ID   HIGA2_VIBCH             Reviewed;         104 AA.
AC   Q9KMA5;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Antitoxin HigA-2;
GN   Name=higA-2; OrderedLocusNames=VC_A0469;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=17020579; DOI=10.1111/j.1365-2958.2006.05385.x;
RA   Christensen-Dalsgaard M., Gerdes K.;
RT   "Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving
RT   enzymes and can stabilize plasmids.";
RL   Mol. Microbiol. 62:397-411(2006).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system
CC       that counteracts the effect of the HigB-2 toxin. Binds to its own
CC       promoter and regulates transcription of the higB-2/higA-2 operon.
CC       {ECO:0000269|PubMed:17020579}.
CC   -!- INDUCTION: Induced by amino acid starvation and the protein synthesis
CC       inhibitor chloramphenicol. {ECO:0000269|PubMed:17020579}.
CC   -!- MISCELLANEOUS: HigB-2/HigA-2 has been shown to stabilize plasmids very
CC       efficiently in E.coli.
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DR   EMBL; AE003853; AAF96373.1; -; Genomic_DNA.
DR   PIR; E82455; E82455.
DR   RefSeq; NP_232861.1; NC_002506.1.
DR   RefSeq; WP_000071008.1; NZ_LT906615.1.
DR   PDB; 5J9I; X-ray; 1.80 A; A/B/C/D/E/F/G/H=1-104.
DR   PDB; 5JAA; X-ray; 2.99 A; A/B=2-104.
DR   PDBsum; 5J9I; -.
DR   PDBsum; 5JAA; -.
DR   AlphaFoldDB; Q9KMA5; -.
DR   SMR; Q9KMA5; -.
DR   STRING; 243277.VC_A0469; -.
DR   PRIDE; Q9KMA5; -.
DR   DNASU; 2611844; -.
DR   EnsemblBacteria; AAF96373; AAF96373; VC_A0469.
DR   GeneID; 57741883; -.
DR   GeneID; 57992134; -.
DR   KEGG; vch:VC_A0469; -.
DR   PATRIC; fig|243277.26.peg.3095; -.
DR   eggNOG; COG2944; Bacteria.
DR   HOGENOM; CLU_144725_3_1_6; -.
DR   OMA; WEQGRAK; -.
DR   BioCyc; VCHO:VCA0469-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00093; HTH_XRE; 1.
DR   DisProt; DP01289; -.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR032758; MqsA/HigA-2.
DR   Pfam; PF15731; MqsA_antitoxin; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Toxin-antitoxin system;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..104
FT                   /note="Antitoxin HigA-2"
FT                   /id="PRO_0000278767"
FT   DOMAIN          45..98
FT                   /note="HTH cro/C1-type"
FT   DNA_BIND        56..75
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   HELIX           6..21
FT                   /evidence="ECO:0007829|PDB:5JAA"
FT   STRAND          29..32
FT                   /evidence="ECO:0007829|PDB:5JAA"
FT   HELIX           42..51
FT                   /evidence="ECO:0007829|PDB:5J9I"
FT   HELIX           56..62
FT                   /evidence="ECO:0007829|PDB:5J9I"
FT   HELIX           67..74
FT                   /evidence="ECO:0007829|PDB:5J9I"
FT   HELIX           82..93
FT                   /evidence="ECO:0007829|PDB:5J9I"
FT   HELIX           96..102
FT                   /evidence="ECO:0007829|PDB:5J9I"
SQ   SEQUENCE   104 AA;  11696 MW;  9E6CC9D8FBEDDFAA CRC64;
     MSNRDLFAEL SSALVEAKQH SEGKLTLKTH HVNDVGELNI SPDEIVSIRE QFNMSRGVFA
     RLLHTSSRTL ENWEQGRSVP NGQAVTLLKL VQRHPETLSH IAEL