HGGT_ORYSJ
ID HGGT_ORYSJ Reviewed; 404 AA.
AC Q0DAK7; Q67W53; Q7XB12;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 29-OCT-2014, sequence version 2.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Homogentisate geranylgeranyltransferase, chloroplastic {ECO:0000303|PubMed:12897790};
DE Short=OsHGGT {ECO:0000303|PubMed:21175184};
DE EC=2.5.1.116 {ECO:0000250|UniProtKB:Q7XB14};
DE Flags: Precursor;
GN Name=HGGT {ECO:0000303|PubMed:12897790};
GN OrderedLocusNames=Os06g0646900 {ECO:0000312|EMBL:BAF20116.2},
GN LOC_Os06g43880 {ECO:0000305};
GN ORFNames=OJ1226_A12.22 {ECO:0000312|EMBL:BAD37616.1},
GN OSJNBa0062J02.6 {ECO:0000312|EMBL:BAD37319.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|EMBL:BAF20116.2};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Taichung 65;
RX PubMed=12897790; DOI=10.1038/nbt853;
RA Cahoon E.B., Hall S.E., Ripp K.G., Ganzke T.S., Hitz W.D., Coughlan S.J.;
RT "Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol
RT production and increased antioxidant content.";
RL Nat. Biotechnol. 21:1082-1087(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION.
RX PubMed=21175184; DOI=10.1021/jf104012z;
RA Kim Y.H., Lee Y.Y., Kim Y.H., Choi M.S., Jeong K.H., Lee S.K., Seo M.J.,
RA Yun H.T., Lee C.K., Kim W.H., Lee S.C., Park S.K., Park H.M.;
RT "Antioxidant activity and inhibition of lipid peroxidation in germinating
RT seeds of transgenic soybean expressing OsHGGT.";
RL J. Agric. Food Chem. 59:584-591(2011).
CC -!- FUNCTION: Involved in the synthesis of tocotrienol (vitamin E)
CC (PubMed:21175184). Catalyzes the condensation of homogentisate and
CC geranylgeranyl diphosphate to form 2-methyl-6-
CC geranylgeranylbenzoquinol. Possesses low activity with phytyl
CC diphosphate as substrate (By similarity). Tocotrienols functions to
CC limit lipid peroxidation during seed germination (PubMed:21175184).
CC {ECO:0000250|UniProtKB:Q7XB14, ECO:0000269|PubMed:21175184}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E,10E)-geranylgeranyl diphosphate + H(+) + homogentisate
CC = 6-geranylgeranyl-2-methylbenzene-1,4-diol + CO2 + diphosphate;
CC Xref=Rhea:RHEA:38003, ChEBI:CHEBI:15378, ChEBI:CHEBI:16169,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:58756,
CC ChEBI:CHEBI:75411; EC=2.5.1.116;
CC Evidence={ECO:0000250|UniProtKB:Q7XB14};
CC -!- PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000250|UniProtKB:Q7XB14}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- MISCELLANEOUS: Seeds of most monocots are enriched in tocotrienols and
CC contain only small amounts of tocopherols.
CC {ECO:0000303|PubMed:12897790}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD37319.1; Type=Erroneous gene model prediction;
CC Sequence=BAD37616.1; Type=Erroneous gene model prediction;
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DR EMBL; AY222862; AAP43913.1; -; mRNA.
DR EMBL; AP003517; BAD37319.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP004008; BAD37616.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008212; BAF20116.2; -; Genomic_DNA.
DR EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015641999.1; XM_015786513.1.
DR AlphaFoldDB; Q0DAK7; -.
DR SMR; Q0DAK7; -.
DR STRING; 4530.OS06T0646900-01; -.
DR PaxDb; Q0DAK7; -.
DR PRIDE; Q0DAK7; -.
DR GeneID; 4341663; -.
DR KEGG; osa:4341663; -.
DR InParanoid; Q0DAK7; -.
DR OrthoDB; 1231215at2759; -.
DR BRENDA; 2.5.1.116; 8948.
DR UniPathway; UPA00160; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102551; F:homogentisate geranylgeranyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004659; F:prenyltransferase activity; IEA:InterPro.
DR GO; GO:0010189; P:vitamin E biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd13960; PT_UbiA_HPT1; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR InterPro; IPR044502; AtHST-like.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR044878; UbiA_sf.
DR Pfam; PF01040; UbiA; 1.
DR PROSITE; PS00189; LIPOYL; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transferase;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..81
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 82..404
FT /note="Homogentisate geranylgeranyltransferase,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000430736"
FT TRANSMEM 114..134
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
SQ SEQUENCE 404 AA; 44900 MW; CC79F6D2A5C7BDD3 CRC64;
MQASSAAAAA ACSAIKPAAH QHTVQVQEDK RGSEFRARFG TRKLSWGGKL SVENSALHQC
QSLTRSIRRQ KRQHSPVLQV RCYAIAGDQH ESIATEFEEI CKEVPQKLGA FYRFCRPHTI
FGTIIGITSV SLLPMRSLDD FTMKALWGFL EALSSSLCMN IYVVGLNQLY DIQIDKVNKP
SLPLASGEFS VATGAVLVLT SLIMSIAIGI RSKSAPLLCA LFISFFLGSA YSVDAPLLRW
KRNAFLAASC ILFVRAVLVQ LAFFAHMQQH VLKRPLAPTK SVVFATLFMC CFSSVIALFK
DIPDIDGDRH FGVESLSVRL GPERVYWLCI NILLTAYGAA ILAGASSTNL CQMIITVFGH
GLLAFALWQR AQHCDVENKA WITSFYMFIW KLFYAEYFLI PFVQ
