HIGA_SHIFL
ID HIGA_SHIFL Reviewed; 138 AA.
AC P67703; P42594;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Antitoxin HigA;
GN Name=higA; OrderedLocusNames=SF3122, S3329;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC Functions as an mRNA interferase antitoxin.
CC {ECO:0000250|UniProtKB:P67701}.
CC -!- SUBUNIT: Probably forms a complex with the mRNA interferase HigB which
CC inhibits the mRNA interferase activity. {ECO:0000250|UniProtKB:P67701}.
CC -!- SIMILARITY: Belongs to the HigA antitoxin family. {ECO:0000305}.
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DR EMBL; AE005674; AAN44594.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP18407.1; -; Genomic_DNA.
DR RefSeq; NP_708887.1; NC_004337.2.
DR RefSeq; WP_000560266.1; NZ_WPGW01000031.1.
DR PDB; 5YCL; X-ray; 3.10 A; A/C=4-138.
DR PDB; 6IRP; X-ray; 1.95 A; A/B=1-138.
DR PDBsum; 5YCL; -.
DR PDBsum; 6IRP; -.
DR AlphaFoldDB; P67703; -.
DR SMR; P67703; -.
DR STRING; 198214.SF3122; -.
DR EnsemblBacteria; AAN44594; AAN44594; SF3122.
DR EnsemblBacteria; AAP18407; AAP18407; S3329.
DR GeneID; 1026701; -.
DR GeneID; 66673019; -.
DR KEGG; sfl:SF3122; -.
DR KEGG; sfx:S3329; -.
DR PATRIC; fig|198214.7.peg.3709; -.
DR HOGENOM; CLU_125852_0_0_6; -.
DR OMA; DIAQTWA; -.
DR OrthoDB; 1684303at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR039060; Antitox_HigA.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR40455; PTHR40455; 1.
DR Pfam; PF01381; HTH_3; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor;
KW Toxin-antitoxin system; Transcription; Transcription regulation.
FT CHAIN 1..138
FT /note="Antitoxin HigA"
FT /id="PRO_0000149765"
FT DOMAIN 84..136
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 95..114
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT HELIX 4..17
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 19..22
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 27..43
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 49..63
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 65..76
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 80..91
FT /evidence="ECO:0007829|PDB:6IRP"
FT TURN 99..101
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 104..111
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 119..129
FT /evidence="ECO:0007829|PDB:6IRP"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:6IRP"
SQ SEQUENCE 138 AA; 14995 MW; 4A14D9E43480DCB5 CRC64;
MIAIADILQA GEKLTAVAPF LAGIQNEEQY TQALELVDHL LLNDPENPLL DLVCAKITAW
EESAPEFAEF NAMAQAMPGG IAVIRTLMDQ YGLTLSDLPE IGSKSMVSRV LSGKRKLTLE
HAKKLATRFG ISPALFID
