HHY1_YEAST
ID HHY1_YEAST Reviewed; 102 AA.
AC P39982; A0A8D9PCP9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Hypersensitivity to hygromycin-B protein 1 {ECO:0000303|PubMed:20043226};
DE Flags: Precursor;
GN Name=HHY1 {ECO:0000303|PubMed:20043226};
GN OrderedLocusNames=YEL059W {ECO:0000312|SGD:S000000785};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [4]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=20043226; DOI=10.1007/s00294-009-0285-3;
RA Banuelos M.G., Moreno D.E., Olson D.K., Nguyen Q., Ricarte F.,
RA Aguilera-Sandoval C.R., Gharakhanian E.;
RT "Genomic analysis of severe hypersensitivity to hygromycin B reveals
RT linkage to vacuolar defects and new vacuolar gene functions in
RT Saccharomyces cerevisiae.";
RL Curr. Genet. 56:121-137(2010).
CC -!- FUNCTION: Involved in vacuolar trafficking.
CC {ECO:0000269|PubMed:20043226}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Leads to hygromycin B hypersentsitivity,
CC increased vacuolar fragmentation, defective CPY processing, and extreme
CC sensitivities to pH extremes, divalent cations, and multiple drugs.
CC {ECO:0000269|PubMed:20043226}.
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DR EMBL; U18795; AAB65028.1; -; Genomic_DNA.
DR EMBL; BK006939; DAD54804.1; -; Genomic_DNA.
DR PIR; S50530; S50530.
DR AlphaFoldDB; P39982; -.
DR DIP; DIP-5050N; -.
DR IntAct; P39982; 2.
DR STRING; 4932.YEL059W; -.
DR PaxDb; P39982; -.
DR EnsemblFungi; YEL059W_mRNA; YEL059W; YEL059W.
DR SGD; S000000785; HHY1.
DR HOGENOM; CLU_2279674_0_0_1; -.
DR PRO; PR:P39982; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P39982; protein.
DR GO; GO:0016021; C:integral component of membrane; ISM:SGD.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..102
FT /note="Hypersensitivity to hygromycin-B protein 1"
FT /id="PRO_0000202603"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:16847258"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..102
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:16847258"
SQ SEQUENCE 102 AA; 11341 MW; C5EF6ECA78E2DB9A CRC64;
MSLSFLLFSP FLPPCFSSIS ICLSVLSTVS FFFAFTIPHY VLRCGSVDEW HIHSSAEDFR
TQRCVCAVKL SASLLGCLLA CASWSLLLEV SRIKWHVGTA YS
