HHEX_DANRE
ID HHEX_DANRE Reviewed; 228 AA.
AC Q9IAV3;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Hematopoietically-expressed homeobox protein hhex;
DE Short=Homeobox protein hex;
GN Name=hhex {ECO:0000312|EMBL:AAF28383.1};
GN Synonyms=hex {ECO:0000303|PubMed:10531010};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAF28383.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Pharyngula {ECO:0000269|PubMed:10531010};
RX PubMed=10531010; DOI=10.1016/s0960-9822(99)80485-0;
RA Ho C.-Y., Houart C., Wilson S.W., Stainier D.Y.R.;
RT "A role for the extraembryonic yolk syncytial layer in patterning the
RT zebrafish embryo suggested by properties of the hex gene.";
RL Curr. Biol. 9:1131-1134(1999).
RN [2] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11003831; DOI=10.1242/dev.127.20.4303;
RA Liao W., Ho C.-Y., Yan Y.L., Postlethwait J.H., Stainier D.Y.R.;
RT "Hhex and scl function in parallel to regulate early endothelial and blood
RT differentiation in zebrafish.";
RL Development 127:4303-4313(2000).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=11477693; DOI=10.1002/gene.1050;
RA Wallace K.N., Yusuff S., Sonntag J.M., Chin A.J., Pack M.;
RT "Zebrafish hhex regulates liver development and digestive organ
RT chirality.";
RL Genesis 30:141-143(2001).
RN [4] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14568547; DOI=10.1016/s0012-1606(03)00436-6;
RA Elsalini O.A., von Gartzen J., Cramer M., Rohr K.B.;
RT "Zebrafish hhex, nk2.1a, and pax2.1 regulate thyroid growth and
RT differentiation downstream of Nodal-dependent transcription factors.";
RL Dev. Biol. 263:67-80(2003).
RN [5] {ECO:0000305}
RP INDUCTION.
RX PubMed=15581885; DOI=10.1016/j.ydbio.2004.09.035;
RA Bischof J., Driever W.;
RT "Regulation of hhex expression in the yolk syncytial layer, the potential
RT Nieuwkoop center homolog in zebrafish.";
RL Dev. Biol. 276:552-562(2004).
CC -!- FUNCTION: Recognizes the DNA sequence 5'-ATTAA-3' (By similarity).
CC Transcriptional repressor. Regulates the differentiation of both
CC endothelial and blood cells. Plays a role in embryonic dorsoventral
CC patterning by regulating bmp expression. May establish anterior
CC identity. Functions in the embryo to regulate liver development.
CC Functions extraembryonically to generate organ chirality.
CC {ECO:0000250|UniProtKB:Q05502, ECO:0000269|PubMed:10531010,
CC ECO:0000269|PubMed:11003831, ECO:0000269|PubMed:11477693,
CC ECO:0000269|PubMed:14568547}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in embryonic endothelial and blood
CC lineages. From late-blastula stage, expression is restricted to the
CC dorsal marginal region of the extraembryonic yolk syncytial layer
CC (YSL). By the onset of gastrulation, expressed in the entire dorsal
CC half of the YSL. Post-gastrulation, expression appears in both anterior
CC and posterior lateral plate mesoderm by the 3-somite stage.
CC Posteriorly, expression is in the intermediate cell mass (ICM), which
CC contains both endothelial and blood precursors. Subsequently expressed
CC in the developing endothelial cells including the endocardium until the
CC onset of circulation (24 hpf) and disappears completely by 30 hpf, at
CC which point expression is seen in the thyroid and liver primordia. Also
CC expressed in the developing biliary tree and pancreas.
CC {ECO:0000269|PubMed:10531010, ECO:0000269|PubMed:11003831,
CC ECO:0000269|PubMed:14568547}.
CC -!- INDUCTION: By dhama/boz downstream of canonical wnt-signaling.
CC {ECO:0000269|PubMed:15581885}.
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DR EMBL; AF131070; AAF28383.1; -; mRNA.
DR RefSeq; NP_571009.1; NM_130934.1.
DR AlphaFoldDB; Q9IAV3; -.
DR SMR; Q9IAV3; -.
DR GeneID; 30098; -.
DR KEGG; dre:30098; -.
DR CTD; 3087; -.
DR ZFIN; ZDB-GENE-980526-299; hhex.
DR InParanoid; Q9IAV3; -.
DR OrthoDB; 1596695at2759; -.
DR PhylomeDB; Q9IAV3; -.
DR PRO; PR:Q9IAV3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:UniProtKB.
DR GO; GO:0048565; P:digestive tract development; IMP:UniProtKB.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:UniProtKB.
DR GO; GO:0035162; P:embryonic hemopoiesis; IMP:UniProtKB.
DR GO; GO:0031017; P:exocrine pancreas development; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0001889; P:liver development; IMP:UniProtKB.
DR GO; GO:0001946; P:lymphangiogenesis; IMP:ZFIN.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0045603; P:positive regulation of endothelial cell differentiation; IMP:ZFIN.
DR GO; GO:0046620; P:regulation of organ growth; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0002040; P:sprouting angiogenesis; IMP:ZFIN.
DR GO; GO:0030878; P:thyroid gland development; IMP:ZFIN.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..228
FT /note="Hematopoietically-expressed homeobox protein hhex"
FT /id="PRO_0000326219"
FT DNA_BIND 117..176
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 175..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 228 AA; 26048 MW; 9F44C99F9772898B CRC64;
MQFQHPHAPL YAPAPAPPAH PTPFYIEDIL GRTGSSSGPV VPTPTLPSPN SSFTSLIPSY
RTPIYELTPI HPVLSQYASM YPFQRSVGDF AHALIRHDPL GKPLLWSPFI QRPLHKRKGG
QVRFSNDQTI ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
PSTGKREAED SDTRRLSDAA ARARELESGA STDSEELLDI EDEHQFTL
