HGBA_HAEDU
ID HGBA_HAEDU Reviewed; 972 AA.
AC Q47952; Q47957;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 09-SEP-2003, sequence version 2.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Hemoglobin and hemoglobin-haptoglobin-binding protein {ECO:0000305};
DE Flags: Precursor;
GN Name=hgbA {ECO:0000303|PubMed:7890379};
GN Synonyms=hupA {ECO:0000303|PubMed:8613384};
GN OrderedLocusNames=HD_2025 {ECO:0000312|EMBL:AAP96732.1};
OS Haemophilus ducreyi (strain 35000HP / ATCC 700724).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=233412;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=35000HP / ATCC 700724;
RX PubMed=7768598; DOI=10.1128/iai.63.6.2194-2200.1995;
RA Elkins C., Chen C.J., Thomas C.E.;
RT "Characterization of the hgbA locus encoding a hemoglobin receptor from
RT Haemophilus ducreyi.";
RL Infect. Immun. 63:2194-2200(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=35000HP / ATCC 700724;
RX PubMed=8613384; DOI=10.1128/iai.64.5.1724-1735.1996;
RA Stevens M.K., Porcella S.F., Klesney-Tait J., Lumbley S., Thomas S.E.,
RA Norgard M.V., Radolf J.R., Hansen E.J.;
RT "A hemoglobin-binding outer membrane protein is involved in virulence
RT expression by Haemophilus ducreyi in an animal model.";
RL Infect. Immun. 64:1724-1735(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=35000HP / ATCC 700724;
RA Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L.,
RA Nguyen D., Wang J., Forst C., Hood L.;
RT "The complete genome sequence of Haemophilus ducreyi.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PROTEIN SEQUENCE OF 23-36, FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=35000HP / ATCC 700724;
RX PubMed=7890379; DOI=10.1128/iai.63.4.1241-1245.1995;
RA Elkins C.;
RT "Identification and purification of a conserved heme-regulated hemoglobin-
RT binding outer membrane protein from Haemophilus ducreyi.";
RL Infect. Immun. 63:1241-1245(1995).
CC -!- FUNCTION: Acts as a receptor for hemoglobin or the
CC hemoglobin/haptoglobin complex of the host and is required for heme
CC uptake. May be involved in virulence. {ECO:0000269|PubMed:7890379,
CC ECO:0000269|PubMed:8613384}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:7890379,
CC ECO:0000269|PubMed:8613384}.
CC -!- DISRUPTION PHENOTYPE: Mutant cannot utilize hemoglobin as the sole
CC source of heme for growth. Mutation attenuates the virulence.
CC {ECO:0000269|PubMed:8613384}.
CC -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC Hemoglobin/haptoglobin binding protein subfamily. {ECO:0000305}.
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DR EMBL; U17281; AAA75311.1; -; Genomic_DNA.
DR EMBL; U34048; AAC44054.1; -; Genomic_DNA.
DR EMBL; AE017143; AAP96732.1; -; Genomic_DNA.
DR RefSeq; WP_010945753.1; NC_002940.2.
DR AlphaFoldDB; Q47952; -.
DR SMR; Q47952; -.
DR STRING; 233412.HD_2025; -.
DR EnsemblBacteria; AAP96732; AAP96732; HD_2025.
DR KEGG; hdu:HD_2025; -.
DR eggNOG; COG1629; Bacteria.
DR eggNOG; COG4771; Bacteria.
DR HOGENOM; CLU_008287_19_0_6; -.
DR OMA; VFSRLTY; -.
DR Proteomes; UP000001022; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 2.170.130.10; -; 1.
DR Gene3D; 2.40.170.20; -; 1.
DR InterPro; IPR039426; BtuB-like.
DR InterPro; IPR012910; Plug_dom.
DR InterPro; IPR037066; Plug_dom_sf.
DR InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR InterPro; IPR010949; TonB_Hb/transfer/lactofer_rcpt.
DR InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR PANTHER; PTHR30069; PTHR30069; 1.
DR Pfam; PF07715; Plug; 1.
DR Pfam; PF00593; TonB_dep_Rec; 1.
DR TIGRFAMs; TIGR01786; TonB-hemlactrns; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Direct protein sequencing; Membrane; Receptor;
KW Reference proteome; Signal; TonB box; Transmembrane;
KW Transmembrane beta strand; Transport; Virulence.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:7890379,
FT ECO:0000303|PubMed:8613384"
FT CHAIN 23..972
FT /note="Hemoglobin and hemoglobin-haptoglobin-binding
FT protein"
FT /id="PRO_0000034789"
FT MOTIF 32..39
FT /note="TonB box"
FT MOTIF 955..972
FT /note="TonB C-terminal box"
FT CONFLICT 55
FT /note="A -> S (in Ref. 1; AAA75311)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="G -> V (in Ref. 2; AAC44054)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 972 AA; 110921 MW; 535A45288A97A792 CRC64;
MKANKLSAIT LCILGYAHTV YAESNMQTEK LETIVVSSED DSVHNKNVGE IKKNAKALSK
QQVQDSRDLV RYETGVTVVE KGRFGSSGYA IRGVDENRVA VVVDGLHQAE TISSQGFKEL
FEGYGNFNNT RNGVEVENLK QAVIQKGADA IRTGSGSLGG TVSFESKDAR DYLIDKNYHF
GYKTGYSSAD NQKLHSVTAA GRYSDFDLLA VHTQRHGNEL RNYGYRHYDG SVVRKEREKA
DPYKITKQSS LIKIGYQLND TNRFTLGYDD SRNTSRGTDW SNAFTSYNGG PFLKDVRHTN
DQSNRKNISF VYENFDTNDF WDTLKITHNH QKIKLKARLD EYCDVNGEID CPAIANPSGL
YINDKGIFLD KHDGEITHKK EGEFNNYFDS KGKEVRVKGF NVDSILINCD QYDCSKPMQL
LSSTNNGYGG SPNKYIYKTY ELFEKTMNNG NGKYAVLEIR SSGHEKFSRV YLPSEKGYVE
NQWKDRDLNT DTQQYNIDLT KSFKLKSVEH NATYGGLYSE VKKSMTNRAG YEAYNRQWWA
NIFFGKENNK PNKCQPYNGN SFTTLCSHED RLFSFLIPVK TKTGALYVTD KIKLNDKVNL
DVAYRYDRIK HDPKYIPGTT PKLPTDLILG RFIEFKPKNT YATQDEKNEN AEKNAVYLAS
KKTKFSANSY SATFSFDPMD FLKIQAKYAT GFRAPTSDEI YFVFQHPSFS IYPNLYLKAE
RSKNKEVAIT LHKQKSFLTV NLFQTDYKDF LDLAYLKKGS LPYGNGGSQL ETLLYQNVNR
DKARVKGLEV NSKLHLGDVW RTLDGFNLSY KLSLQKGRMS SKVGEEGKQR DTNKLDTPMN
AIQPQTHVVG VGYEHPQEKF GVDMYLTHAS AKKEKDTFNM FYDGKDQKDQ HIKWRSDRYT
LVDLIAYVKP VKNVTLRAGV YNLTNREYGT WDSIRSIRPF GTTNLINQET GKGIKRFNAP
GRNFRVNAEI TF
