ID   HIGB_SHIFL              Reviewed;         104 AA.
AC   P64580; P42595;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=mRNA interferase HigB;
DE            EC=3.1.-.-;
DE   AltName: Full=Endoribonuclease HigB;
DE   AltName: Full=Toxin HigB;
GN   Name=higB; OrderedLocusNames=SF3123, S3330;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. A
CC       probable translation-dependent mRNA interferase.
CC       {ECO:0000250|UniProtKB:P64578}.
CC   -!- SUBUNIT: Probably forms a complex with the antitoxin HigA which
CC       inhibits the mRNA interferase activity. {ECO:0000250|UniProtKB:P64578}.
CC   -!- SIMILARITY: Belongs to the HigB mRNA interferase family. {ECO:0000305}.
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DR   EMBL; AE005674; AAN44595.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18408.1; -; Genomic_DNA.
DR   RefSeq; NP_708888.2; NC_004337.2.
DR   RefSeq; WP_000550189.1; NZ_WPGW01000031.1.
DR   PDB; 5YCL; X-ray; 3.10 A; B/D=1-99.
DR   PDBsum; 5YCL; -.
DR   AlphaFoldDB; P64580; -.
DR   SMR; P64580; -.
DR   STRING; 198214.SF3123; -.
DR   EnsemblBacteria; AAN44595; AAN44595; SF3123.
DR   EnsemblBacteria; AAP18408; AAP18408; S3330.
DR   GeneID; 1026710; -.
DR   GeneID; 66506933; -.
DR   KEGG; sfl:SF3123; -.
DR   KEGG; sfx:S3330; -.
DR   PATRIC; fig|198214.7.peg.3710; -.
DR   HOGENOM; CLU_153067_0_1_6; -.
DR   OMA; VMFFADF; -.
DR   OrthoDB; 2072230at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR018669; Toxin_HigB.
DR   Pfam; PF09907; HigB_toxin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..104
FT                   /note="mRNA interferase HigB"
FT                   /id="PRO_0000169423"
FT   HELIX           7..15
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   HELIX           17..19
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   HELIX           20..30
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   HELIX           40..43
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   STRAND          57..62
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   TURN            63..66
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   STRAND          67..74
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   TURN            75..78
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   STRAND          79..87
FT                   /evidence="ECO:0007829|PDB:5YCL"
FT   HELIX           88..96
FT                   /evidence="ECO:0007829|PDB:5YCL"
SQ   SEQUENCE   104 AA;  12103 MW;  F23FB5F433C52C22 CRC64;
     MHLITQKALK DAAEKYPQHK TELVALGNTI AKGYFKKPES LKAVFPSLDN FKYLDKHYVF
     NVGGNELRVV AMVFFESQKC YIREVMTHKE YDFFTAVHRT KGKK