ID   HFM1_XENTR              Reviewed;        1336 AA.
AC   A2RUV5; Q0V9K6;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Probable ATP-dependent DNA helicase HFM1;
DE            EC=3.6.4.12;
GN   Name=hfm1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for crossover formation and complete synapsis of
CC       homologous chromosomes during meiosis. {ECO:0000250|UniProtKB:D3Z4R1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI21499.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC133060; AAI33061.1; -; mRNA.
DR   EMBL; BC121498; AAI21499.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001072383.3; NM_001078915.2.
DR   AlphaFoldDB; A2RUV5; -.
DR   SMR; A2RUV5; -.
DR   STRING; 8364.ENSXETP00000062751; -.
DR   PRIDE; A2RUV5; -.
DR   DNASU; 779837; -.
DR   GeneID; 779837; -.
DR   KEGG; xtr:779837; -.
DR   CTD; 164045; -.
DR   Xenbase; XB-GENE-5894880; hfm1.
DR   eggNOG; KOG0952; Eukaryota.
DR   InParanoid; A2RUV5; -.
DR   OrthoDB; 154891at2759; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR004179; Sec63-dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF02889; Sec63; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00973; Sec63; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Meiosis; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..1336
FT                   /note="Probable ATP-dependent DNA helicase HFM1"
FT                   /id="PRO_0000324394"
FT   DOMAIN          159..347
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          388..589
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   DOMAIN          646..961
FT                   /note="SEC63"
FT   MOTIF           280..283
FT                   /note="DEAH box"
FT   BINDING         172..179
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   1336 AA;  151509 MW;  CFA610E5F6C8F1CB CRC64;
     MTLPFSWITN DVSQCIDLTQ DEHICSLSPV TDTEENMSAF KKSLFGKYDC SLNSEDQCLI
     VENGSSNFVT SKEAQKEAMK ADSDICPPQQ MAFNLFNETV VQKDMLNKEK FSHSASSNIS
     NKDDAQYGIV TPVTEIPKQF RTVFKEFPYF NYIQSKALEH LLYSDRNFVL CAPTGSGKTV
     IFELAIIRLL MQVPMPWTNV KIVYMAPIKA LCGQRYDDWK AKFGPVGLNC KELTGDTEMD
     DLFEIQHAHI IMTTPEKWDS MTRKWKDNTL VQLVRLFLID EVHILKEENR GATLEVVVSR
     MKTIYSLSHL SEDRKAFIPM RFVAVSATIP NVEDIADWLS DENSPGVCMK MDESSRPVKL
     RKVVLGFPCS TKQSEFKFDL TLNYKIANII QTYSDGRPTL VFCSTRKGVQ QAASILTKDA
     KFVMSIEHKQ RLQKCANSIK DSKLRDVLQY GVGYHHAGVD ISDRKVIENS FLIGDLPVLF
     TTSTLAMGVN LPAHLVIVKS TMHYVSGMFQ EYSETDILQM IGRAGRPQFD STATAVIMTR
     LSTKEKYVHM LDGADTIESS LHKHLVEHLN AEIALHTITD VKVALEWIRS TFLYIRALKN
     PAYYGFSEGL DKIGIEAKLQ ELCLKNLNDL SSLGLIKMDE EINFKPTETG KLMALYYIAF
     NTAKLFHTIR GTETLAELVS LISSCSEFSD VQLRANERRV LNTLNKDKNR VTIRYPMEGK
     IKTKEMKVNC LIQAHLGCIL VQDFSLTQDI SKIFRHGTRL AKCLSEFLAL QENKFSAFLN
     ALILTKCFKS KLWENSSHIS KQLEKIGVTL ANAMVNAGLT TFKKIEDTNA RELELIVNRH
     PPFGNQIKES VAHLPKYEIK FQQLAKYRAT TAEVVLTVLL TNFKQLQKKR TAPDSHFVML
     VIGDDDNQAI FKQKIMDSSL FKTGSWTRKI EIKRASKSTN VCLNLISSEY VGLDIQQSFN
     ICYLGSDSFN ADLVTQHKSE NIFSQEIYKT STKTKDIADT NWSMRQTAIK CATRECNHNC
     KNKDACGHEC CKVGVSEKAE MKTNFYSYLN DLRTRNSVSS ATPVKRLKMQ MEAETRNVNL
     QNFVFTPKPS IRSPCLKIPE NILLQAESSV LTNKYKASHQ EETCCKYEIK GCGEKQTKGM
     PKKTCERRNL TDSDKCNSSS SENAAYVTFD LGNDIWDDFD DDNLLDASNI SSLTDQHTSE
     FTDVSFSKNQ PVSKSSLSAG HHEEARYNAM SINKDSYRYL NAKSEVFSQE NTQCIDVVPS
     NQKLHGKMFS ENTIKHMFTL QEETNYPVTS KMDSPHNGCW SSNIFFKTPE NKTQERGCYH
     QEDETNAFIG IFNGIF