HFW1_DROME
ID HFW1_DROME Reviewed; 611 AA.
AC Q9W568; O46061; Q8SZE5;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 3.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Protein halfway;
DE AltName: Full=Protein singed wings;
DE Flags: Precursor;
GN Name=hfw {ECO:0000312|FlyBase:FBgn0001189};
GN Synonyms=swi {ECO:0000312|EMBL:CAF25166.1}; ORFNames=CG3095;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAF25166.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF CYS-379, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=y swi w {ECO:0000312|EMBL:CAF25166.1};
RX PubMed=15189568; DOI=10.1186/1471-2156-5-15;
RA Schwartz Y.B., Boykova T., Belyaeva E.S., Ashburner M., Zhimulev I.F.;
RT "Molecular characterization of the singed wings locus of Drosophila
RT melanogaster.";
RL BMC Genet. 5:15-15(2004).
RN [2] {ECO:0000312|EMBL:AAF45654.3}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF45654.3}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:CAA16810.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000269|PubMed:10731137};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5] {ECO:0000312|EMBL:AAL48553.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48553.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-221 AND ASN-246, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous system
RT of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
RN [7]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246; ASN-264 AND ASN-269, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
CC -!- FUNCTION: Has a role in the ecdysone induced cascade; probably indirect
CC control of 'late' ecdysone genes. {ECO:0000269|PubMed:15189568}.
CC -!- DISRUPTION PHENOTYPE: Mutants specifically interrupt the transmission
CC of the regulatory signal from early to late ecdysone inducible genes.
CC {ECO:0000269|PubMed:15189568}.
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DR EMBL; AJ626646; CAF25166.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45654.3; -; Genomic_DNA.
DR EMBL; AL021726; CAA16810.1; -; Genomic_DNA.
DR EMBL; AY070931; AAL48553.1; -; mRNA.
DR RefSeq; NP_001259135.1; NM_001272206.1.
DR RefSeq; NP_569933.2; NM_130577.4.
DR AlphaFoldDB; Q9W568; -.
DR SMR; Q9W568; -.
DR BioGRID; 57671; 1.
DR STRING; 7227.FBpp0305326; -.
DR GlyGen; Q9W568; 4 sites.
DR iPTMnet; Q9W568; -.
DR PaxDb; Q9W568; -.
DR PRIDE; Q9W568; -.
DR DNASU; 31120; -.
DR EnsemblMetazoa; FBtr0070270; FBpp0070260; FBgn0001189.
DR EnsemblMetazoa; FBtr0333115; FBpp0305326; FBgn0001189.
DR GeneID; 31120; -.
DR KEGG; dme:Dmel_CG3095; -.
DR CTD; 31120; -.
DR FlyBase; FBgn0001189; hfw.
DR VEuPathDB; VectorBase:FBgn0001189; -.
DR eggNOG; KOG0619; Eukaryota.
DR HOGENOM; CLU_028059_2_0_1; -.
DR InParanoid; Q9W568; -.
DR OMA; RLQCDCN; -.
DR OrthoDB; 500288at2759; -.
DR PhylomeDB; Q9W568; -.
DR Reactome; R-DME-114608; Platelet degranulation.
DR BioGRID-ORCS; 31120; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Iswi; fly.
DR GenomeRNAi; 31120; -.
DR PRO; PR:Q9W568; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0001189; Expressed in spermathecum and 34 other tissues.
DR ExpressionAtlas; Q9W568; baseline and differential.
DR Genevisible; Q9W568; DM.
DR GO; GO:0035556; P:intracellular signal transduction; IMP:UniProtKB.
DR GO; GO:0035073; P:pupariation; IMP:UniProtKB.
DR GO; GO:0035075; P:response to ecdysone; IMP:UniProtKB.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13855; LRR_8; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Leucine-rich repeat; Reference proteome; Repeat; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..611
FT /note="Protein halfway"
FT /id="PRO_0000021413"
FT REPEAT 236..257
FT /note="LRR 1"
FT REPEAT 259..280
FT /note="LRR 2"
FT REPEAT 283..304
FT /note="LRR 3"
FT REPEAT 313..334
FT /note="LRR 4"
FT DOMAIN 361..416
FT /note="LRRNT"
FT REPEAT 417..438
FT /note="LRR 5"
FT REPEAT 443..464
FT /note="LRR 6"
FT REPEAT 468..489
FT /note="LRR 7"
FT DOMAIN 505..554
FT /note="LRRCT"
FT REGION 31..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 221
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
FT CARBOHYD 246
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096,
FT ECO:0000269|PubMed:19349973"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 269
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT MUTAGEN 379
FT /note="C->Y: In swi-t467; lethal during puparium
FT formation."
FT /evidence="ECO:0000269|PubMed:15189568"
FT CONFLICT 84
FT /note="E -> K (in Ref. 5; AAL48553)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 611 AA; 68545 MW; E8FE83F1F00DFB13 CRC64;
MLAYTHGTWL LLLLLLVAGA CARPEIAHTP DPAALGEEST PHAHAHPQAR HHHHAHPHAP
LKEDEQGTAI PAPILTTDNV GIGETTTASS LAETQSMSDP GSVTDTTSTS TSHSTSTTST
TSPAPLPPAA PEQPEYLKHC FYAEEQLCGH TFDGRAETSE GQGSTVAQSE AQNRGGQGNS
QCQCREHPTR PNSWYCCNIS QLTMISSCSN ISKWTNLHVR NMTVEDMDLS NPIFRSLQSL
AVTDGNITRL VNAFPRLSAL KCLNISNNNI SEIHSRAVKD VPHLEFFGMS NNNLSLVPHR
NQNKNITLDI SGNMRMLCTP LNEIIYTESI NFLNPKHSYC QYNATHTWFQ STDKVSVEQL
ENRKRCVTNC PVIPNYGSCN CTLENIMIIQ DNQSKPQCHV DCSNLGLVEL PQRLPDNTFM
LNITNNKITS LGDYFHTNPT YHNINRLLAD NNQISSIYEF EGTKFIETFQ RIYMRNNSLS
KIPEYFLNNA LMDSGLGRRI YLAGNKLQCD CNSAKTLQNW LKERSSDIPD YMEIRCRNMP
QRVIELQEAK LCQSPPDWTD YIYYLIAAEV LLLLALITKV SYDYWVFKTA GYLPWPASKM
PKLPCDWLCE S
