HFM1_MOUSE
ID HFM1_MOUSE Reviewed; 1434 AA.
AC D3Z4R1;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Probable ATP-dependent DNA helicase HFM1;
DE EC=3.6.4.12;
GN Name=Hfm1 {ECO:0000312|MGI:MGI:3036246};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=23555294; DOI=10.1371/journal.pgen.1003383;
RA Guiraldelli M.F., Eyster C., Wilkerson J.L., Dresser M.E., Pezza R.J.;
RT "Mouse HFM1/Mer3 is required for crossover formation and complete synapsis
RT of homologous chromosomes during meiosis.";
RL PLoS Genet. 9:E1003383-E1003383(2013).
CC -!- FUNCTION: Required for crossover formation and complete synapsis of
CC homologous chromosomes during meiosis. {ECO:0000269|PubMed:23555294}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- DISRUPTION PHENOTYPE: Male and female mutants are sterile due to severe
CC blockage of spermatogenesis and oogenesis.
CC {ECO:0000269|PubMed:23555294}.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000305}.
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DR EMBL; AC137947; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51582.1; -.
DR RefSeq; NP_808541.2; NM_177873.3.
DR AlphaFoldDB; D3Z4R1; -.
DR SMR; D3Z4R1; -.
DR BioGRID; 236907; 2.
DR STRING; 10090.ENSMUSP00000108310; -.
DR iPTMnet; D3Z4R1; -.
DR PhosphoSitePlus; D3Z4R1; -.
DR MaxQB; D3Z4R1; -.
DR PaxDb; D3Z4R1; -.
DR PRIDE; D3Z4R1; -.
DR ProteomicsDB; 269828; -.
DR Antibodypedia; 51797; 118 antibodies from 19 providers.
DR DNASU; 330149; -.
DR Ensembl; ENSMUST00000112690; ENSMUSP00000108310; ENSMUSG00000043410.
DR Ensembl; ENSMUST00000117588; ENSMUSP00000112590; ENSMUSG00000043410.
DR GeneID; 330149; -.
DR KEGG; mmu:330149; -.
DR UCSC; uc012eai.1; mouse.
DR CTD; 164045; -.
DR MGI; MGI:3036246; Hfm1.
DR VEuPathDB; HostDB:ENSMUSG00000043410; -.
DR eggNOG; KOG0952; Eukaryota.
DR GeneTree; ENSGT00550000074822; -.
DR HOGENOM; CLU_000335_0_0_1; -.
DR InParanoid; D3Z4R1; -.
DR OMA; CLKMDER; -.
DR OrthoDB; 154891at2759; -.
DR PhylomeDB; D3Z4R1; -.
DR TreeFam; TF328936; -.
DR BioGRID-ORCS; 330149; 1 hit in 72 CRISPR screens.
DR PRO; PR:D3Z4R1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; D3Z4R1; protein.
DR Bgee; ENSMUSG00000043410; Expressed in spermatocyte and 37 other tissues.
DR ExpressionAtlas; D3Z4R1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF02889; Sec63; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00973; Sec63; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Differentiation; Helicase; Hydrolase; Meiosis;
KW Nucleotide-binding; Oogenesis; Reference proteome; Spermatogenesis.
FT CHAIN 1..1434
FT /note="Probable ATP-dependent DNA helicase HFM1"
FT /id="PRO_0000430642"
FT DOMAIN 289..476
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 514..718
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 775..1089
FT /note="SEC63"
FT /evidence="ECO:0000255"
FT REGION 1110..1130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1294..1333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 410..413
FT /note="DEAH box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT COMPBIAS 1114..1130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1304..1329
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 302..309
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1434 AA; 161379 MW; 0ADD3ADB0DDA3731 CRC64;
MPKSDDCFFS MDNLFFSSPD ETENFPVKEK SLDWFLPPAP LISEIPDIQE LEEEIESYKL
LGKGKMPRML TSNLKIINED TNCISPTQKI HFSYNVHEQD YLNLGGSNNN DMSHVAGKLM
YGSSQKYKNH MGAKSPSARS SPGDTKLHDV AEDRQGTSAF KKRLSRTCDS EHDCDYADGS
LNLSSHISPV KLTQTKISKE NAWTCSNSKQ KRQYSTNKFK ANDAFSASGI GKDIFKAPSF
PAASQPHDIQ GITPNGLGSL KAVTEIPAKF RNIFKEFPYF NYIQSKAFDD LLYTDRNFVI
CAPTGSGKTV VFELAITRLL MEVPLPWLNM KIVYMAPIKA LCSQRFDDWK EKFGPVGLNC
KELTGDTVMD DLFEIQHANI IITTPEKWDS VTRKWRDNSF IQLVRLFLID EVHVIKDENR
GPTLEVVVSR MKTVQSLSRD LESASPVPVR FVAVSATIPN AEDIAEWLSD GERPAVCLKM
DESHRPVKLQ KVVLGFPCSS SQTEFKFDLA LNYKVYSVIR TYSDQKPTLV FCSTRKGVQQ
AASVLVKDAK FIISVEQKLR LQKSAYSIRD SKLKDTLVYG VGYHHAGMEL SDRKLVEGLF
TSGDLPVLFT TSTLAMGMNM PAHLVVIKST MHYSGGVFEE YSETDILQMI GRAGRPQFDT
TATAVIMTRL STREKYVQML ACNDTVESSL HRHLIEHLNA EIVLHTITDV NIALDWIRST
MLYIRALKNP SHYGFSSGLN KDGIEAKLQE LCLKNLKDLS SLDLIKMDED VNFKPTEAGR
LMAWYYITFE TVKKFCAISG KETLLDLISM ISSCNEFLDV QLRISEKRIL NTLNKDPNRI
TIRFPMAERI KTREMKVNCL IQAQLGCIPI QDFALTQDTV KIFRNGSRIA RWLSDFVAAQ
EKKFAVLLNS VILTKCFKCK LWENSKHVSK QLDKIGISLS NTMVNAGLTS FKKIEEANAR
ELELILNRHP PFGTQIKEAV AHLPKYELEV EQIARYSDIK AEILVTIILR NFEQLQTKRT
APDFHYATLI IGDADNQVVF KHKIMDSVLL KSGNWVKKID VKRALISEDL SINLISSDYV
GLDIHQKFTV FYFGPRKFVN ETAMERSSET DISHSDYSGR ATATGSSKGM ATCKKPGNRE
CHHHCKNKHA CGHDCCKIGV AQKPEVKESA MSSYLSDLKS RDAVSSLPLA KRLKIQMNKS
QNVDLKEFGF TPRPSLSSIS RSEYLNTPEL SILEQRNQHE IYGKVQQGPS EYRDKEVLGV
NLELGNEVWD DFDDESLIEV MSLSADAEKM AASGFGDTRD SSLGGSKLPF QKSSSRFQRD
NSNSFASSPG KPDAYLRDCS RSSFGLSSVA EIPQRAENAS LANLQERRPL TLSPVIERMC
FAHSKKTPQS PKFKEVDIFI GNSGSKKEID LSKYYPDDAA EEMKALLGIF NGIF
