ID   HIGB1_VIBCH             Reviewed;          95 AA.
AC   Q9KMG5;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Toxin HigB-1;
GN   Name=higB-1; OrderedLocusNames=VC_A0391;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=17020579; DOI=10.1111/j.1365-2958.2006.05385.x;
RA   Christensen-Dalsgaard M., Gerdes K.;
RT   "Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving
RT   enzymes and can stabilize plasmids.";
RL   Mol. Microbiol. 62:397-411(2006).
RN   [3]
RP   FUNCTION, INDUCTION, INTERACTION BETWEEN HIGA-1 AND HIGB-1, AND
RP   CHARACTERIZATION OF THE TRANSCRIPTIONAL START SITE.
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=17085558; DOI=10.1128/jb.00909-06;
RA   Budde P.P., Davis B.M., Yuan J., Waldor M.K.;
RT   "Characterization of a higBA toxin-antitoxin locus in Vibrio cholerae.";
RL   J. Bacteriol. 189:491-500(2007).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Inhibits translation by cleavage of mRNA. {ECO:0000269|PubMed:17020579,
CC       ECO:0000269|PubMed:17085558}.
CC   -!- INDUCTION: Induced by amino acid starvation. Induced by the protein
CC       synthesis inhibitors chloramphenicol, kanamycin and to a lesser extent
CC       by spectinomycin. {ECO:0000269|PubMed:17020579,
CC       ECO:0000269|PubMed:17085558}.
CC   -!- MISCELLANEOUS: HigB-1/HigA-1 has been shown to stabilize plasmids very
CC       efficiently in E.coli. Three hours of ectopic expression of higB-1
CC       results in bacteriostasis without any detectable loss of viability.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF96297.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE003853; AAF96297.1; ALT_INIT; Genomic_DNA.
DR   PIR; D82467; D82467.
DR   RefSeq; NP_232785.1; NC_002506.1.
DR   RefSeq; WP_001162670.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KMG5; -.
DR   SMR; Q9KMG5; -.
DR   STRING; 243277.VC_A0391; -.
DR   PRIDE; Q9KMG5; -.
DR   DNASU; 2612447; -.
DR   EnsemblBacteria; AAF96297; AAF96297; VC_A0391.
DR   GeneID; 57741820; -.
DR   KEGG; vch:VC_A0391; -.
DR   PATRIC; fig|243277.26.peg.3021; -.
DR   eggNOG; COG3549; Bacteria.
DR   HOGENOM; CLU_155111_1_0_6; -.
DR   OMA; WLRAFFV; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   Gene3D; 3.30.2310.20; -; 1.
DR   InterPro; IPR007711; HigB-1.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   PANTHER; PTHR40266; PTHR40266; 1.
DR   Pfam; PF05015; HigB-like_toxin; 1.
DR   SUPFAM; SSF143011; SSF143011; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..95
FT                   /note="Toxin HigB-1"
FT                   /id="PRO_0000278768"
SQ   SEQUENCE   95 AA;  11350 MW;  D820B19DAF3464A9 CRC64;
     MALEFKDKWL EQFYEDDKRH RLIPSSIENA LFRKLEILDA AQAESDLRIP PGNRFEHLEG
     NLKGWCSIRV NKQYRLIFQW VDGVALNTYL DPHKY