ID   HHA_ECOL6               Reviewed;          72 AA.
AC   P0ACE4; P23870; P77120;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Hemolysin expression-modulating protein Hha;
GN   Name=hha; OrderedLocusNames=c0578;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Down-regulates hemolysin expression, in complex with H-NS,
CC       and can also stimulate transposition events in vivo. Modifies the set
CC       of genes regulated by H-NS; Hha and Cnu (YdgT) increase the number of
CC       genes DNA bound by H-NS/StpA and may also modulate the oligomerization
CC       of the H-NS/StpA-complex. Binds DNA and influences DNA topology in
CC       response to environmental stimuli. Decreases biofilm formation (By
CC       similarity). {ECO:0000250|UniProtKB:P0ACE3}.
CC   -!- SUBUNIT: Forms a heterotrimeric complex with the H-NS dimer.
CC       {ECO:0000250|UniProtKB:P0ACE3}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the tomB-hha operon causes reduced
CC       infection of the mouse bladder but not of the kidney.
CC       {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the Hha/YmoA/Cnu family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN79056.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN79056.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001291435.1; NC_004431.1.
DR   AlphaFoldDB; P0ACE4; -.
DR   BMRB; P0ACE4; -.
DR   SMR; P0ACE4; -.
DR   STRING; 199310.c0578; -.
DR   EnsemblBacteria; AAN79056; AAN79056; c0578.
DR   GeneID; 66671238; -.
DR   KEGG; ecc:c0578; -.
DR   eggNOG; ENOG5032SGA; Bacteria.
DR   HOGENOM; CLU_190629_0_0_6; -.
DR   OMA; RRCQSID; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1280.40; -; 1.
DR   InterPro; IPR007985; Hemolysn_expr_modulating_HHA.
DR   InterPro; IPR036666; HHA_sf.
DR   Pfam; PF05321; HHA; 1.
DR   SUPFAM; SSF68989; SSF68989; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Repressor; Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..72
FT                   /note="Hemolysin expression-modulating protein Hha"
FT                   /id="PRO_0000201729"
FT   SITE            25
FT                   /note="Interacts with H-NS"
FT                   /evidence="ECO:0000250"
FT   SITE            48
FT                   /note="Interacts with H-NS"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   72 AA;  8628 MW;  A77211B87CF0134A CRC64;
     MSEKPLTKTD YLMRLRRCQT IDTLERVIEK NKYELSDNEL AVFYSAADHR LAELTMNKLY
     DKIPSSVWKF IR