3SIM4_DENAN
ID 3SIM4_DENAN Reviewed; 66 AA.
AC Q9PSN1;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Muscarinic toxin 4;
DE Short=MT4;
OS Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX NCBI_TaxID=8618;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=8585087; DOI=10.1016/0041-0101(95)00057-s;
RA Vandermeers A., Vandermeers-Piret M.-C., Rathe J., Waelbroeck M.,
RA Jolkkonen M., Oras A., Karlsson E.;
RT "Purification and sequence determination of a new muscarinic toxin (MT4)
RT from the venom of the green mamba (Dendroaspis angusticeps).";
RL Toxicon 33:1171-1179(1995).
CC -!- FUNCTION: Binds to the muscarinic acetylcholine receptor (CHRM).
CC {ECO:0000269|PubMed:8585087}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8585087}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=7489; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:8585087};
CC -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC residue stands at position 33 (Pro-31 in standard classification).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Aminergic toxin sub-subfamily. {ECO:0000305}.
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DR AlphaFoldDB; Q9PSN1; -.
DR SMR; Q9PSN1; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR InterPro; IPR035076; Toxin/TOLIP.
DR Pfam; PF00087; Toxin_TOLIP; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond;
KW G-protein coupled acetylcholine receptor impairing toxin;
KW G-protein coupled receptor impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin.
FT CHAIN 1..66
FT /note="Muscarinic toxin 4"
FT /evidence="ECO:0000269|PubMed:8585087"
FT /id="PRO_0000093643"
FT DISULFID 3..24
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 17..42
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 46..58
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 59..64
FT /evidence="ECO:0000250|UniProtKB:P60301"
SQ SEQUENCE 66 AA; 7499 MW; EAB0F54F4A00DD3E CRC64;
LTCVTSKSIF GITTENCPDG QNLCFKKWYY IVPRYSDITW GCAATCPKPT NVRETIHCCE
TDKCNE