HICB_ECOLI
ID HICB_ECOLI Reviewed; 138 AA.
AC P67697; P76107; Q2MBB7;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Antitoxin HicB;
GN Name=hicB; Synonyms=ydcQ; OrderedLocusNames=b1438, JW1433;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP PREDICTION OF FUNCTION.
RX PubMed=16895922; DOI=10.1093/bioinformatics/btl418;
RA Makarova K.S., Grishin N.V., Koonin E.V.;
RT "The HicAB cassette, a putative novel, RNA-targeting toxin-antitoxin system
RT in archaea and bacteria.";
RL Bioinformatics 22:2581-2584(2006).
RN [4]
RP SUPPRESSION OF RPOE ESSENTIALITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=17172327; DOI=10.1128/jb.01534-06;
RA Button J.E., Silhavy T.J., Ruiz N.;
RT "A suppressor of cell death caused by the loss of sigmaE downregulates
RT extracytoplasmic stress responses and outer membrane vesicle production in
RT Escherichia coli.";
RL J. Bacteriol. 189:1523-1530(2007).
RN [5]
RP FUNCTION AS AN MRNA INTERFERASE ANTITOXIN, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19060138; DOI=10.1128/jb.01013-08;
RA Jorgensen M.G., Pandey D.P., Jaskolska M., Gerdes K.;
RT "HicA of Escherichia coli defines a novel family of translation-independent
RT mRNA interferases in bacteria and archaea.";
RL J. Bacteriol. 191:1191-1199(2009).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC Functions as an mRNA interferase antitoxin; overexpression prevents
CC HicA-mediated cessation of cell growth and inhibition of cell
CC proliferation. {ECO:0000269|PubMed:19060138}.
CC -!- SUBUNIT: Probably forms a complex with the mRNA interferase HicA; when
CC the 2 dissociate the mRNA interferase becomes active. {ECO:0000250}.
CC -!- INDUCTION: Induced by amino acid starvation, carbon starvation and when
CC translation is blocked. Induction no longer occurs in the absence of
CC Lon protease suggesting, by homology to other toxin-antitoxin systems,
CC that Lon may degrade the HicB antitoxin. A member of the hicA-hicB
CC operon. {ECO:0000269|PubMed:19060138}.
CC -!- PTM: May be degraded by Lon protease. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Disruption suppresses an rpoE disruption; in a
CC wild-type background disruption down-regulates extracytoplasmic stress
CC responses and outer membrane vesicle production.
CC {ECO:0000269|PubMed:17172327}.
CC -!- SIMILARITY: Belongs to the HicB antitoxin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE76439.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00096; AAC74520.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76439.1; ALT_INIT; Genomic_DNA.
DR PIR; A64896; A64896.
DR RefSeq; NP_415955.2; NC_000913.3.
DR RefSeq; WP_001270286.1; NZ_SSZK01000021.1.
DR PDB; 6HPB; X-ray; 2.28 A; B/D=1-138.
DR PDB; 6HPC; X-ray; 2.26 A; A/B=1-138.
DR PDBsum; 6HPB; -.
DR PDBsum; 6HPC; -.
DR AlphaFoldDB; P67697; -.
DR SMR; P67697; -.
DR BioGRID; 4259496; 3.
DR BioGRID; 850362; 1.
DR ComplexPortal; CPX-4119; HicAB toxin-antitoxin complex.
DR DIP; DIP-48269N; -.
DR IntAct; P67697; 1.
DR STRING; 511145.b1438; -.
DR PaxDb; P67697; -.
DR PRIDE; P67697; -.
DR EnsemblBacteria; AAC74520; AAC74520; b1438.
DR EnsemblBacteria; BAE76439; BAE76439; BAE76439.
DR GeneID; 66674711; -.
DR GeneID; 946001; -.
DR KEGG; ecj:JW1433; -.
DR KEGG; eco:b1438; -.
DR PATRIC; fig|511145.12.peg.1503; -.
DR EchoBASE; EB3523; -.
DR eggNOG; COG1598; Bacteria.
DR HOGENOM; CLU_140890_2_0_6; -.
DR OMA; AFEFYFE; -.
DR BioCyc; EcoCyc:G6749-MON; -.
DR PRO; PR:P67697; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; IC:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:ComplexPortal.
DR CDD; cd00093; HTH_XRE; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR031807; HicB-like.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR035069; TTHA1013/TTHA0281-like.
DR Pfam; PF15919; HicB_lk_antitox; 1.
DR Pfam; PF01381; HTH_3; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF143100; SSF143100; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Stress response;
KW Toxin-antitoxin system; Transcription; Transcription regulation.
FT CHAIN 1..138
FT /note="Antitoxin HicB"
FT /id="PRO_0000149757"
FT DOMAIN 82..136
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 93..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT STRAND 2..9
FT /evidence="ECO:0007829|PDB:6HPC"
FT STRAND 13..21
FT /evidence="ECO:0007829|PDB:6HPC"
FT STRAND 26..31
FT /evidence="ECO:0007829|PDB:6HPC"
FT HELIX 32..53
FT /evidence="ECO:0007829|PDB:6HPC"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:6HPC"
FT HELIX 74..89
FT /evidence="ECO:0007829|PDB:6HPC"
FT HELIX 93..100
FT /evidence="ECO:0007829|PDB:6HPC"
FT HELIX 105..110
FT /evidence="ECO:0007829|PDB:6HPC"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:6HPB"
FT HELIX 119..128
FT /evidence="ECO:0007829|PDB:6HPC"
FT STRAND 132..137
FT /evidence="ECO:0007829|PDB:6HPC"
SQ SEQUENCE 138 AA; 15247 MW; C7B6C1F0D0A8BE26 CRC64;
MRYPVTLTPA PEGGYMVSFV DIPEALTQGE TVAEAMEAAK DALLTAFDFY FEDNELIPLP
SPLNSHDHFI EVPLSVASKV LLLNAFLQSE ITQQELARRI GKPKQEITRL FNLHHATKID
AVQLAAKALG KELSLVMV
