ID   3SIM6_OPHHA             Reviewed;          86 AA.
AC   Q2VBN1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Muscarinic toxin MTX6;
DE   Flags: Precursor;
OS   Ophiophagus hannah (King cobra) (Naja hannah).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Ophiophagus.
OX   NCBI_TaxID=8665;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=16689684; DOI=10.1042/bj20060004;
RA   Li J., Zhang H., Liu J., Xu K.;
RT   "Novel genes encoding six kinds of three-finger toxins in Ophiophagus
RT   hannah (king cobra) and function characterization of two recombinant long-
RT   chain neurotoxins.";
RL   Biochem. J. 398:233-242(2006).
CC   -!- FUNCTION: Binds to the muscarinic acetylcholine receptor (CHRM).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC       residue stands at position 54 (Pro-31 in standard classification).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Aminergic toxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; DQ273584; ABB83638.1; -; mRNA.
DR   AlphaFoldDB; Q2VBN1; -.
DR   SMR; Q2VBN1; -.
DR   TopDownProteomics; Q2VBN1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   InterPro; IPR035076; Toxin/TOLIP.
DR   Pfam; PF00087; Toxin_TOLIP; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; G-protein coupled acetylcholine receptor impairing toxin;
KW   G-protein coupled receptor impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000250"
FT   CHAIN           22..86
FT                   /note="Muscarinic toxin MTX6"
FT                   /id="PRO_5000006494"
FT   DISULFID        24..45
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        38..63
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        67..78
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        79..84
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
SQ   SEQUENCE   86 AA;  9412 MW;  0C5191F52526A24F CRC64;
     MKTLLLTLVV VTILCLDLGY TLTCLTHESL FFETTETCSD GQNLCYAKWF AVFPGARRPD
     GGCAATCPDK VPLEIVNCCT TDKCNL