ID   HHA_ECO57               Reviewed;          72 AA.
AC   P0ACE5; P23870; P77120;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Hemolysin expression-modulating protein Hha;
GN   Name=hha; OrderedLocusNames=Z0573, ECs0513;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Down-regulates hemolysin expression, in complex with H-NS,
CC       and can also stimulate transposition events in vivo. Modifies the set
CC       of genes regulated by H-NS; Hha and Cnu (YdgT) increase the number of
CC       genes DNA bound by H-NS/StpA and may also modulate the oligomerization
CC       of the H-NS/StpA-complex. Binds DNA and influences DNA topology in
CC       response to environmental stimuli. Decreases biofilm formation (By
CC       similarity). {ECO:0000250|UniProtKB:P0ACE3}.
CC   -!- SUBUNIT: Forms a heterotrimeric complex with the H-NS dimer.
CC       {ECO:0000250|UniProtKB:P0ACE3}.
CC   -!- SIMILARITY: Belongs to the Hha/YmoA/Cnu family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG54809.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33936.1; -; Genomic_DNA.
DR   PIR; A99693; A99693.
DR   PIR; E85543; E85543.
DR   RefSeq; NP_308540.1; NC_002695.1.
DR   RefSeq; WP_001291435.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0ACE5; -.
DR   BMRB; P0ACE5; -.
DR   SMR; P0ACE5; -.
DR   STRING; 155864.EDL933_0535; -.
DR   EnsemblBacteria; AAG54809; AAG54809; Z0573.
DR   EnsemblBacteria; BAB33936; BAB33936; ECs_0513.
DR   GeneID; 66671238; -.
DR   GeneID; 914617; -.
DR   KEGG; ece:Z0573; -.
DR   KEGG; ecs:ECs_0513; -.
DR   PATRIC; fig|386585.9.peg.618; -.
DR   eggNOG; ENOG5032SGA; Bacteria.
DR   HOGENOM; CLU_190629_0_0_6; -.
DR   OMA; RRCQSID; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:1900191; P:negative regulation of single-species biofilm formation; IMP:CACAO.
DR   GO; GO:2000147; P:positive regulation of cell motility; IMP:CACAO.
DR   Gene3D; 1.20.1280.40; -; 1.
DR   InterPro; IPR007985; Hemolysn_expr_modulating_HHA.
DR   InterPro; IPR036666; HHA_sf.
DR   Pfam; PF05321; HHA; 1.
DR   SUPFAM; SSF68989; SSF68989; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..72
FT                   /note="Hemolysin expression-modulating protein Hha"
FT                   /id="PRO_0000201728"
FT   SITE            25
FT                   /note="Interacts with H-NS"
FT                   /evidence="ECO:0000250"
FT   SITE            48
FT                   /note="Interacts with H-NS"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   72 AA;  8628 MW;  A77211B87CF0134A CRC64;
     MSEKPLTKTD YLMRLRRCQT IDTLERVIEK NKYELSDNEL AVFYSAADHR LAELTMNKLY
     DKIPSSVWKF IR