HIGA3_MYCTU
ID HIGA3_MYCTU Reviewed; 109 AA.
AC O53333; F2GJP2; I6X6L4; Q7D5Z3;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Putative antitoxin HigA3 {ECO:0000303|PubMed:24662523};
GN Name=higA3 {ECO:0000303|PubMed:24662523};
GN OrderedLocusNames=Rv3183, RVBD_3183; ORFNames=LH57_17370, P425_03315;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DISCUSSION OF POSSIBLE FUNCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=24662523; DOI=10.3390/toxins6031002;
RA Sala A., Bordes P., Genevaux P.;
RT "Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.";
RL Toxins 6:1002-1020(2014).
CC -!- FUNCTION: Putative antitoxin component of a type II toxin-antitoxin
CC (TA) system. Its cognate toxin would be HigB3.
CC {ECO:0000305|PubMed:24662523}.
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DR EMBL; AL123456; CCP45994.1; -; Genomic_DNA.
DR EMBL; CP003248; AFN51176.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR15968.1; -; Genomic_DNA.
DR EMBL; JLDD01000038; KBJ29229.1; -; Genomic_DNA.
DR RefSeq; NP_217699.1; NC_000962.3.
DR RefSeq; WP_003899955.1; NZ_NVQJ01000019.1.
DR PDB; 6LTY; X-ray; 3.28 A; A/B=1-109.
DR PDB; 6LTZ; X-ray; 1.97 A; A/B=1-109.
DR PDBsum; 6LTY; -.
DR PDBsum; 6LTZ; -.
DR AlphaFoldDB; O53333; -.
DR SMR; O53333; -.
DR STRING; 83332.Rv3183; -.
DR PaxDb; O53333; -.
DR DNASU; 888059; -.
DR GeneID; 45427172; -.
DR GeneID; 888059; -.
DR KEGG; mtu:Rv3183; -.
DR KEGG; mtv:RVBD_3183; -.
DR PATRIC; fig|83332.111.peg.3546; -.
DR TubercuList; Rv3183; -.
DR eggNOG; COG1396; Bacteria.
DR HOGENOM; CLU_066192_13_0_11; -.
DR OMA; WASAYHL; -.
DR PhylomeDB; O53333; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR039554; HigA2-like_HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR Pfam; PF13744; HTH_37; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Toxin-antitoxin system;
KW Transcription; Transcription regulation.
FT CHAIN 1..109
FT /note="Putative antitoxin HigA3"
FT /id="PRO_0000432912"
FT DOMAIN 41..97
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 53..72
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT STRAND 37..40
FT /evidence="ECO:0007829|PDB:6LTZ"
FT HELIX 41..47
FT /evidence="ECO:0007829|PDB:6LTZ"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:6LTY"
FT HELIX 53..60
FT /evidence="ECO:0007829|PDB:6LTZ"
FT HELIX 64..72
FT /evidence="ECO:0007829|PDB:6LTZ"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:6LTZ"
FT HELIX 80..89
FT /evidence="ECO:0007829|PDB:6LTZ"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:6LTZ"
FT STRAND 103..107
FT /evidence="ECO:0007829|PDB:6LTZ"
SQ SEQUENCE 109 AA; 11802 MW; A66DBE160D79613A CRC64;
MTMARNWRDI RADAVAQGRV DLQRAAVARE EMRDAVLAHR LAEIRKALGH ARQADVAALM
GVSQARVSKL ESGDLSHTEL GTLQAYVAAL GGHLRIVAEF GENTVELTA
