ID   HIGA2_MYCTU             Reviewed;         101 AA.
AC   O53467; F2GG69; I6X2Z5; Q7D7M1;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Putative antitoxin HigA2 {ECO:0000303|PubMed:24662523};
GN   Name=higA2 {ECO:0000303|PubMed:24662523};
GN   OrderedLocusNames=Rv2021c, RVBD_2021c; ORFNames=LH57_11010, P425_02092;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genome Sequencing Platform;
RA   Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA   Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genomics Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA   Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA   Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA   Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INDUCTION IN PERSISTER CELLS.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21673191; DOI=10.1128/mbio.00100-11;
RA   Keren I., Minami S., Rubin E., Lewis K.;
RT   "Characterization and transcriptome analysis of Mycobacterium tuberculosis
RT   persisters.";
RL   MBio 2:E00100-E00111(2011).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [7]
RP   DISCUSSION OF POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=24662523; DOI=10.3390/toxins6031002;
RA   Sala A., Bordes P., Genevaux P.;
RT   "Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.";
RL   Toxins 6:1002-1020(2014).
CC   -!- FUNCTION: Putative antitoxin component of a type II toxin-antitoxin
CC       (TA) system. Its cognate toxin would be HigB2.
CC       {ECO:0000305|PubMed:24662523}.
CC   -!- INDUCTION: Induced in persister cells in response to D-cycloserine.
CC       {ECO:0000269|PubMed:21673191}.
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DR   EMBL; AL123456; CCP44793.1; -; Genomic_DNA.
DR   EMBL; CP003248; AFN49961.1; -; Genomic_DNA.
DR   EMBL; CP009480; AIR14771.1; -; Genomic_DNA.
DR   EMBL; JLDD01000023; KBJ33165.1; -; Genomic_DNA.
DR   RefSeq; NP_216537.1; NC_000962.3.
DR   RefSeq; WP_003410124.1; NZ_NVQJ01000046.1.
DR   PDB; 7EWC; X-ray; 2.05 A; A/B/C/D=1-101.
DR   PDB; 7EWD; X-ray; 3.20 A; A/B/C/D=1-101.
DR   PDB; 7EWE; X-ray; 3.41 A; A/B/C/D/E=1-101.
DR   PDBsum; 7EWC; -.
DR   PDBsum; 7EWD; -.
DR   PDBsum; 7EWE; -.
DR   AlphaFoldDB; O53467; -.
DR   SMR; O53467; -.
DR   STRING; 83332.Rv2021c; -.
DR   PaxDb; O53467; -.
DR   DNASU; 888092; -.
DR   GeneID; 888092; -.
DR   KEGG; mtu:Rv2021c; -.
DR   KEGG; mtv:RVBD_2021c; -.
DR   PATRIC; fig|83332.111.peg.2252; -.
DR   TubercuList; Rv2021c; -.
DR   eggNOG; COG1396; Bacteria.
DR   HOGENOM; CLU_066192_13_0_11; -.
DR   OMA; NRVSQME; -.
DR   PhylomeDB; O53467; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:MTBBASE.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR039554; HigA2-like_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   Pfam; PF13744; HTH_37; 1.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..101
FT                   /note="Putative antitoxin HigA2"
FT                   /id="PRO_0000432911"
FT   DOMAIN          35..90
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        46..65
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
SQ   SEQUENCE   101 AA;  11292 MW;  B98C059D8DC75E83 CRC64;
     MAMTLRDMDA VRPVNREAVD RHKARMRDEV RAFRLRELRA AQSLTQVQVA ALAHIRQSRV
     SSIENGDIGS AQVNTLRKYV SALGGELDIT VRLGDETFTL A