|PDB:1SDI"
SQ SEQUENCE 213 AA; 22948 MW; E26EF9698C3CEE42 CRC64;
MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS TLAVFGGSEA
NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS SAKGALDTLG NRINGLQRQL
EHFDLQSETL MSAMAAIYVD VISPLGPRIQ VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL
WHQVGGGRLQ LMFSRNRLTT QAKQILAHLT PEL