HGD_DICDI
ID HGD_DICDI Reviewed; 432 AA.
AC Q54QI4; Q9GU65;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Homogentisate 1,2-dioxygenase;
DE EC=1.13.11.5;
DE AltName: Full=Homogentisate oxygenase;
DE AltName: Full=Homogentisic acid oxidase;
DE AltName: Full=Homogentisicase;
GN Name=hgd; Synonyms=hgo; ORFNames=DDB_G0283765;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2-432.
RA Schmidt S.R.;
RT "Identification of HGO homologs in different organisms.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=homogentisate + O2 = 4-maleylacetoacetate + H(+);
CC Xref=Rhea:RHEA:15449, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16169, ChEBI:CHEBI:17105; EC=1.13.11.5;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC acetoacetate and fumarate from L-phenylalanine: step 4/6.
CC -!- SIMILARITY: Belongs to the homogentisate dioxygenase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000057; EAL65497.1; -; Genomic_DNA.
DR EMBL; AF189237; AAG17115.1; -; mRNA.
DR RefSeq; XP_638887.1; XM_633795.1.
DR AlphaFoldDB; Q54QI4; -.
DR SMR; Q54QI4; -.
DR BioGRID; 1249288; 1.
DR STRING; 44689.DDB0191461; -.
DR PaxDb; Q54QI4; -.
DR EnsemblProtists; EAL65497; EAL65497; DDB_G0283765.
DR GeneID; 8624285; -.
DR KEGG; ddi:DDB_G0283765; -.
DR dictyBase; DDB_G0283765; hgd.
DR eggNOG; KOG1417; Eukaryota.
DR HOGENOM; CLU_027174_0_0_1; -.
DR InParanoid; Q54QI4; -.
DR OMA; FMFETRW; -.
DR PhylomeDB; Q54QI4; -.
DR Reactome; R-DDI-8963684; Tyrosine catabolism.
DR UniPathway; UPA00139; UER00339.
DR PRO; PR:Q54QI4; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0004411; F:homogentisate 1,2-dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006559; P:L-phenylalanine catabolic process; IBA:GO_Central.
DR GO; GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR005708; Homogentis_dOase.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR11056; PTHR11056; 1.
DR Pfam; PF04209; HgmA; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR TIGRFAMs; TIGR01015; hmgA; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Phenylalanine catabolism;
KW Reference proteome; Tyrosine catabolism.
FT CHAIN 1..432
FT /note="Homogentisate 1,2-dioxygenase"
FT /id="PRO_0000312407"
FT BINDING 333
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 339
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 369
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT CONFLICT 2..4
FT /note="ALE -> TRP (in Ref. 2; AAG17115)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 432 AA; 48234 MW; F704C3DCA58CAC53 CRC64;
MALEQIDYEY QSGFGNSFES EAIKGTLPKG RNAPQNCPLD LYAEQLSGNA FTAPRHTQQR
SWLYRIRPSV CHTPLKPIDS GLVCDLNNLH VDPNQLRWKP FPITEDKPHD FVEGLITIAG
AGHASVRHGL AIHIYTATKS MENKSFYNSD GDFLIVPQQG TLDIQTEFGF MKVKSGEICV
IQRGITFSVN VEGPTRGYIC EVFGSHFKLP DLGPIGANGL ANPRDFLSPV AAYEKKEGIE
HTKINKFLGK LFSATQTYSP FNVVAWHGNY CPYKYDLSLF CVVNSVSFDH LDPSIFTVLT
APTNEVGVAA ADFVIFPPRW LVQENTFRPP YFHRNCMSEF MGLIRGVYEA KKEGFLPGGG
SLHSCMTPHG PDSDTFYAAI KAELKPTKIP DVALAFMFES SLILGISDYA KKNFIDDDYW
KCWQGLKDNS KI
