HH_DROGR
ID HH_DROGR Reviewed; 479 AA.
AC B4JTF5;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Protein hedgehog {ECO:0000250|UniProtKB:Q02936};
DE EC=3.1.-.- {ECO:0000250|UniProtKB:Q62226};
DE Contains:
DE RecName: Full=Protein hedgehog N-product {ECO:0000250|UniProtKB:Q02936};
DE Flags: Precursor;
GN Name=hh {ECO:0000250|UniProtKB:Q02936}; ORFNames=GH23852;
OS Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Hawaiian Drosophila.
OX NCBI_TaxID=7222;
RN [1] {ECO:0000312|EMBL:EDV95045.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15287-2541.00 {ECO:0000312|EMBL:EDV95045.1};
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: [Protein hedgehog]: The C-terminal part of the hedgehog
CC protein precursor displays an autoproteolysis activity that results in
CC the cleavage of the full-length protein into two parts (N-product and
CC C-product) (By similarity). In addition, the C-terminal part displays a
CC cholesterol transferase activity that results by the covalent
CC attachment of a cholesterol moiety to the C-terminal of the newly
CC generated N-product (By similarity). Once cleaved, the C-product has no
CC signaling activity and diffuses from the cell (By similarity).
CC {ECO:0000250|UniProtKB:Q02936, ECO:0000250|UniProtKB:Q62226}.
CC -!- FUNCTION: [Protein hedgehog N-product]: The dually lipidated hedgehog
CC protein N-product is a morphogen which is essential for a variety of
CC patterning events during development. Establishes the anterior-
CC posterior axis of the embryonic segments and patterns the larval
CC imaginal disks. Binds to the patched (ptc) receptor, which functions in
CC association with smoothened (smo), to activate the transcription of
CC target genes wingless (wg), decapentaplegic (dpp) and ptc. In the
CC absence of hh, ptc represses the constitutive signaling activity of smo
CC through fused (fu). Essential component of a signaling pathway which
CC regulates the Duox-dependent gut immune response to bacterial uracil;
CC required to activate Cad99C-dependent endosome formation, norpA-
CC dependent Ca2+ mobilization and p38 MAPK, which are essential steps in
CC the Duox-dependent production of reactive oxygen species (ROS) in
CC response to intestinal bacterial infection. During photoreceptor
CC differentiation, it up-regulates transcription of Ubr3, which in turn
CC promotes the hh-signaling pathway by mediating the ubiquitination and
CC degradation of cos. {ECO:0000250|UniProtKB:Q02936}.
CC -!- CATALYTIC ACTIVITY: [Protein hedgehog]:
CC Reaction=cholesterol + glycyl-L-cysteinyl-[protein] + H(+) = [protein]-
CC C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-
CC [protein]; Xref=Rhea:RHEA:59504, Rhea:RHEA-COMP:12707, Rhea:RHEA-
CC COMP:15369, Rhea:RHEA-COMP:15374, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16113, ChEBI:CHEBI:65250, ChEBI:CHEBI:143135,
CC ChEBI:CHEBI:143140; Evidence={ECO:0000250|UniProtKB:Q62226};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59505;
CC Evidence={ECO:0000250|UniProtKB:Q62226};
CC -!- SUBUNIT: Interacts with shf. {ECO:0000250|UniProtKB:Q02936}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q02936}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q02936}. Note=Nuclear up to embryonic stage 10
CC and then at stage 11 shifts to the cytoplasm. Also secreted in either
CC cleaved or uncleaved form to mediate signaling to other cells.
CC {ECO:0000250|UniProtKB:Q02936}.
CC -!- SUBCELLULAR LOCATION: [Protein hedgehog N-product]: Cell membrane
CC {ECO:0000250|UniProtKB:Q02936}; Lipid-anchor
CC {ECO:0000250|UniProtKB:Q02936}. Note=The N-terminal peptide remains
CC associated with the cell surface. Heparan sulfate proteoglycans of the
CC extracellular matrix play an essential role in diffusion. Lipophorin is
CC required for diffusion, probably by acting as vehicle for its movement,
CC explaining how it can spread over long distances despite its
CC lipidation. {ECO:0000250|UniProtKB:Q02936}.
CC -!- PTM: [Protein hedgehog]: The C-terminal part of the hedgehog protein
CC precursor displays an autoproteolysis activity that results in the
CC cleavage of the full-length protein into two parts (N-product and C-
CC product) (By similarity). In addition, the C-terminal part displays a
CC cholesterol transferase activity that results by the covalent
CC attachment of a cholesterol moiety to the C-terminal of the newly
CC generated N-product (By similarity). The N-product is the active
CC species in both local and long-range signaling, whereas the C-product
CC has no signaling activity (By similarity).
CC {ECO:0000250|UniProtKB:Q02936, ECO:0000250|UniProtKB:Q15465,
CC ECO:0000250|UniProtKB:Q62226}.
CC -!- PTM: [Protein hedgehog N-product]: Cholesterylation is required for N-
CC product targeting to lipid rafts and multimerization.
CC {ECO:0000250|UniProtKB:Q62226}.
CC -!- PTM: [Protein hedgehog N-product]: N-palmitoylation by Rasp of the
CC hedgehog N-product, within the secretory pathway, is required for the
CC embryonic and larval patterning activities of the hedgehog signal.
CC {ECO:0000250|UniProtKB:Q02936}.
CC -!- SIMILARITY: Belongs to the hedgehog family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CH916373; EDV95045.1; -; Genomic_DNA.
DR RefSeq; XP_001994309.1; XM_001994273.1.
DR AlphaFoldDB; B4JTF5; -.
DR SMR; B4JTF5; -.
DR STRING; 7222.FBpp0157758; -.
DR MEROPS; C46.001; -.
DR EnsemblMetazoa; FBtr0159266; FBpp0157758; FBgn0131309.
DR GeneID; 6567467; -.
DR KEGG; dgr:6567467; -.
DR eggNOG; KOG3638; Eukaryota.
DR HOGENOM; CLU_034686_0_0_1; -.
DR InParanoid; B4JTF5; -.
DR OMA; APAVRGC; -.
DR OrthoDB; 1169356at2759; -.
DR PhylomeDB; B4JTF5; -.
DR Proteomes; UP000001070; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016015; F:morphogen activity; IEA:UniProtKB-KW.
DR GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IEA:UniProtKB-KW.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0016540; P:protein autoprocessing; IEA:InterPro.
DR GO; GO:0007367; P:segment polarity determination; ISS:UniProtKB.
DR GO; GO:0048731; P:system development; IEA:UniProt.
DR Gene3D; 3.30.1380.10; -; 1.
DR InterPro; IPR001657; Hedgehog.
DR InterPro; IPR001767; Hedgehog_Hint.
DR InterPro; IPR009045; Hedgehog_sig/DD-Pept_Zn-bd_sf.
DR InterPro; IPR000320; Hedgehog_signalling_dom.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR006141; Intein_N.
DR Pfam; PF01085; HH_signal; 1.
DR Pfam; PF01079; Hint; 1.
DR PIRSF; PIRSF009400; Peptidase_C46; 1.
DR PRINTS; PR00632; SONICHHOG.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF55166; SSF55166; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Calcium; Cell membrane; Cytoplasm;
KW Developmental protein; Hydrolase; Lipoprotein; Membrane; Metal-binding;
KW Morphogen; Nucleus; Palmitate; Protease; Reference proteome;
KW Segmentation polarity protein; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..83
FT /evidence="ECO:0000255"
FT /id="PRO_0000383058"
FT CHAIN 84..479
FT /note="Protein hedgehog"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT /id="PRO_0000383059"
FT CHAIN 84..257
FT /note="Protein hedgehog N-product"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT /id="PRO_0000383060"
FT BINDING 148
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 149
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 149
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 154
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 184
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 185
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 185
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT BINDING 190
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q15465"
FT SITE 257..258
FT /note="Cleavage; by autolysis"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT SITE 303
FT /note="Involved in cholesterol transfer"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT SITE 326
FT /note="Involved in auto-cleavage"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT SITE 329
FT /note="Essential for auto-cleavage"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT LIPID 84
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
FT LIPID 257
FT /note="Cholesterol glycine ester"
FT /evidence="ECO:0000250|UniProtKB:Q02936"
SQ SEQUENCE 479 AA; 53441 MW; 9F21AF0506503C16 CRC64;
MDNEAVSALW SCASATCLSL DVKRHCSDAN VDSQARTQSQ ATPMTNETDP RKLRHIAHTP
RGSCFMTLLL LLLLALNFRH AHSCGPGRGL GRRRDRNLYP LVLKQTVPNL SEYQSGASGP
LEGVIDRKSP KFKDLVPLYN SDILFRDEEG TGADRMMTKR CKEKLVMLAT SVMNEWPGVK
LLVTESWDED HHHGEQSLHY EGRAVTIATS DRDQSKYGML ARLAVEAGFD WVSYVSRRHI
YCSVKSDSSS SISHVHGCFT PESTAQLESG AKKPLGELAI GDRVLSMDAK GQAVYSEVIL
FMDRNLEQME TFVQLHTDGG AVLTVTPAHL ITVWQPERQT LDFVFADHVE ELNYVLVVDD
ATGGELRPQR VLRVSSVRRR GVVAPLTREG TIVVNSVAAS CYAVISSQSL AHWGLAPMRL
WSTLQSWLPA KDKLRSSKVQ EKSTPKVNST AQLQNGLHWY ANALYKVKDY VLPQSWRHD
