ID   HIGB_ECO57              Reviewed;         104 AA.
AC   P64579; P42595;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=mRNA interferase HigB;
DE            EC=3.1.-.-;
DE   AltName: Full=Endoribonuclease HigB;
DE   AltName: Full=Toxin HigB;
GN   Name=higB; OrderedLocusNames=Z4436, ECs3965;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. A
CC       probable translation-dependent mRNA interferase.
CC       {ECO:0000250|UniProtKB:P64578}.
CC   -!- SUBUNIT: Probably forms a complex with the antitoxin HigA which
CC       inhibits the mRNA interferase activity. {ECO:0000250|UniProtKB:P64578}.
CC   -!- SIMILARITY: Belongs to the HigB mRNA interferase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005174; AAG58216.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37388.1; -; Genomic_DNA.
DR   PIR; D85969; D85969.
DR   PIR; E91124; E91124.
DR   RefSeq; NP_311992.1; NC_002695.1.
DR   RefSeq; WP_000550189.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P64579; -.
DR   SMR; P64579; -.
DR   STRING; 155864.EDL933_4305; -.
DR   EnsemblBacteria; AAG58216; AAG58216; Z4436.
DR   EnsemblBacteria; BAB37388; BAB37388; ECs_3965.
DR   GeneID; 66506933; -.
DR   GeneID; 916202; -.
DR   KEGG; ece:Z4436; -.
DR   KEGG; ecs:ECs_3965; -.
DR   PATRIC; fig|386585.9.peg.4139; -.
DR   eggNOG; COG4680; Bacteria.
DR   HOGENOM; CLU_153067_0_1_6; -.
DR   OMA; VMFFADF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR018669; Toxin_HigB.
DR   Pfam; PF09907; HigB_toxin; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding;
KW   Toxin-antitoxin system.
FT   CHAIN           1..104
FT                   /note="mRNA interferase HigB"
FT                   /id="PRO_0000169422"
SQ   SEQUENCE   104 AA;  12103 MW;  F23FB5F433C52C22 CRC64;
     MHLITQKALK DAAEKYPQHK TELVALGNTI AKGYFKKPES LKAVFPSLDN FKYLDKHYVF
     NVGGNELRVV AMVFFESQKC YIREVMTHKE YDFFTAVHRT KGKK