ID   HGD_ORYSJ               Reviewed;         470 AA.
AC   Q5VRH4; A0A0P0WRA6; Q0DFB9;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Homogentisate 1,2-dioxygenase;
DE            EC=1.13.11.5;
DE   AltName: Full=Homogentisate oxygenase;
DE   AltName: Full=Homogentisic acid oxidase;
DE   AltName: Full=Homogentisicase;
GN   Name=HGO;
GN   OrderedLocusNames=Os06g0103300 {ECO:0000312|EMBL:BAF18454.1},
GN   LOC_Os06g01360 {ECO:0000305};
GN   ORFNames=OsJ_19811 {ECO:0000312|EMBL:EEE64940.1}, OSJNBa0075G19.26;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=homogentisate + O2 = 4-maleylacetoacetate + H(+);
CC         Xref=Rhea:RHEA:15449, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16169, ChEBI:CHEBI:17105; EC=1.13.11.5;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC       acetoacetate and fumarate from L-phenylalanine: step 4/6.
CC   -!- SIMILARITY: Belongs to the homogentisate dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; AP002842; BAD67951.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF18454.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS95715.1; -; Genomic_DNA.
DR   EMBL; CM000143; EEE64940.1; -; Genomic_DNA.
DR   EMBL; AK065189; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015643393.1; XM_015787907.1.
DR   AlphaFoldDB; Q5VRH4; -.
DR   SMR; Q5VRH4; -.
DR   STRING; 4530.OS06T0103300-01; -.
DR   PaxDb; Q5VRH4; -.
DR   PRIDE; Q5VRH4; -.
DR   EnsemblPlants; Os06t0103300-01; Os06t0103300-01; Os06g0103300.
DR   GeneID; 4339844; -.
DR   Gramene; Os06t0103300-01; Os06t0103300-01; Os06g0103300.
DR   KEGG; osa:4339844; -.
DR   eggNOG; KOG1417; Eukaryota.
DR   HOGENOM; CLU_027174_0_0_1; -.
DR   InParanoid; Q5VRH4; -.
DR   OMA; FMFETRW; -.
DR   OrthoDB; 795654at2759; -.
DR   PlantReactome; R-OSA-1119506; tyrosine degradation I.
DR   UniPathway; UPA00139; UER00339.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q5VRH4; baseline and differential.
DR   Genevisible; Q5VRH4; OS.
DR   GO; GO:0004411; F:homogentisate 1,2-dioxygenase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IBA:GO_Central.
DR   GO; GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR005708; Homogentis_dOase.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR11056; PTHR11056; 1.
DR   Pfam; PF04209; HgmA; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   TIGRFAMs; TIGR01015; hmgA; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Iron; Metal-binding; Oxidoreductase; Phenylalanine catabolism;
KW   Reference proteome; Tyrosine catabolism.
FT   CHAIN           1..470
FT                   /note="Homogentisate 1,2-dioxygenase"
FT                   /id="PRO_0000247478"
FT   BINDING         356
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         362
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         392
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        421
FT                   /note="M -> V (in Ref. 5; AK065189)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   470 AA;  51771 MW;  9C2148F4ABB079E4 CRC64;
     MAMATATPAA QNEQQEKGGL EYVYLSGLGN SLSSEAVAGT LPRGQNSPLV CPLGLYAEQL
     SGTPFTAPRA RNLRTWLYRI KPSVTHEPFH PRRPAHPRLI GDFDRTTTDT VATPTQLRWR
     PADVPPHHPP LDFIDGLYTV CGAGSSFLRH GYAIHMYAAN KSMDGCAFCN ADGDFLIVPQ
     QGKLLITTEC GKLLVPPGEI VVIPQGFRFA VDLPDGPSRG YVSEIFGTHF QLPDLGPIGA
     NGLASARDFL SPTAWFEQVH RPGYTIVQKY GGELFTATQD FSPFNVVAWH GNYVPYKYDL
     SKFCPFNTVL FDHADPSVNT VLTAPTDKPG VALLDFVIFP PRWLVAENTF RPPYYHRNCM
     SEFMGLIYGI YEAKADGFLP GGASLHSCMT PHGPDTKTYE ATISRPDANE PSRLSGTLAF
     MFESALIPRV CQWALDSPSR DLDYYQCWIG LKSHFSHDNG GATSEEPCRK