ID   HFQ_BRUA2               Reviewed;          78 AA.
AC   Q2YPW9; P0A3G9; Q57D26; Q9XBW1;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=RNA-binding protein Hfq {ECO:0000255|HAMAP-Rule:MF_00436};
GN   Name=hfq {ECO:0000255|HAMAP-Rule:MF_00436}; OrderedLocusNames=BAB1_1134;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN STRESS RESISTANCE
RP   PATHOGENESIS, AND DISRUPTION PHENOTYPE.
RX   PubMed=10564509; DOI=10.1046/j.1365-2958.1999.01629.x;
RA   Robertson G.T., Roop R.M. Jr.;
RT   "The Brucella abortus host factor I (HF-I) protein contributes to stress
RT   resistance during stationary phase and is a major determinant of virulence
RT   in mice.";
RL   Mol. Microbiol. 34:690-700(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
CC   -!- FUNCTION: RNA chaperone that binds small regulatory RNA (sRNAs) and
CC       mRNAs to facilitate mRNA translational regulation in response to
CC       envelope stress, environmental stress and changes in metabolite
CC       concentrations. Also binds with high specificity to tRNAs (By
CC       similarity). Contributes to long-term survival under nutrient
CC       deprivation, to stationary phase-dependent hydrogen peroxide and acid
CC       stress resistance and is required for prolonged survival in the
CC       intracellular environment of the host macrophage. Therefore,
CC       contributes not only to a generalized stress response in stationary
CC       phase but also to pathogenesis in mice, allowing the brucellae to adapt
CC       to the harsh conditions encountered within macrophages.
CC       {ECO:0000255|HAMAP-Rule:MF_00436, ECO:0000269|PubMed:10564509}.
CC   -!- DISRUPTION PHENOTYPE: Cells show increased sensitivity to hydrogen
CC       peroxide and decreased survival at pH 4.0 during stationary phase
CC       growth. It is less able to withstand prolonged starvation. It fails to
CC       replicate in cultured murine macrophages and is completely cleared from
CC       spleens and livers of infected mice 10 weeks after infection.
CC       {ECO:0000269|PubMed:10564509}.
CC   -!- SIMILARITY: Belongs to the Hfq family. {ECO:0000255|HAMAP-
CC       Rule:MF_00436}.
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DR   EMBL; AF154075; AAD38187.1; -; Genomic_DNA.
DR   EMBL; AM040264; CAJ11090.1; -; Genomic_DNA.
DR   RefSeq; WP_002964239.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YPW9; -.
DR   SMR; Q2YPW9; -.
DR   STRING; 359391.BAB1_1134; -.
DR   EnsemblBacteria; CAJ11090; CAJ11090; BAB1_1134.
DR   GeneID; 61317340; -.
DR   KEGG; bmf:BAB1_1134; -.
DR   PATRIC; fig|359391.11.peg.34; -.
DR   HOGENOM; CLU_113688_0_0_5; -.
DR   OMA; QQMVYKH; -.
DR   PRO; PR:Q2YPW9; -.
DR   Proteomes; UP000002719; Chromosome I.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd01716; Hfq; 1.
DR   HAMAP; MF_00436; Hfq; 1.
DR   InterPro; IPR005001; Hfq.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   PANTHER; PTHR34772; PTHR34772; 1.
DR   Pfam; PF17209; Hfq; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   TIGRFAMs; TIGR02383; Hfq; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; RNA-binding; Stress response; Virulence.
FT   CHAIN           1..78
FT                   /note="RNA-binding protein Hfq"
FT                   /id="PRO_0000095628"
SQ   SEQUENCE   78 AA;  8854 MW;  0D27DBD9CA161BD0 CRC64;
     MAERSQNLQD LFLNSVRKQK ISLTIFLING VKLTGIVTSF DNFCVLLRRD GHSQLVYKHA
     ISTIMPSQPV QMFEGEEA