ID   HIGA1_VIBCH             Reviewed;         105 AA.
AC   Q9KMG4;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Antitoxin HigA-1;
GN   Name=higA-1; OrderedLocusNames=VC_A0392;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor Inaba V812 / Serotype O1;
RA   Labbate M., Boucher Y., Joss M.J., Michael C., Gillings M.R., Stokes H.W.;
RT   "Evolution of chromosomal integron cassette arrays in Vibrio cholerae
RT   pandemic strains.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=17020579; DOI=10.1111/j.1365-2958.2006.05385.x;
RA   Christensen-Dalsgaard M., Gerdes K.;
RT   "Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving
RT   enzymes and can stabilize plasmids.";
RL   Mol. Microbiol. 62:397-411(2006).
RN   [4]
RP   FUNCTION, INDUCTION, AND INTERACTION WITH HIGB-1.
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=17085558; DOI=10.1128/jb.00909-06;
RA   Budde P.P., Davis B.M., Yuan J., Waldor M.K.;
RT   "Characterization of a higBA toxin-antitoxin locus in Vibrio cholerae.";
RL   J. Bacteriol. 189:491-500(2007).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system
CC       that counteracts the effect of the HigB-1 toxin. Binds to its own
CC       promoter and regulates transcription of the higB-1/higA-1 operon.
CC       {ECO:0000269|PubMed:17020579, ECO:0000269|PubMed:17085558}.
CC   -!- INDUCTION: Induced by amino acid starvation. Induced by the protein
CC       synthesis inhibitors chloramphenicol, kanamycin and to a lesser extent
CC       by spectinomycin. {ECO:0000269|PubMed:17020579,
CC       ECO:0000269|PubMed:17085558}.
CC   -!- MISCELLANEOUS: HigB-1/HigA-1 has been shown to stabilize plasmids very
CC       efficiently in E.coli.
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DR   EMBL; DQ513173; ABF72536.1; -; Genomic_DNA.
DR   EMBL; AE003853; AAF96298.1; -; Genomic_DNA.
DR   PIR; E82467; E82467.
DR   RefSeq; NP_232786.1; NC_002506.1.
DR   RefSeq; WP_001232701.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KMG4; -.
DR   SMR; Q9KMG4; -.
DR   STRING; 243277.VC_A0392; -.
DR   DNASU; 2612448; -.
DR   EnsemblBacteria; AAF96298; AAF96298; VC_A0392.
DR   GeneID; 57741821; -.
DR   KEGG; vch:VC_A0392; -.
DR   PATRIC; fig|243277.26.peg.3022; -.
DR   eggNOG; COG3093; Bacteria.
DR   HOGENOM; CLU_140230_3_2_6; -.
DR   OMA; GNSAKFW; -.
DR   BioCyc; VCHO:VCA0392-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013430; Toxin_antidote_HigA.
DR   PANTHER; PTHR36924; PTHR36924; 1.
DR   Pfam; PF01381; HTH_3; 1.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   TIGRFAMs; TIGR02607; antidote_HigA; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..105
FT                   /note="Antitoxin HigA-1"
FT                   /id="PRO_0000278766"
FT   DOMAIN          15..69
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        27..46
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
SQ   SEQUENCE   105 AA;  11706 MW;  4160F7DB82F8AA86 CRC64;
     MRKTKRRPVS VGEMLKVEFL EPMGITSKAL AEAMGVHRNT VSNLINGGVL TAPVAIKLAA
     ALGNTPEFWL NIQHAVDLWD TRNRYQEEAK FVKPLFVSLE QSART