ID   HIGB_ECOL6              Reviewed;         103 AA.
AC   Q8FEM8;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=mRNA interferase HigB;
DE            EC=3.1.-.-;
DE   AltName: Full=Endoribonuclease HigB;
DE   AltName: Full=Toxin HigB;
GN   Name=higB; OrderedLocusNames=c3274;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. A
CC       probable translation-dependent mRNA interferase. Probably counteracted
CC       by antitoxin HigA. {ECO:0000250|UniProtKB:P64578}.
CC   -!- SUBUNIT: Probably forms a complex with the antitoxin HigA which
CC       inhibits the mRNA interferase activity. {ECO:0000250|UniProtKB:P64578}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the higB-higA operon has no effect on
CC       virulence in mouse infection; the disrupted strain is as virulent as
CC       wild-type. {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the HigB mRNA interferase family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN81723.1; -; Genomic_DNA.
DR   RefSeq; WP_000547555.1; NC_004431.1.
DR   AlphaFoldDB; Q8FEM8; -.
DR   SMR; Q8FEM8; -.
DR   STRING; 199310.c3274; -.
DR   EnsemblBacteria; AAN81723; AAN81723; c3274.
DR   KEGG; ecc:c3274; -.
DR   eggNOG; COG4680; Bacteria.
DR   HOGENOM; CLU_153067_0_0_6; -.
DR   OMA; MHVISKE; -.
DR   BioCyc; ECOL199310:C3274-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR018669; Toxin_HigB.
DR   Pfam; PF09907; HigB_toxin; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..103
FT                   /note="mRNA interferase HigB"
FT                   /id="PRO_0000420795"
SQ   SEQUENCE   103 AA;  12439 MW;  DE0BE63AF02D2FC2 CRC64;
     MHIISKAPFE ECARKYPNDA LALHSLYRVI KETDFSTPEE MRTAFPNLDN FKYRNKWYVL
     DVGGNNLRVI AYINFVNKRF FVKHITNHAE YDKLTRYYRE NKE